Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OCU90_RS14455 Genome accession   NZ_AP025508
Coordinates   3276894..3278579 (+) Length   561 a.a.
NCBI ID   WP_061022518.1    Uniprot ID   -
Organism   Vibrio splendidus strain LMG 19031     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3271894..3283579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU90_RS14435 pdhR 3273387..3274148 (-) 762 WP_004735446.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  OCU90_RS14440 ampD 3274630..3275229 (-) 600 WP_061022512.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OCU90_RS14445 nadC 3275313..3276203 (+) 891 WP_061022514.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OCU90_RS14450 - 3276459..3276893 (+) 435 WP_061022516.1 pilin -
  OCU90_RS14455 pilB 3276894..3278579 (+) 1686 WP_061022518.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCU90_RS14460 pilC 3278595..3279824 (+) 1230 WP_004735441.1 type II secretion system F family protein Machinery gene
  OCU90_RS14465 pilD 3279892..3280761 (+) 870 WP_061022520.1 A24 family peptidase Machinery gene
  OCU90_RS14470 coaE 3280764..3281378 (+) 615 WP_061022521.1 dephospho-CoA kinase -
  OCU90_RS14475 zapD 3281405..3282145 (+) 741 WP_017087400.1 cell division protein ZapD -
  OCU90_RS14480 yacG 3282227..3282424 (+) 198 WP_004735515.1 DNA gyrase inhibitor YacG -
  OCU90_RS14485 rplS 3282772..3283125 (-) 354 WP_004735514.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62093.66 Da        Isoelectric Point: 4.8730

>NTDB_id=92314 OCU90_RS14455 WP_061022518.1 3276894..3278579(+) (pilB) [Vibrio splendidus strain LMG 19031]
MLTNLPTIFRQANLLSLTQEQAVAEHVQASGVSTPEALLVLDFFSGDSLASNIKTIFGLPLVQLASTDYETLCEQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVEISDDEITSIEDLSQDDSPVSRFINQILVDAARKGASDIHFEPYEENYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLCGINQVQVSPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLSHMGIEPFNLASSLSLIIAQRLARRLCDHCKATDDSPDIFLRHSIPNSQI
IYKASSQGCNECNQGYSGRVGIYEVMPFSDQLKNSLIEKPNALAIENLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=92314 OCU90_RS14455 WP_061022518.1 3276894..3278579(+) (pilB) [Vibrio splendidus strain LMG 19031]
GTGCTCACCAACCTCCCAACAATTTTTCGTCAAGCTAACCTTCTTAGTCTGACTCAAGAACAAGCCGTTGCGGAACATGT
GCAAGCTTCAGGTGTATCTACGCCTGAAGCTTTGCTCGTTCTTGATTTCTTCAGCGGCGATTCCTTAGCAAGTAATATCA
AAACCATTTTCGGTTTACCGTTAGTACAACTAGCCAGTACTGACTACGAAACCTTGTGTGAGCAATTGGGGCTTCGTGAA
TTGATCACCAAATATCGAGCAATCCCGATTTCAGTTTCTAGTTCTACTCTTACGCTCGCCTCGGCTGATCCGACCGACTT
ACAAGCTGAAGACGACTTTCGCTTTGCGACAGGATTACAGATTGAATTGGTTGTCGCTAATTACTCTGAACTAGAAGGTG
CAATACGAAAGCTGTATGGCCGTTCTATTTCCGGGCAAGACTCCAAGCGTAAAGAGATCACTCAAGATGAGCTCGCTAAC
CTTGTTGAGATATCTGATGACGAGATAACGTCGATAGAAGACCTCAGCCAAGATGACTCCCCTGTCAGTCGCTTTATCAA
TCAGATTCTAGTCGATGCAGCACGTAAAGGCGCATCTGATATCCACTTCGAACCTTATGAAGAAAACTACCGCGTACGTC
TACGTTGCGATGGCATCCTTGTCGAAATTCAACAGCCCGCTTCTCACTTAAGTCGTCGTCTATCGGCGCGCTTGAAGATT
CTCGCCAAACTTGATATCGCCGAACGTCGTTTGCCTCAAGATGGTCGTATCAAACTGCGCTTAAATGACGAACTGGCGAT
TGATATGCGTGTATCGACGCTGCCAACATTATGGGGAGAAAAGATCGTTCTTCGTCTTCTAGACAGCAGCGCTGCCAATC
TCGATATCGATAAGCTCGGTTACAGCGAGGATCAAAAAGCACTCTACCTCAATGCCCTAAAACGCCCGCAAGGAATGATC
TTAATGACCGGGCCAACGGGCAGCGGTAAAACGGTTTCTCTTTATACTGGCCTTCGAGTGCTCAACACCACTGAACGCAA
CATCTCAACAGCCGAAGACCCTGTTGAAATCAACCTGTGTGGTATCAATCAGGTACAAGTGTCACCTAAGATCGGCTTTG
GTTTCGCTGAAGCATTGCGATCATTTTTACGACAAGATCCTGATGTGGTGATGGTCGGAGAGATCCGTGATTTGGAAACC
GCAGAGATCGCGATCAAAGCCTCGCAAACAGGCCACTTAGTGCTTTCGACACTGCACACCAATTCGGCAGCAGAAACGGT
GACTCGCCTCTCTCATATGGGAATAGAACCTTTTAACCTCGCTTCATCATTAAGCCTGATTATCGCCCAACGACTGGCAA
GACGATTGTGCGACCACTGTAAAGCCACTGATGACTCGCCTGATATATTTCTACGCCACTCTATTCCCAATAGCCAAATC
ATCTACAAAGCCAGCTCACAAGGATGCAATGAGTGTAATCAGGGATACTCGGGGCGAGTCGGTATCTATGAAGTCATGCC
TTTTAGCGACCAATTGAAAAATAGCCTGATCGAAAAACCGAACGCGTTAGCCATTGAAAACTTAGCGCGCCGAGAAGGCA
TGAGAACTTTACAAGAATCAGGGCTAGATAAACTGCTTGAAGGCACCACCAGCTATCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

72.954

100

0.731
  pilB Vibrio campbellii strain DS40M4

70.107

100

0.702
  pilB Vibrio parahaemolyticus RIMD 2210633

69.929

100

0.701
  pilB Acinetobacter baumannii D1279779

50.575

93.048

0.471
  pilB Legionella pneumophila strain ERS1305867

49.237

93.405

0.46
  pilB Acinetobacter baylyi ADP1

49.326

92.513

0.456
  pilF Neisseria gonorrhoeae MS11

43.964

98.93

0.435

Multiple sequence alignment