Detailed information NTG0930357

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P9963_RS02410
• Genome accession: NZ_CP143649
• Coordinates: 512797..513945 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain CPO506
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 507797..518945

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P9963_RS02385 (P9963_002385)	-	508948..509244 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
P9963_RS02390 (P9963_002390)	-	509241..509834 (-)	594	WP_003084544.1	YggT family protein	-
P9963_RS02395 (P9963_002395)	proC	509845..510666 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
P9963_RS02400 (P9963_002400)	-	510678..511370 (-)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
P9963_RS02405 (P9963_002405)	pilT	511585..512619 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
P9963_RS02410 (P9963_002410)	pilU	512797..513945 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
P9963_RS02415 (P9963_002415)	czcD	513953..514852 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
P9963_RS02420 (P9963_002420)	-	515006..515410 (+)	405	WP_003084560.1	NINE protein	-
P9963_RS02425 (P9963_002425)	-	515653..517026 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
P9963_RS02430 (P9963_002430)	-	517023..518207 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=930357 P9963_RS02410 WP_003100959.1 512797..513945(+) (pilU) [Pseudomonas aeruginosa strain CPO506]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=930357 P9963_RS02410 WP_003100959.1 512797..513945(+) (pilU) [Pseudomonas aeruginosa strain CPO506]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382