Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSGK_RS20495 Genome accession   NZ_CP146285
Coordinates   4496636..4498711 (-) Length   691 a.a.
NCBI ID   WP_374979178.1    Uniprot ID   -
Organism   Pseudomonas solani strain GK13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4491636..4503711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSGK_RS20480 (PSGK_20365) - 4493267..4494946 (-) 1680 WP_374979177.1 SPOR domain-containing protein -
  PSGK_RS20485 (PSGK_20370) aroB 4494957..4496060 (-) 1104 WP_184489683.1 3-dehydroquinate synthase -
  PSGK_RS20490 (PSGK_20375) aroK 4496112..4496630 (-) 519 WP_081672329.1 shikimate kinase AroK -
  PSGK_RS20495 (PSGK_20380) pilQ 4496636..4498711 (-) 2076 WP_374979178.1 type IV pilus secretin PilQ Machinery gene
  PSGK_RS20500 (PSGK_20385) pilP 4498765..4499295 (-) 531 WP_374979179.1 type 4a pilus biogenesis lipoprotein PilP -
  PSGK_RS20505 (PSGK_20390) pilO 4499292..4499915 (-) 624 WP_374979180.1 type 4a pilus biogenesis protein PilO -
  PSGK_RS20510 (PSGK_20395) - 4499912..4500484 (-) 573 WP_374979181.1 PilN domain-containing protein -
  PSGK_RS20515 (PSGK_20400) pilM 4500484..4501548 (-) 1065 WP_021221345.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74021.09 Da        Isoelectric Point: 4.8305

