Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   V9L13_RS22805 Genome accession   NZ_CP146986
Coordinates   5038236..5039300 (+) Length   354 a.a.
NCBI ID   WP_338800599.1    Uniprot ID   -
Organism   Pseudomonas sp. RSB 5.4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5033236..5044300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9L13_RS22795 (V9L13_22795) - 5034040..5035308 (+) 1269 WP_338800598.1 malic enzyme-like NAD(P)-binding protein -
  V9L13_RS22800 (V9L13_22800) - 5035572..5038019 (-) 2448 WP_103485591.1 penicillin-binding protein 1A -
  V9L13_RS22805 (V9L13_22805) pilM 5038236..5039300 (+) 1065 WP_338800599.1 pilus assembly protein PilM Machinery gene
  V9L13_RS22810 (V9L13_22810) - 5039300..5039866 (+) 567 WP_003220894.1 PilN domain-containing protein -
  V9L13_RS22815 (V9L13_22815) pilO 5039863..5040486 (+) 624 WP_003220896.1 type 4a pilus biogenesis protein PilO -
  V9L13_RS22820 (V9L13_22820) - 5040483..5041010 (+) 528 WP_338800600.1 pilus assembly protein PilP -
  V9L13_RS22825 (V9L13_22825) pilQ 5041024..5043096 (+) 2073 WP_338800601.1 type IV pilus secretin PilQ Machinery gene
  V9L13_RS22830 (V9L13_22830) aroK 5043101..5043619 (+) 519 WP_045122260.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38105.63 Da        Isoelectric Point: 4.7373

>NTDB_id=945388 V9L13_RS22805 WP_338800599.1 5038236..5039300(+) (pilM) [Pseudomonas sp. RSB 5.4]
MLGLFNKKTNTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPPNAVVEKNIAELEGVGNALSRVLVKARTGLKSVAV
AVAGSAVITKIIEMDAGLTDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLANQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYISEVLQPFRDALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=945388 V9L13_RS22805 WP_338800599.1 5038236..5039300(+) (pilM) [Pseudomonas sp. RSB 5.4]
GTGCTGGGACTCTTCAATAAAAAGACCAATACGTTACTGGGGATCGACATCAGCTCCACTTCGGTGAAGCTGCTGGAGCT
TAGCCGCCAGGGCGACCGCTACCGGGTCGAGGCCTACGCGGTGGAACCTCTGCCGCCCAACGCCGTGGTCGAAAAGAACA
TCGCCGAGCTCGAAGGCGTGGGCAATGCCTTGTCTCGGGTACTGGTCAAGGCGCGCACCGGGCTCAAGAGCGTGGCGGTG
GCGGTGGCCGGCTCTGCGGTGATCACCAAAATCATCGAGATGGACGCCGGGCTGACCGATGACGAGCTGGAAAACCAGCT
CAAGATCGAAGCCGACCAATACATTCCCTATCCACTGGACGAAGTCGCTATCGACTTCGAAGTCCAGGGCGTTTCGCCGC
GCAACCCGGAGCGGGTCAACGTGCTGCTTGCCGCCTGTCGCAAGGAAAACGTCGAAGTGCGCGAGGCGGCGCTGGCGCTG
GCCGGGCTGACGGCGCGAGTGGTCGATGTCGAGGCCTATGCGCTGGAGCGCTCGTTCGGCCTGCTCGCCAATCAACTGGC
CGCCTCGCAGGAACGCCTGACGGTGGCCGTGGTGGACATCGGCGCGACCATGACCACTCTCAGCGTCCTGCACAATGGCC
GCATCATCTATACCCGCGAGCAACTGTTCGGTGGCCGCCAACTGACCGAGGAGATTCAGCGCCGCTACGGCCTGACCGTC
GAGCAGGCCGGGCTGGCGAAAAAGCAGGGCGGTCTGCCGGATGACTATATCAGCGAGGTTTTGCAGCCGTTTCGGGATGC
GCTGGTACAGCAGGTGTCGCGCTCGTTGCAGTTTTTCTTCGCCTCCGGGCAGTACAACGCAGTCGATCACATTCTGCTGG
CCGGTGGCACGGCGTCGGTGCCAGGGCTGGATCGCCTGATCGAGCAGCGCCTGGGCACCCCGACCCAGGTCGCCAACCCG
TTTGCCGACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCCCTGGCCAGTGACGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483