Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   VKP53_RS00800 Genome accession   NZ_CP142354
Coordinates   119774..120208 (+) Length   144 a.a.
NCBI ID   WP_002992738.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 6745-99     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 114774..125208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VKP53_RS00770 (VKP53_00770) - 116458..116823 (+) 366 WP_002986560.1 DUF1033 family protein -
  VKP53_RS00775 (VKP53_00775) comYA 116916..117839 (+) 924 WP_136281985.1 competence type IV pilus ATPase ComGA Machinery gene
  VKP53_RS00780 (VKP53_00780) comYB 117775..118809 (+) 1035 WP_231871599.1 competence type IV pilus assembly protein ComGB Machinery gene
  VKP53_RS00785 (VKP53_00785) comYC 118811..119137 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  VKP53_RS00790 (VKP53_00790) comGD 119112..119540 (+) 429 WP_023605232.1 competence type IV pilus minor pilin ComGD -
  VKP53_RS00795 (VKP53_00795) comGE 119497..119781 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  VKP53_RS00800 (VKP53_00800) comYF 119774..120208 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  VKP53_RS00805 (VKP53_00805) comGG 120192..120518 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  VKP53_RS00810 (VKP53_00810) comYH 120616..121569 (+) 954 WP_326674822.1 class I SAM-dependent methyltransferase Machinery gene
  VKP53_RS00815 (VKP53_00815) - 121628..122824 (+) 1197 WP_030126459.1 acetate kinase -
  VKP53_RS00820 (VKP53_00820) - 123010..123318 (+) 309 Protein_115 hypothetical protein -
  VKP53_RS00825 (VKP53_00825) proC 123401..124171 (-) 771 WP_002986527.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16645.27 Da        Isoelectric Point: 10.2526

>NTDB_id=925195 VKP53_RS00800 WP_002992738.1 119774..120208(+) (comYF) [Streptococcus pyogenes strain 6745-99]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=925195 VKP53_RS00800 WP_002992738.1 119774..120208(+) (comYF) [Streptococcus pyogenes strain 6745-99]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

44.776

93.056

0.417
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396