Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   V4941_RS08545 Genome accession   NZ_CP144736
Coordinates   1970232..1971449 (+) Length   405 a.a.
NCBI ID   WP_012699853.1    Uniprot ID   M9YCM4
Organism   Azotobacter vinelandii DJ     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1965232..1976449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4941_RS08530 (V4941_08525) nadC 1965775..1966623 (+) 849 WP_012699858.1 carboxylating nicotinate-nucleotide diphosphorylase -
  V4941_RS08535 (V4941_08530) - 1967281..1967778 (-) 498 WP_012699856.1 pilin -
  V4941_RS08540 (V4941_08535) pilB 1968307..1970007 (+) 1701 WP_012699854.1 type IV-A pilus assembly ATPase PilB Machinery gene
  V4941_RS08545 (V4941_08540) pilC 1970232..1971449 (+) 1218 WP_012699853.1 type II secretion system F family protein Machinery gene
  V4941_RS08550 (V4941_08545) pilD 1971452..1972321 (+) 870 WP_012699852.1 A24 family peptidase Machinery gene
  V4941_RS08555 (V4941_08550) coaE 1972483..1973091 (+) 609 WP_012699851.1 dephospho-CoA kinase -
  V4941_RS08560 (V4941_08555) yacG 1973088..1973288 (+) 201 WP_041806982.1 DNA gyrase inhibitor YacG -
  V4941_RS08565 (V4941_08560) - 1973394..1974080 (-) 687 WP_012699850.1 energy-coupling factor ABC transporter permease -
  V4941_RS08570 (V4941_08565) - 1974077..1974544 (-) 468 WP_012699849.1 hypothetical protein -
  V4941_RS08575 (V4941_08570) - 1974541..1974966 (-) 426 WP_012699848.1 GNAT family N-acetyltransferase -
  V4941_RS08580 (V4941_08575) - 1975271..1975444 (+) 174 WP_012699847.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44210.02 Da        Isoelectric Point: 10.0225

>NTDB_id=934684 V4941_RS08545 WP_012699853.1 1970232..1971449(+) (pilC) [Azotobacter vinelandii DJ]
MAEKALKISLFTWEGIDRRGARIKGELNGRNPALVKAQLRKQGINPTRVRKKAGSLFGGDQKIKPLDIALFTRQMATMMK
AGVPLLQAFDIISEGFDKPAMRKLVDEVKQEVAAGNGFAASLRKKPRYFDNLYCNLVESGEQSGALESLLERIATYKEKT
EALKARIKKAMTYPIAVILVAIAVSAILLIKVVPQFQSVFANFGAELPALTLLVVNLSEVLQEYWFYALLGIPIAVLILK
QAHRRSEAFRNWTDRSLLKLPIVGQILYKSAVARFARTLSTTFAAGVPLVDALDSVAGATGNVVFRNATEKVKADVTTGM
QLNFSMRTTGTFPSMAIQMTAIGEESGTLDEMLDKVAGFYEAEVDNMVDSLTGLLEPMIMAVLGVLVGGLIVAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=934684 V4941_RS08545 WP_012699853.1 1970232..1971449(+) (pilC) [Azotobacter vinelandii DJ]
ATGGCGGAAAAAGCGTTGAAAATCAGTCTGTTCACCTGGGAGGGCATCGACCGGCGCGGTGCCAGGATCAAGGGAGAACT
GAACGGGAGAAATCCGGCGCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCAATCCGACCAGGGTACGCAAGAAGGCCG
GCTCGCTGTTCGGCGGCGACCAGAAGATCAAACCGCTGGATATCGCCCTGTTCACCCGGCAGATGGCCACCATGATGAAG
GCAGGCGTGCCGCTGCTGCAGGCCTTCGACATCATCTCCGAGGGTTTCGACAAACCGGCCATGCGCAAGCTGGTGGACGA
AGTGAAACAGGAGGTGGCGGCGGGCAACGGTTTCGCCGCTTCACTGCGCAAGAAGCCCCGCTATTTCGACAACCTCTACT
GCAACCTGGTGGAGTCCGGCGAGCAGTCCGGCGCCCTGGAAAGCCTGCTGGAGCGGATCGCCACCTACAAGGAGAAGACC
GAGGCGCTGAAGGCCAGGATCAAGAAGGCGATGACCTACCCCATCGCGGTGATCCTGGTGGCGATCGCCGTCTCGGCCAT
TCTGCTGATAAAGGTGGTACCGCAATTCCAGTCGGTGTTCGCCAACTTCGGTGCCGAACTGCCGGCGCTCACGCTGCTGG
TCGTCAACCTGTCGGAAGTTCTGCAGGAATACTGGTTCTACGCGCTGCTCGGAATACCCATCGCAGTTCTCATCCTGAAG
CAGGCCCACCGGCGCTCCGAGGCGTTTCGCAACTGGACGGATCGCAGCCTGCTGAAGCTGCCGATCGTCGGCCAAATCCT
CTACAAGTCGGCGGTGGCCCGCTTCGCCCGCACCCTGTCCACCACTTTCGCCGCCGGTGTGCCGCTGGTCGACGCACTCG
ACTCGGTGGCCGGCGCCACCGGCAACGTGGTGTTCCGCAACGCCACCGAGAAGGTCAAGGCCGACGTCACCACCGGCATG
CAACTGAACTTCTCCATGCGCACCACCGGCACCTTCCCCAGCATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
CACGCTGGACGAGATGCTCGACAAGGTGGCGGGCTTCTACGAGGCCGAAGTGGACAACATGGTGGACAGCCTGACCGGTT
TGCTGGAGCCGATGATCATGGCGGTGCTCGGCGTGCTGGTCGGCGGCCTGATCGTCGCCATGTACCTGCCGATCTTCCAG
TTGGGCTCCGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M9YCM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.259

100

0.793
  pilC Acinetobacter baylyi ADP1

59.852

100

0.6
  pilC Acinetobacter baumannii D1279779

58.519

100

0.585
  pilC Legionella pneumophila strain ERS1305867

52.525

97.778

0.514
  pilG Neisseria meningitidis 44/76-A

43.719

98.272

0.43
  pilG Neisseria gonorrhoeae MS11

43.719

98.272

0.43
  pilC Vibrio cholerae strain A1552

42.716

100

0.427
  pilC Vibrio campbellii strain DS40M4

41.96

98.272

0.412