Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   V9L13_RS22825 Genome accession   NZ_CP146986
Coordinates   5041024..5043096 (+) Length   690 a.a.
NCBI ID   WP_338800601.1    Uniprot ID   -
Organism   Pseudomonas sp. RSB 5.4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5036024..5048096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9L13_RS22805 (V9L13_22805) pilM 5038236..5039300 (+) 1065 WP_338800599.1 pilus assembly protein PilM Machinery gene
  V9L13_RS22810 (V9L13_22810) - 5039300..5039866 (+) 567 WP_003220894.1 PilN domain-containing protein -
  V9L13_RS22815 (V9L13_22815) pilO 5039863..5040486 (+) 624 WP_003220896.1 type 4a pilus biogenesis protein PilO -
  V9L13_RS22820 (V9L13_22820) - 5040483..5041010 (+) 528 WP_338800600.1 pilus assembly protein PilP -
  V9L13_RS22825 (V9L13_22825) pilQ 5041024..5043096 (+) 2073 WP_338800601.1 type IV pilus secretin PilQ Machinery gene
  V9L13_RS22830 (V9L13_22830) aroK 5043101..5043619 (+) 519 WP_045122260.1 shikimate kinase AroK -
  V9L13_RS22835 (V9L13_22835) aroB 5043771..5044871 (+) 1101 WP_210556778.1 3-dehydroquinate synthase -
  V9L13_RS22840 (V9L13_22840) - 5044882..5046471 (+) 1590 WP_003220906.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74820.16 Da        Isoelectric Point: 5.5748

>NTDB_id=945389 V9L13_RS22825 WP_338800601.1 5041024..5043096(+) (pilQ) [Pseudomonas sp. RSB 5.4]
MNRIFSTLGFSLWIALLSPMVQAASLKALDVAALPGDRIELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLTSKTRD
LGSGNARTATVVEANDRTRLIINLTQLTPYTSRVEGNNLFVVVGQGAKPAAPRPAAAAPRPVAAPAKAYAPVAKAIRGVD
FQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPDRLRVRLDVKDFATPVQFVNAAATGDRAVITVEPSGTFD
YSTYQTDNKLTVSIRPMTVDDLQKRNAERQAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPW
DQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAKID
ERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSNGT
STTIGTPGSTSTNSPFVDMGTIGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
ASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=945389 V9L13_RS22825 WP_338800601.1 5041024..5043096(+) (pilQ) [Pseudomonas sp. RSB 5.4]
ATGAACAGGATTTTTTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGCTGTCACCGATGGTTCAGGCGGCCAGTCTCAA
GGCGCTGGATGTCGCGGCGTTACCGGGTGACCGCATCGAGTTGAAGCTGTCGTTCGACGGCCCACCACCGCAGCCCAAGG
GCTACACCACCGAATCTCCGGCGCGAATCGCGCTGGATCTGCCGGGTGTCGCCAGCCAGCTGACGAGCAAGACCCGCGAT
CTGGGCAGCGGCAATGCGCGAACGGCGACAGTGGTCGAGGCCAACGACCGCACGCGCCTGATCATCAACCTGACGCAACT
GACGCCCTACACCTCGCGGGTTGAGGGTAACAATCTGTTCGTGGTGGTCGGGCAGGGCGCCAAGCCTGCTGCGCCGCGCC
CAGCCGCTGCTGCGCCACGACCGGTCGCGGCTCCGGCGAAGGCTTACGCCCCCGTTGCTAAGGCCATTCGTGGCGTGGAC
TTCCAACGTGGCACCGCGGGCGAGGGCAACGTGGTCATTGATCTGTCGGACCCGACCATCGCCCCGGATATCCAGGAGCA
TGACGGCAAGATCATCCTCAGTTTTGCCCGCACCCAGTTGCCCGACAGGCTGCGGGTGCGCCTCGACGTCAAGGACTTCG
CCACGCCTGTGCAGTTCGTCAACGCCGCCGCCACCGGTGATCGTGCGGTCATCACCGTCGAACCCAGCGGCACGTTCGAC
TATTCCACCTACCAGACCGATAACAAACTGACCGTCAGCATTCGCCCGATGACGGTCGATGACCTGCAAAAACGCAATGC
CGAGCGCCAGGCCTACAGTGGCGAAAAACTCTCGCTGAATTTTCAGGACATCGATGTGCGCTCGGTGCTGCAACTGATCG
CCGATTTCACCAACCTCAACCTGGTGGCCAGCGATACGGTGCAAGGCGGTATTACCCTGCGTCTGCAAAACGTGCCATGG
GATCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGGCTGGATAAACGCAAGATCGGCAACGTGTTGCTGGTGGCGCCGGC
CGACGAAATCGCTGCCCGCGAGCGCCAGGAGCTGGAGTCGCAGAAACAGATCGCCGAACTGGCACCGCTGCGCCGCGAGC
TGCTGCAAGTGAACTACGCCAAGGCGGCGGACATCGCCAAGCTGTTCCAATCGGTGACCAGCGCCGAGGCGAAAATCGAC
GAGCGCGGCTCGATCACCGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGATGAACTGCG
GCGGATCGTGGCGCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCGCGGATTGTCGAGGCCAACGTCGATTACG
ACAAGAGTCTGGGTGTGCGCTGGGGCGGTTCGATCCAGAACAAGGGCAACTGGAACACCTCCGGGGTCAGCAATGGCACC
TCTACCACCATTGGCACACCGGGCAGCACCAGCACCAATTCGCCGTTCGTCGACATGGGCACGATCGGTAATACCTCGGG
GATCGGCATCGCCTTCATCACCGACAATGTGCTGCTCGATCTTGAGCTGACCGCCATGGAGAAGACCGGCAACGGTGAAA
TCGTCTCGCAGCCGAAAGTGGTTACCTCCGACAAAGAGACCGCAAAGATCCTCAAGGGCACCGAGATTCCGTATCAGGAA
GCCAGCTCCAGCGGCGCGACGTCGGTGTCGTTCAAGGAAGCTTCGCTGTCCCTGGAAGTAACGCCTCAGATCACCCCTGA
CAACCGCATCATCATGGAGGTCAAAGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTGCTGGTGAACGACGGCGAGACCATCGTGATTGGCGGTGTTTTCTCAAATACTCAA
AGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(374-439)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(300-346)

AMIN domain (AMIN)

(37-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.147

100

0.758
  comQ Acinetobacter baylyi ADP1

41.1

100

0.412
  pilQ Acinetobacter baumannii D1279779

37.852

100

0.409
  pilQ Acinetobacter baumannii strain A118

37.852

100

0.409
  pilQ Legionella pneumophila strain ERS1305867

38.152

97.246

0.371
  pilQ Legionella pneumophila strain Lp02

38.152

97.246

0.371