Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   V8687_RS02395 Genome accession   NZ_CP146610
Coordinates   550148..551860 (-) Length   570 a.a.
NCBI ID   WP_338726449.1    Uniprot ID   -
Organism   Shewanella baltica strain TMP1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 545148..556860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8687_RS02365 (V8687_02360) mutT 545621..546013 (-) 393 WP_012588718.1 8-oxo-dGTP diphosphatase MutT -
  V8687_RS02370 (V8687_02365) yacG 546090..546299 (-) 210 WP_012588717.1 DNA gyrase inhibitor YacG -
  V8687_RS02375 (V8687_02370) zapD 546373..547107 (-) 735 WP_006083354.1 cell division protein ZapD -
  V8687_RS02380 (V8687_02375) coaE 547100..547717 (-) 618 WP_338726446.1 dephospho-CoA kinase -
  V8687_RS02385 (V8687_02380) pilD 547757..548683 (-) 927 WP_338726447.1 A24 family peptidase Machinery gene
  V8687_RS02390 (V8687_02385) pilC 548723..549994 (-) 1272 WP_338726448.1 type II secretion system F family protein Machinery gene
  V8687_RS02395 (V8687_02390) pilB 550148..551860 (-) 1713 WP_338726449.1 type IV-A pilus assembly ATPase PilB Machinery gene
  V8687_RS02400 (V8687_02395) - 551955..552377 (-) 423 WP_338726450.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  V8687_RS02405 (V8687_02400) nadC 553058..553939 (-) 882 WP_006083348.1 carboxylating nicotinate-nucleotide diphosphorylase -
  V8687_RS02410 (V8687_02405) ampD 554210..554773 (+) 564 WP_338726451.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V8687_RS02415 (V8687_02410) ampE 554843..555694 (+) 852 WP_338726452.1 beta-lactamase regulator AmpE -
  V8687_RS02420 (V8687_02415) pdhR 555934..556686 (+) 753 WP_006083345.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62836.31 Da        Isoelectric Point: 6.2567

>NTDB_id=943351 V8687_RS02395 WP_338726449.1 550148..551860(-) (pilB) [Shewanella baltica strain TMP1]
MPTSGLHLGLSTLFIRKGLLNEEQMANAITKSRQNKHALVSTLVQSKLVSARAIAELCYEEYGTPLLDLNEFDLASIPED
FLNKKLIEKHRCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEEDKLAKALEKILEEDISGLDLGGMDEAA
LAAIEITDTDRRSEESTGESSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQAEVIPEHELLRLGF
AQAQITQGFTTFKPVGCEHCSAGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKVQGMRDLRQSGLLKVMHGVTSI
AEVNRVTSFG

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=943351 V8687_RS02395 WP_338726449.1 550148..551860(-) (pilB) [Shewanella baltica strain TMP1]
ATGCCAACATCAGGTCTTCATTTAGGTTTATCTACTTTATTTATCCGCAAAGGCCTGCTAAATGAAGAGCAAATGGCTAA
TGCCATCACAAAATCTCGGCAAAATAAACATGCCCTTGTTAGTACGTTAGTACAAAGTAAGCTGGTCTCTGCTCGCGCGA
TTGCCGAACTGTGCTATGAAGAATACGGCACACCACTGCTAGACCTTAATGAGTTTGATCTTGCCTCCATTCCTGAAGAT
TTCCTCAATAAGAAACTCATTGAAAAACATCGCTGCTTACCCTTATTTAAACGTGGAAATCGCCTCTATATTGGCACATC
CGACCCGACCAATATTGCCGCCCTTGAAGACTTCCAATTTAGTGCAGGCCTGCATGCAGAAGCGATTCTGGTTGAAGAAG
ATAAACTTGCTAAAGCGTTGGAGAAAATTCTCGAAGAAGATATTAGTGGTCTCGATTTAGGCGGCATGGACGAAGCAGCG
CTGGCAGCGATAGAAATTACTGATACAGACAGACGTAGCGAAGAGTCTACTGGTGAGAGTAGTGACGATGCGCCTATTGT
CATTTATATCAATAAAATTTTGACCGATGCCATCCGCAAAGGTGCGTCAGATTTACACTTTGAACCCTATGAGAAACGCT
ATCGCATACGGTTTCGTATAGATGGTATTCTGCACGAAGTCTCTGAACCGCCGATTAGTCTGGCTGGTCGTCTTTCTGCA
CGCTTAAAAGTGATGTCAAAACTCGATATCGCCGAACGGCGCGTGCCACAAGATGGTCGAATTAAGATGAAGCTTTCCCG
CACTAAATCTATCGACTTTCGGGTTAGCACTCTGCCGACCCTATGGGGTGAAAAGATTGTAATGCGGATTTTGGACTCGT
CCTCAGCACAACTAGGCATCGAAAAACTCGGCTACGAGCCCGACCAAGAAAAACTCTACCTTGAGATGCTGGCAAAACCC
CAAGGGATGATTTTAGTGACAGGCCCTACTGGCTCAGGTAAAACCGTCTCCCTTTACACTGGGTTAAATATTCTCAATAC
CGAAGAGCGTAATATTTCCACCGCTGAAGATCCGGTTGAGATTAACCTTGAGGGCGTGAATCAGGTTCATATTAATTTAA
AAGCAGGGCTAACCTTCGCCTCAGCACTGCGTTCGTTCTTACGCCAAGACCCAGATGTAGTGATGGTGGGCGAAATCCGC
GATCTGGAAACCGCCGAAATTGCCATTAAAGCGGCACAAACTGGCCACTTAGTCTTATCGACCTTACACACCAACTCAGC
CGCCGAAACCTTAACCCGTTTAATCAACATGGGTGTGCCTGGCTATAACATAGCTAGCTCTGTGAATTTGATTATTGCCC
AGCGCTTAGCGCGTCGTCTCTGCCCCGAATGTAAACAAGCTGAAGTGATACCCGAGCATGAATTACTGCGCCTAGGCTTT
GCCCAAGCGCAAATTACTCAGGGCTTTACCACCTTTAAGCCTGTTGGCTGTGAGCATTGCTCTGCGGGCTACAAGGGCCG
AGTCGGTATTTACGAAGTGATGAAAATGTCCGATGAAATAGCCCGCACTATCATGGAGGGTGGTAACTCATTGCAAATAG
CCTCCCAAGCTAAAGTCCAAGGAATGCGAGATTTACGGCAATCAGGGTTATTGAAAGTGATGCATGGCGTCACTAGCATT
GCAGAAGTCAATCGCGTTACAAGTTTTGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.537

98.421

0.586
  pilB Acinetobacter baumannii D1279779

57.496

99.474

0.572
  pilB Legionella pneumophila strain ERS1305867

56.228

98.596

0.554
  pilB Vibrio cholerae strain A1552

53.333

97.368

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.399

100

0.516
  pilB Vibrio campbellii strain DS40M4

51.15

99.123

0.507
  pilF Neisseria gonorrhoeae MS11

47.35

99.298

0.47
  pilF Thermus thermophilus HB27

39.046

99.298

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.71

97.895

0.379