>NTDB_id=942494 PSGK_RS20495 WP_374979178.1 4496636..4498711(-) (pilQ) [Pseudomonas solani strain GK13]
MNSSLSRLGISLLAAMLSPVLLAADLQGLDVAAMPGGKVELKLAFDEPIAAPRGYTIEQPARIALDLPGVANKLGSKNRE
LGVGNARSVTIVEAKDRTRLIVNLATLVPYSTRVEGNNLFVVVGEAAIAGQQVASAAPVAAPVAAAPVKAFAAVGKAISN
IDFQRGDQGEGNVVITLSDPTVSPDIQEQGGKIRLDFSKTQLPESLRVRLDVKDFATPVQFVSASGSSGNASITIEPTGY
YDYLAYQTENKLTVSIKPLTQDDVEKRKNERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKAADISKLFQSVTSGNES
SDERGSITVDDRTNSIIAYQTQDKLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVNWRGARVGDNNFVIGGEGG
TRTGGGSTSPNAALGTFVDMGATGATSSIGIGFVTDNTILDLELSAMEKTGNGEIVSQPKVVTSDKETAKILKGAEIPYQ
EASSSGASTTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFSNALNGVPPINKNEVNAKVLVSDGETIVIGGVFSNT
QTKATDKVPFLGDLPFLGRMFKRDIVQDKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=942494 PSGK_RS20495 WP_374979178.1 4496636..4498711(-) (pilQ) [Pseudomonas solani strain GK13]
ATGAATAGCTCGCTTTCGCGCCTCGGTATTTCTCTATTGGCCGCCATGTTGTCGCCAGTACTGTTGGCCGCTGACCTGCA
GGGCCTCGATGTAGCGGCTATGCCTGGTGGCAAGGTCGAACTGAAACTCGCTTTTGACGAGCCCATTGCGGCGCCGCGTG
GCTATACCATTGAGCAGCCAGCGCGCATTGCTCTGGATCTTCCCGGGGTAGCCAACAAGCTGGGCAGCAAGAATCGTGAG
CTCGGCGTCGGTAACGCGCGTAGCGTCACTATCGTCGAAGCGAAAGACCGTACCCGGTTGATCGTCAATCTAGCGACACT
CGTGCCTTACAGTACGCGTGTTGAGGGTAACAATCTCTTCGTTGTAGTCGGTGAAGCAGCTATTGCCGGTCAGCAAGTTG
CCAGTGCTGCTCCTGTGGCAGCTCCCGTCGCAGCTGCGCCCGTCAAGGCTTTTGCGGCGGTCGGCAAGGCAATCAGCAAT
ATCGACTTCCAGCGCGGCGATCAGGGCGAGGGCAACGTTGTCATCACGCTGTCCGACCCGACTGTGAGCCCGGATATTCA
GGAGCAGGGTGGCAAGATCCGCCTCGACTTCTCCAAGACCCAGCTTCCTGAGTCGCTGCGTGTCCGTCTTGATGTGAAGG
ACTTTGCGACCCCTGTTCAATTCGTCAGTGCCTCTGGCTCCTCGGGGAACGCCAGCATCACCATCGAGCCCACTGGCTAT
TACGACTACCTGGCGTATCAGACCGAGAACAAGCTCACCGTCAGCATCAAGCCGTTGACGCAGGATGATGTTGAGAAGCG
CAAGAACGAACGCTTTGCCTACTCCGGTGAGAAGCTGTCGCTGAACTTTCAGGACATCGATGTACGTTCGGTGTTGCAGT
TGATCGCCGACTTCACCGACCTCAACCTGGTGGCCTCGGATACCGTTCAAGGCAATATCACGCTGCGCCTGCAGAATGTG
CCCTGGGACCAGGCGTTGGACCTTGTCCTGAAAACCAAGGGGCTCGACAAGCGCAAGGTCGGTAACGTGCTGCTGGTCGC
TCCGGCAGACGAGATTGCCGCGCGCGAGCGCCAGGAACTCGAGTCTCAGAAACAGATCGCCGAGCTGGCGCCTCTGCGCC
GTGAGCTGATCCAGGTGAACTACGCCAAGGCGGCAGATATCTCCAAGCTATTCCAGTCCGTGACCAGTGGCAACGAGTCC
TCGGATGAGCGTGGCTCTATCACAGTGGATGACCGTACCAACAGCATTATTGCCTACCAGACCCAGGACAAGCTGGACGA
ACTGCGCCGTATCGTTGCGCAGCTCGATATCCCGGTTCGTCAGGTCATGATCGAGGCTCGCATCGTTGAAGCGAACGTCG
ACTACGACAAATCGTTGGGTGTGAACTGGCGTGGTGCTCGTGTCGGTGACAACAACTTCGTTATCGGTGGCGAGGGCGGC
ACTCGTACCGGCGGTGGTAGCACAAGCCCCAACGCCGCTCTTGGTACCTTCGTTGATATGGGGGCTACCGGTGCGACTTC
CTCTATCGGCATTGGTTTCGTAACCGATAACACCATTCTCGACCTCGAACTCTCCGCCATGGAGAAGACCGGTAACGGCG
AGATCGTGTCCCAGCCGAAGGTTGTAACCTCCGACAAGGAGACTGCGAAGATCCTGAAGGGCGCTGAAATCCCTTACCAG
GAAGCCAGCTCCAGCGGTGCCAGCACCACCTCCTTCAAGGAAGCTGCTTTGTCCCTGGAAGTGACCCCGCAGATCACGCC
TGACAACCGCATCATCATGGAAGTCAAGGTGACCAAGGATGCCCCGGACTTCTCCAACGCGTTGAATGGCGTACCGCCGA
TCAACAAGAACGAGGTCAACGCCAAGGTTCTGGTCAGCGATGGCGAAACCATTGTGATCGGTGGTGTGTTCTCCAACACC
CAGACCAAGGCGACCGACAAGGTTCCGTTCCTCGGTGACCTGCCGTTCCTCGGCCGCATGTTCAAGCGTGATATCGTTCA
GGACAAGAAATCCGAGCTGCTAGTCTTCCTCACCCCTCGTATCATGAACAACCAGGCTATTGCGGTGAGTCGCTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

AMIN domain (AMIN)

(40-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

Bacterial type II and III secretion system protein (Secretin)

(526-682)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

77.622

100

0.803
  comQ Acinetobacter baylyi ADP1

42.919

100

0.43
  pilQ Acinetobacter baumannii strain A118

40.942

100

0.415
  pilQ Acinetobacter baumannii D1279779

40.942

100

0.415
  pilQ Legionella pneumophila strain ERS1305867

37.683

98.698

0.372
  pilQ Legionella pneumophila strain Lp02

37.683

98.698

0.372