Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VOI35_RS03725 Genome accession   NZ_CP142685
Coordinates   789516..791180 (-) Length   554 a.a.
NCBI ID   WP_407276787.1    Uniprot ID   -
Organism   Halothiobacillus sp. DCM-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 784516..796180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VOI35_RS03700 (VOI35_03690) - 785231..786211 (-) 981 WP_407276543.1 YgfZ/GcvT domain-containing protein -
  VOI35_RS03705 (VOI35_03695) - 786308..786724 (+) 417 WP_407276544.1 hypothetical protein -
  VOI35_RS03710 (VOI35_03700) coaE 786752..787375 (-) 624 WP_407276545.1 dephospho-CoA kinase -
  VOI35_RS03715 (VOI35_03705) pilD 787372..788256 (-) 885 WP_407276546.1 prepilin peptidase Machinery gene
  VOI35_RS03720 (VOI35_03710) pilC 788266..789504 (-) 1239 WP_407276547.1 type II secretion system F family protein Machinery gene
  VOI35_RS03725 (VOI35_03715) pilB 789516..791180 (-) 1665 WP_407276787.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VOI35_RS03730 (VOI35_03720) - 791283..791702 (-) 420 WP_407276548.1 PAS domain-containing protein -
  VOI35_RS03735 (VOI35_03725) - 791763..792668 (-) 906 WP_407276549.1 MerR family transcriptional regulator -
  VOI35_RS03740 (VOI35_03730) - 792782..793576 (+) 795 WP_407276550.1 SDR family NAD(P)-dependent oxidoreductase -
  VOI35_RS03745 (VOI35_03735) - 793573..794007 (+) 435 WP_407276551.1 nuclear transport factor 2 family protein -
  VOI35_RS03750 (VOI35_03740) - 794182..795735 (+) 1554 WP_407276552.1 2-isopropylmalate synthase -
  VOI35_RS03755 (VOI35_03745) - 795743..796144 (+) 402 WP_407276553.1 hypothetical protein -

Sequence

Protein


Download         Length: 554 a.a.        Molecular weight: 60863.03 Da        Isoelectric Point: 6.1305

>NTDB_id=926414 VOI35_RS03725 WP_407276787.1 789516..791180(-) (pilB) [Halothiobacillus sp. DCM-1]
MEALVKTQTIEPQRAAELIERAKKEQEPLLQTLMSSGISGASIARATSQRFGVPLLELDAFDTTDLPLSLVPAKLLESGT
TLPLRKRGRRLTVALADPSDQGVIENLRFSTNLAIDVVTADPGKIARLLEEARNSAAAGLDDLDDDLNNIQFETEGTPDE
KSDDPLQDDIVVKFVTKTLTDAIRRKASDIHLEPYEKLFRIRFRIDGILTEIPHPPKALADRIIARVKVMSRMDISERRV
PQDGRIKLKLSNTRSIDFRVNTCPTLFGEKVVMRILDPTSAQLGIDMLGYEPDQKELYLKALNRPYGMILVTGPTGSGKT
VSLYTGLNILNTLDRNISTAEDPAEIVVPGINQVNVNAKVGLTFASALRAFLRQDPDVIMVGEIRDLETAEIAIKAAQTG
HLVLSTLHTNDAPQTLTRLVNMGVPAYNIASSVNLIIAQRLARRLCNNCKKVDHVPDEELAREGFTPVDIQSGIKIFKAV
GCDQCTGGYKGRVGLYQVMPVSEEMGRIIMRGGNAMELADQAQKEGVSDLRQSGLRKVKAGMISLEELNRVTKD

