Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   VKP53_RS00775 Genome accession   NZ_CP142354
Coordinates   116916..117839 (+) Length   307 a.a.
NCBI ID   WP_136281985.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 6745-99     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 111916..122839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VKP53_RS00765 (VKP53_00765) rpoC 112665..116306 (+) 3642 WP_030126920.1 DNA-directed RNA polymerase subunit beta' -
  VKP53_RS00770 (VKP53_00770) - 116458..116823 (+) 366 WP_002986560.1 DUF1033 family protein -
  VKP53_RS00775 (VKP53_00775) comYA 116916..117839 (+) 924 WP_136281985.1 competence type IV pilus ATPase ComGA Machinery gene
  VKP53_RS00780 (VKP53_00780) comYB 117775..118809 (+) 1035 WP_231871599.1 competence type IV pilus assembly protein ComGB Machinery gene
  VKP53_RS00785 (VKP53_00785) comYC 118811..119137 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  VKP53_RS00790 (VKP53_00790) comGD 119112..119540 (+) 429 WP_023605232.1 competence type IV pilus minor pilin ComGD -
  VKP53_RS00795 (VKP53_00795) comGE 119497..119781 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  VKP53_RS00800 (VKP53_00800) comYF 119774..120208 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  VKP53_RS00805 (VKP53_00805) comGG 120192..120518 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  VKP53_RS00810 (VKP53_00810) comYH 120616..121569 (+) 954 WP_326674822.1 class I SAM-dependent methyltransferase Machinery gene
  VKP53_RS00815 (VKP53_00815) - 121628..122824 (+) 1197 WP_030126459.1 acetate kinase -

Sequence

Protein


Download         Length: 307 a.a.        Molecular weight: 34535.62 Da        Isoelectric Point: 8.2461

>NTDB_id=925192 VKP53_RS00775 WP_136281985.1 116916..117839(+) (comYA) [Streptococcus pyogenes strain 6745-99]
MVQALAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYKLSKDK
QLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISSYHQEAQVISI
EDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGVYARLL
ELGVTKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 924 bp        

>NTDB_id=925192 VKP53_RS00775 WP_136281985.1 116916..117839(+) (comYA) [Streptococcus pyogenes strain 6745-99]
ATGGTACAAGCATTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGCAGATCAATATGATCT
TTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTCTTATTAGTCACTTTA
AATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAGCTTAGTAAAGATAAG
CAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCTGCTTCATCATCAAAA
TAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAGGTTGGCGGTAGAGGGTTGTATTTATTTGCAG
GACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAGTTATCATCAAGAAGCACAGGTTATTAGTATA
GAAGATCCTGTAGAAATTAAAAATCACCAGATTTTACAATTACAAGTTAATGATGATATTGGTATGACTTATGACAATTT
GATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAACAGCAAGAGCCGTTA
TTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTTTATGCAAGATTGTTA
GAACTTGGTGTAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACTTAATGGAGGAGCATT
GATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATCAGCTTCTTGAGGCAG
GACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA140

61.415

100

0.622
  comYA Streptococcus mutans UA159

61.415

100

0.622
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.645

100

0.612
  comYA Streptococcus gordonii str. Challis substr. CH1

60.129

100

0.609
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.486

100

0.603
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.842

100

0.596
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.842

100

0.596
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.842

100

0.596
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.842

100

0.596
  comGA Lactococcus lactis subsp. cremoris KW2

50.329

99.023

0.498
  comGA Latilactobacillus sakei subsp. sakei 23K

40.569

91.531

0.371