Detailed information NTG0929682

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: V1M48_RS12860
• Genome accession: NZ_CP143559
• Coordinates: 597804..598673 (-)
• Length: 289 a.a.
• NCBI ID: WP_005446255.1
• Uniprot ID: A0A3A1Q6Q3
• Organism: Vibrio harveyi strain WHSS0915
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 592804..603673

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V1M48_RS12825 (V1M48_12825)	rpsP	593806..594054 (+)	249	WP_004410028.1	30S ribosomal protein S16	-
V1M48_RS12830 (V1M48_12830)	rimM	594083..594631 (+)	549	WP_005446245.1	ribosome maturation factor RimM	-
V1M48_RS12835 (V1M48_12835)	trmD	594659..595402 (+)	744	WP_005446247.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
V1M48_RS12840 (V1M48_12840)	rplS	595444..595797 (+)	354	WP_005379971.1	50S ribosomal protein L19	-
V1M48_RS12845 (V1M48_12845)	yacG	596164..596358 (-)	195	WP_005446249.1	DNA gyrase inhibitor YacG	-
V1M48_RS12850 (V1M48_12850)	zapD	596421..597161 (-)	741	WP_005446251.1	cell division protein ZapD	-
V1M48_RS12855 (V1M48_12855)	coaE	597189..597803 (-)	615	WP_017189130.1	dephospho-CoA kinase	-
V1M48_RS12860 (V1M48_12860)	pilD	597804..598673 (-)	870	WP_005446255.1	prepilin peptidase	Machinery gene
V1M48_RS12865 (V1M48_12865)	pilC	598746..599969 (-)	1224	WP_258455382.1	type II secretion system F family protein	Machinery gene
V1M48_RS12870 (V1M48_12870)	pilB	600003..601688 (-)	1686	WP_005446257.1	type IV-A pilus assembly ATPase PilB	Machinery gene
V1M48_RS12875 (V1M48_12875)	pilA	601688..602116 (-)	429	WP_009698754.1	type IV pilin protein	Machinery gene
V1M48_RS12880 (V1M48_12880)	nadC	602381..603268 (-)	888	WP_029790346.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32536.74 Da
• Isoelectric Point: 7.5422

>NTDB_id=929682 V1M48_RS12860 WP_005446255.1 597804..598673(-) (pilD) [Vibrio harveyi strain WHSS0915]
MEVFQYYPWLFVVFTAIFGLIVGSFLNVVIYRLPKIMELEWRRECAESFPEYKIEPPKETLTLSVPRSSCQQCGTHIRIR
DNIPVISWLLLKGKCHNCQSPISARYPLIELLTAFCSGFIAFHFGFSYFTVALVFFTFVLIAATFIDLDTMLLPDQLTLP
LMWAGIALALAGISPVSLQDSIIGAMAGYLCLWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPMIILLSSVVGV
IFGLIQLRLQKQGIEKAFPFGPYLAIAGWVSLIWGNQILNWYFTSILGV

Nucleotide

• Length: 870 bp

>NTDB_id=929682 V1M48_RS12860 WP_005446255.1 597804..598673(-) (pilD) [Vibrio harveyi strain WHSS0915]
ATGGAAGTATTCCAGTACTACCCATGGCTATTCGTTGTATTTACCGCTATTTTTGGTTTGATCGTGGGTAGTTTCCTCAA
CGTAGTTATTTACCGTTTACCTAAGATCATGGAACTAGAATGGCGACGCGAGTGTGCTGAATCATTCCCTGAATACAAGA
TCGAGCCACCAAAAGAGACATTAACCTTAAGCGTCCCACGTTCATCTTGTCAGCAATGCGGTACACATATCCGCATTAGA
GACAATATCCCTGTGATTAGTTGGCTATTACTAAAAGGTAAATGCCACAACTGCCAATCACCTATCAGTGCCCGTTACCC
TCTTATCGAACTGCTTACCGCTTTTTGTTCGGGTTTTATCGCTTTTCATTTCGGTTTTAGCTACTTCACTGTGGCGCTGG
TTTTCTTTACCTTTGTATTGATTGCTGCCACATTTATCGACCTTGATACCATGTTGCTGCCAGACCAACTCACACTACCA
TTGATGTGGGCGGGTATTGCTCTTGCTCTTGCCGGTATCAGCCCTGTGAGTCTTCAAGATTCTATTATTGGAGCAATGGC
AGGCTACTTATGTCTTTGGAGTGTTTATTGGTTATTCAAACTCCTCACAGGCAAAGAGGGAATGGGCTATGGCGACTTTA
AACTCCTTGCTGCATTAGGAGCTTGGTTGGGCTGGCAATCTCTGCCGATGATCATTTTGCTTTCTTCGGTAGTAGGCGTC
ATTTTTGGTCTTATCCAACTGCGCTTGCAAAAGCAAGGTATCGAAAAAGCCTTTCCTTTTGGTCCTTACCTTGCAATTGC
AGGATGGGTGAGCCTAATTTGGGGCAATCAAATCCTCAACTGGTACTTTACGTCGATTCTAGGAGTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: A0A3A1Q6Q3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	97.232	100	0.972
pilD	Vibrio cholerae strain A1552	74.306	99.654	0.74
pilD	Acinetobacter baumannii D1279779	52.768	93.772	0.495
pilD	Acinetobacter nosocomialis M2	52.399	93.772	0.491
pilD	Neisseria gonorrhoeae MS11	45.652	95.502	0.436