Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PSGK_RS20515 Genome accession   NZ_CP146285
Coordinates   4500484..4501548 (-) Length   354 a.a.
NCBI ID   WP_021221345.1    Uniprot ID   A0AAU7Y112
Organism   Pseudomonas solani strain GK13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4495484..4506548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSGK_RS20490 (PSGK_20375) aroK 4496112..4496630 (-) 519 WP_081672329.1 shikimate kinase AroK -
  PSGK_RS20495 (PSGK_20380) pilQ 4496636..4498711 (-) 2076 WP_374979178.1 type IV pilus secretin PilQ Machinery gene
  PSGK_RS20500 (PSGK_20385) pilP 4498765..4499295 (-) 531 WP_374979179.1 type 4a pilus biogenesis lipoprotein PilP -
  PSGK_RS20505 (PSGK_20390) pilO 4499292..4499915 (-) 624 WP_374979180.1 type 4a pilus biogenesis protein PilO -
  PSGK_RS20510 (PSGK_20395) - 4499912..4500484 (-) 573 WP_374979181.1 PilN domain-containing protein -
  PSGK_RS20515 (PSGK_20400) pilM 4500484..4501548 (-) 1065 WP_021221345.1 pilus assembly protein PilM Machinery gene
  PSGK_RS20520 (PSGK_20405) - 4501733..4504153 (+) 2421 WP_374981960.1 penicillin-binding protein 1A -
  PSGK_RS20525 (PSGK_20410) - 4504269..4505537 (-) 1269 WP_271659255.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37767.98 Da        Isoelectric Point: 4.4899

>NTDB_id=942495 PSGK_RS20515 WP_021221345.1 4500484..4501548(-) (pilM) [Pseudomonas solani strain GK13]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLSKAKASSKSVAV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGVSSRNSERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLAAQLGSGHDELTVAVVDIGATMTTLSVLHNGRTIYSREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTQVANP
FAEMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=942495 PSGK_RS20515 WP_021221345.1 4500484..4501548(-) (pilM) [Pseudomonas solani strain GK13]
GTGCTAGGGCTCTTCAACAAGAAAGCGAATACGCTGCTGGGGATCGACATAAGCTCGACCTCAGTCAAGCTCCTTGAATT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCCTACGCCGTCGAGCCGCTCCCACCGAATGCTGTGGTCGAAAAGAACA
TCGCCGAACTGGAGGGGGTCGGCCAGGCGTTGTCTCGCGTTCTGAGCAAGGCGAAAGCCAGCTCAAAATCCGTGGCGGTT
GCCGTCGCGGGCTCCGCTGTGATCACCAAGACCATCGAGATGGATGCCGGTCTTTCCGACGACGAGTTGGAAAACCAGCT
GAAGATCGAGGCGGACCAGTACATCCCTTACCCGCTCGAAGAAGTCGCCATCGACTTCGAGGTGCAGGGTGTGTCTTCGC
GTAACTCCGAGCGGGTTGATGTGCTGCTTGCGGCATGCCGCAAGGAGAACGTCGAAGTCCGAGAGGCGGCACTGGCACTG
GCAGGCCTGACCGCCAAGGTCGTCGATGTCGAAGCCTATGCGCTGGAACGTGCCTATGGGCTGCTCGCTGCGCAGCTGGG
CAGTGGCCATGACGAGCTGACTGTCGCGGTAGTGGATATCGGCGCGACCATGACGACGCTGAGCGTTCTGCACAATGGGC
GGACCATCTACAGCCGCGAGCAGCTTTTTGGCGGGCGTCAGCTCACCGAGGAGATTCAGCGACGCTATGGCCTGTCCGTG
GAAGAGGCTGGTCTTGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTGTTGCAGCCGTTCAAGGATGC
AGTGGTCCAGCAAGTGTCTCGCTCGCTGCAGTTCTTCTTCGCTGCGGGTCAGTTCAACGATGTCGACTACATCCTCCTGG
CGGGCGGTACTGCGTCCATTCCTGATCTGGATCGGCTGATCCAGCAGAAGATCGGCACTCCTACCCAGGTTGCCAACCCC
TTCGCGGAGATGGCGCTGAGCGGCAAGGTCAATGCGGGTGCATTGGCCAGTGATGCCCCGGCGCTGATGATTGCCTGCGG
ACTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

48.451

100

0.486