Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OCV49_RS02580 Genome accession   NZ_AP025499
Coordinates   508589..510274 (-) Length   561 a.a.
NCBI ID   WP_192852302.1    Uniprot ID   -
Organism   Vibrio lentus strain LMG 21034     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 503589..515274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV49_RS02550 rplS 503926..504279 (+) 354 WP_009847607.1 50S ribosomal protein L19 -
  OCV49_RS02555 yacG 504744..504941 (-) 198 WP_017056605.1 DNA gyrase inhibitor YacG -
  OCV49_RS02560 zapD 505023..505763 (-) 741 WP_017108712.1 cell division protein ZapD -
  OCV49_RS02565 coaE 505790..506404 (-) 615 WP_192852304.1 dephospho-CoA kinase -
  OCV49_RS02570 pilD 506407..507276 (-) 870 WP_192852303.1 A24 family peptidase Machinery gene
  OCV49_RS02575 pilC 507345..508574 (-) 1230 WP_102267283.1 type II secretion system F family protein Machinery gene
  OCV49_RS02580 pilB 508589..510274 (-) 1686 WP_192852302.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCV49_RS02585 pilA 510275..510685 (-) 411 WP_192852301.1 pilin Machinery gene
  OCV49_RS02590 nadC 510940..511830 (-) 891 WP_017079961.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OCV49_RS02595 ampD 511914..512513 (+) 600 WP_192852300.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OCV49_RS02600 pdhR 512997..513758 (+) 762 WP_009847596.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62018.62 Da        Isoelectric Point: 4.9253

>NTDB_id=92185 OCV49_RS02580 WP_192852302.1 508589..510274(-) (pilB) [Vibrio lentus strain LMG 21034]
MLTNLPTVLRQADLLSLTQEQAVAEHVHASGVSTPEALLVLDIFTGESLANNIHAIFGLPLVQLGNTDYEALCEQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVEVSDDEMTSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLCGINQVQVAPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKAADDSPDIFLRHSIPNNQT
IYKANPQGCNECNQGYSGRVGIYEVMPFSDQLKSSLIDKPNALAIENLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=92185 OCV49_RS02580 WP_192852302.1 508589..510274(-) (pilB) [Vibrio lentus strain LMG 21034]
GTGCTAACCAACCTCCCAACCGTTCTCCGTCAGGCTGACTTACTCAGCCTGACTCAAGAACAAGCCGTTGCGGAACATGT
ACATGCTTCAGGCGTTTCTACGCCTGAAGCTTTACTTGTTCTAGACATCTTCACCGGCGAGAGTCTCGCAAATAACATTC
ACGCCATCTTCGGCTTGCCGTTGGTACAACTTGGCAATACGGACTATGAAGCTTTATGCGAACAGTTAGGGCTTCGTGAG
CTGATCACCAAGTATCGCGCCATTCCAATTTCAGTTTCTAGCTCAACTCTTACACTCGCTTCAGCCGATCCAACCGACTT
ACAAGCTGAAGATGATTTCCGCTTTGCGACGGGATTACAAATCGAATTAGTTGTCGCTAACTACTCTGAACTAGAAGGCG
CAATACGAAAGCTGTATGGACGCTCTATTTCTGGGCAAGACTCCAAGCGTAAAGAGATCACCCAAGATGAACTCGCCAAC
CTAGTTGAAGTCTCTGACGATGAGATGACTTCAATTGAAGACCTCAGCCAAGACGACTCCCCTGTCAGCCGTTTTATCAA
TCAAATACTGGTTGATGCGGTGCGTAAAGGCGCTTCCGATATCCACTTCGAACCTTATGAAGAAAACTATCGTGTGCGCT
TACGTTGCGATGGCATCCTTGTTGAAATACAACAGCCCGCTTCTCATTTAAGTCGCCGTTTATCTGCTCGTTTAAAGATC
CTCGCTAAACTGGATATTGCCGAACGTCGCTTGCCTCAAGATGGTCGTATCAAGTTGCGGCTAAACGATGAACTGGCGAT
TGATATGCGTGTATCTACGCTGCCAACCTTGTGGGGTGAGAAGATCGTTCTTCGTCTTCTAGATAGCAGCGCTGCTAATC
TAGATATCGATAAGTTGGGTTACAGCGAGGATCAAAAAGCACTCTATCTCAATGCATTGAAGCGCCCACAAGGAATGATC
TTAATGACCGGCCCAACCGGCAGTGGTAAAACCGTTTCTCTCTACACTGGCCTTCGGGTACTTAACACCACAGAGCGCAA
CATCTCAACAGCTGAAGATCCGGTCGAGATCAATCTATGTGGTATCAATCAAGTTCAAGTCGCACCCAAAATCGGTTTTG
GGTTTGCTGAAGCATTGCGATCATTTTTACGACAAGATCCCGATGTGGTGATGGTGGGGGAGATCCGCGATTTAGAAACC
GCAGAGATCGCGATCAAAGCATCGCAAACCGGTCACCTAGTCCTTTCGACATTGCACACCAACTCCGCCGCAGAAACCGT
GACTCGATTGGCTCACATGGGAATTGAACCCTTTAATCTTGCTTCATCATTAAGCCTGATTATCGCCCAACGACTGGCAA
GACGATTGTGCAATCACTGTAAAGCCGCTGACGACTCTCCGGACATATTTCTGCGTCACTCTATCCCTAACAACCAAACG
ATCTACAAAGCCAATCCTCAGGGGTGTAATGAGTGTAATCAGGGATACTCTGGGCGAGTGGGCATCTATGAAGTTATGCC
GTTTAGCGACCAACTGAAAAGCAGCCTAATCGACAAACCGAACGCGTTAGCCATTGAAAACTTGGCGCGCCGAGAAGGCA
TGCGAACACTGCAAGAATCAGGGCTAGATAAATTGCTGGAAGGCACCACCAGCTATCAAGAACTACAACGTGTTCTGTAC
CTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.89

100

0.742
  pilB Vibrio campbellii strain DS40M4

70.285

100

0.704
  pilB Vibrio parahaemolyticus RIMD 2210633

70.285

100

0.704
  pilB Acinetobacter baumannii D1279779

51.252

92.513

0.474
  pilB Legionella pneumophila strain ERS1305867

49.618

93.405

0.463
  pilB Acinetobacter baylyi ADP1

49.522

93.226

0.462
  pilF Neisseria gonorrhoeae MS11

43.784

98.93

0.433

Multiple sequence alignment