Nucleotide


Download         Length: 1665 bp        

>NTDB_id=926414 VOI35_RS03725 WP_407276787.1 789516..791180(-) (pilB) [Halothiobacillus sp. DCM-1]
ATGGAAGCTCTGGTCAAAACCCAGACCATCGAGCCGCAACGCGCAGCCGAACTGATCGAACGCGCCAAAAAAGAGCAGGA
ACCCCTGCTGCAAACCCTGATGAGCAGCGGCATTTCCGGTGCCAGCATCGCGCGGGCAACTTCTCAGCGCTTTGGCGTGC
CGCTGCTGGAGTTGGACGCCTTCGACACCACGGATCTCCCGCTGAGCCTCGTGCCGGCGAAGCTGCTGGAAAGCGGTACC
ACGCTCCCACTGCGCAAGCGCGGTAGACGCCTCACGGTCGCGCTGGCCGATCCCTCTGACCAGGGCGTGATCGAAAATCT
TCGTTTCAGCACCAATCTGGCCATTGATGTGGTCACGGCAGATCCCGGCAAGATCGCCCGCCTGCTCGAAGAAGCGCGCA
ACAGCGCCGCAGCGGGTTTAGACGATCTCGACGATGACCTGAACAATATTCAGTTTGAAACCGAGGGCACCCCCGATGAA
AAGAGCGACGACCCGCTACAGGATGACATCGTCGTCAAATTCGTCACCAAAACCCTGACCGACGCCATTCGGCGTAAAGC
ATCCGACATCCATCTGGAGCCCTATGAAAAACTGTTCCGGATCCGTTTTCGGATTGATGGCATTCTGACCGAAATCCCGC
ATCCCCCCAAGGCGTTGGCGGATCGCATCATTGCGCGCGTCAAGGTCATGAGCCGGATGGATATTTCCGAACGGCGCGTC
CCACAGGATGGGCGGATCAAACTCAAACTGTCCAATACCCGTTCTATCGATTTCCGCGTCAACACCTGCCCGACCCTGTT
TGGCGAAAAGGTCGTGATGCGGATTCTCGATCCCACCAGCGCCCAGCTCGGGATTGACATGCTGGGCTATGAGCCGGATC
AGAAAGAGCTGTACCTCAAGGCACTCAATCGCCCTTACGGCATGATTCTGGTCACCGGCCCCACGGGCTCGGGGAAAACC
GTCTCGCTGTATACCGGCCTGAACATTCTGAACACCCTGGATCGCAACATCTCCACCGCAGAGGATCCCGCGGAAATCGT
GGTACCCGGCATCAATCAGGTCAATGTCAACGCCAAGGTAGGCTTAACCTTTGCCAGCGCGCTGCGTGCCTTCCTGCGGC
AGGACCCGGATGTCATCATGGTCGGCGAGATCCGCGATCTGGAAACCGCTGAAATTGCGATCAAAGCCGCACAAACCGGC
CACTTGGTACTCTCCACCTTACATACCAATGATGCCCCGCAAACCCTGACGCGCCTCGTGAACATGGGGGTGCCCGCGTA
CAACATCGCATCCTCTGTAAACCTGATCATCGCGCAACGCCTGGCACGCCGCTTATGCAACAACTGCAAAAAAGTGGATC
ATGTGCCCGATGAAGAGTTGGCACGCGAAGGTTTTACCCCGGTCGACATCCAGAGCGGCATCAAGATATTCAAGGCCGTC
GGCTGCGATCAGTGTACCGGCGGTTATAAAGGGCGGGTCGGTCTTTACCAGGTGATGCCCGTTTCCGAGGAAATGGGGCG
TATCATCATGCGCGGAGGCAACGCCATGGAACTGGCCGATCAAGCACAAAAGGAAGGCGTTAGTGACCTACGACAATCCG
GCCTGCGCAAAGTCAAGGCCGGCATGATCAGTCTCGAAGAACTCAATCGGGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-135)

Type II/IV secretion system protein (T2SSE)

(177-444)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.122

100

0.545
  pilB Legionella pneumophila strain ERS1305867

53.832

100

0.545
  pilB Acinetobacter baumannii D1279779

54.945

98.556

0.542
  pilB Vibrio cholerae strain A1552

48.936

100

0.498
  pilF Neisseria gonorrhoeae MS11

49.55

100

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

49.077

97.834

0.48
  pilB Vibrio campbellii strain DS40M4

47.993

98.917

0.475
  pilF Thermus thermophilus HB27

38.889

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.924

94.946

0.379