Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   VOI35_RS03715 Genome accession   NZ_CP142685
Coordinates   787372..788256 (-) Length   294 a.a.
NCBI ID   WP_407276546.1    Uniprot ID   -
Organism   Halothiobacillus sp. DCM-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 782372..793256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VOI35_RS03695 (VOI35_03685) - 783941..785164 (+) 1224 WP_407276542.1 hypothetical protein -
  VOI35_RS03700 (VOI35_03690) - 785231..786211 (-) 981 WP_407276543.1 YgfZ/GcvT domain-containing protein -
  VOI35_RS03705 (VOI35_03695) - 786308..786724 (+) 417 WP_407276544.1 hypothetical protein -
  VOI35_RS03710 (VOI35_03700) coaE 786752..787375 (-) 624 WP_407276545.1 dephospho-CoA kinase -
  VOI35_RS03715 (VOI35_03705) pilD 787372..788256 (-) 885 WP_407276546.1 prepilin peptidase Machinery gene
  VOI35_RS03720 (VOI35_03710) pilC 788266..789504 (-) 1239 WP_407276547.1 type II secretion system F family protein Machinery gene
  VOI35_RS03725 (VOI35_03715) pilB 789516..791180 (-) 1665 WP_407276787.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VOI35_RS03730 (VOI35_03720) - 791283..791702 (-) 420 WP_407276548.1 PAS domain-containing protein -
  VOI35_RS03735 (VOI35_03725) - 791763..792668 (-) 906 WP_407276549.1 MerR family transcriptional regulator -

Sequence

Protein


Download         Length: 294 a.a.        Molecular weight: 31845.88 Da        Isoelectric Point: 8.3484

>NTDB_id=926412 VOI35_RS03715 WP_407276546.1 787372..788256(-) (pilD) [Halothiobacillus sp. DCM-1]
MAGFDALVDAFRQNAGLWIGFSLILGLLIGSFLNVVIHRLPIMLERGWQRDCRELLGQTPETSAPVYNLITPRSACPQCH
TAIKAWQNIPVLSWLWLRGRCAACGHPISLQYPLVELASGLLTALAAWHFGVSAFTLAVFVFGWLLLAAALIDLKTTLLP
DLLTLPLMWLGLLLAAFGLNPSVSLHDAVLGAAGGYLALWSVYWLFKLTTGKDGMGYGDFKLLAALGAWLGWQSLAMILL
LSAGVGAVIGVGMILLLRHDRRIPIPFGPYLAGAGFLALYLGDLLSQRLWGWAG

Nucleotide


Download         Length: 885 bp        

>NTDB_id=926412 VOI35_RS03715 WP_407276546.1 787372..788256(-) (pilD) [Halothiobacillus sp. DCM-1]
ATGGCCGGCTTCGATGCCTTAGTGGATGCCTTTCGGCAAAATGCCGGCCTGTGGATCGGGTTCTCCCTGATCCTGGGGCT
GCTGATTGGCAGTTTCCTGAATGTCGTCATTCATCGCCTGCCCATCATGCTGGAGCGCGGCTGGCAGCGCGACTGCCGGG
AACTGCTCGGACAAACCCCGGAGACTTCAGCACCCGTCTATAACCTGATCACTCCGCGCTCGGCCTGCCCGCAATGCCAC
ACGGCCATCAAAGCCTGGCAGAACATTCCCGTCCTGAGCTGGTTATGGCTGCGGGGTCGATGCGCGGCCTGTGGGCACCC
CATCAGTCTCCAGTATCCGCTGGTCGAACTCGCCAGTGGGTTATTGACCGCGCTCGCCGCCTGGCACTTTGGCGTGTCTG
CATTCACCTTGGCGGTGTTCGTCTTCGGCTGGCTGTTGCTGGCTGCCGCCCTCATCGACCTGAAAACCACCCTGCTCCCC
GATCTGCTCACCCTGCCCCTGATGTGGCTCGGGCTCCTGCTCGCGGCGTTCGGCCTGAATCCCAGCGTTTCGCTGCACGA
TGCGGTGCTCGGTGCAGCCGGCGGCTATCTGGCGCTGTGGAGCGTGTACTGGCTGTTCAAACTCACGACCGGCAAAGACG
GCATGGGATACGGTGATTTCAAGCTGCTGGCGGCGCTCGGCGCCTGGTTAGGCTGGCAATCCCTGGCCATGATCCTCCTG
CTCTCGGCGGGGGTCGGCGCCGTGATCGGGGTCGGCATGATCCTACTGCTGCGCCACGACCGGCGCATTCCCATTCCCTT
CGGCCCCTATCTCGCCGGGGCGGGGTTCCTGGCGCTCTATCTGGGCGACTTGCTCAGCCAGCGTCTTTGGGGATGGGCGG
GATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(141-251)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(24-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

53.069

94.218

0.5
  pilD Vibrio cholerae strain A1552

53.612

89.456

0.48
  pilD Acinetobacter nosocomialis M2

48.029

94.898

0.456
  pilD Acinetobacter baumannii D1279779

48.029

94.898

0.456
  pilD Neisseria gonorrhoeae MS11

45.802

89.116

0.408