Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VQ575_RS02200 Genome accession   NZ_CP142104
Coordinates   481364..483436 (+) Length   690 a.a.
NCBI ID   WP_039592429.1    Uniprot ID   -
Organism   Pseudomonas frederiksbergensis strain YE17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 476364..488436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VQ575_RS02180 (VQ575_02180) comM 478576..479640 (+) 1065 WP_039592425.1 pilus assembly protein PilM Machinery gene
  VQ575_RS02185 (VQ575_02185) - 479640..480206 (+) 567 WP_039592426.1 PilN domain-containing protein -
  VQ575_RS02190 (VQ575_02190) pilO 480203..480826 (+) 624 WP_039592427.1 type 4a pilus biogenesis protein PilO -
  VQ575_RS02195 (VQ575_02195) - 480823..481350 (+) 528 WP_039592428.1 pilus assembly protein PilP -
  VQ575_RS02200 (VQ575_02200) pilQ 481364..483436 (+) 2073 WP_039592429.1 type IV pilus secretin PilQ Machinery gene
  VQ575_RS02205 (VQ575_02205) aroK 483441..483959 (+) 519 WP_039592430.1 shikimate kinase AroK -
  VQ575_RS02210 (VQ575_02210) aroB 484107..485207 (+) 1101 WP_039592431.1 3-dehydroquinate synthase -
  VQ575_RS02215 (VQ575_02215) - 485218..486804 (+) 1587 WP_039592432.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74596.56 Da        Isoelectric Point: 5.1152

>NTDB_id=921618 VQ575_RS02200 WP_039592429.1 481364..483436(+) (pilQ) [Pseudomonas frederiksbergensis strain YE17]
MNRIFSALGISLWTALLSPMVLGASLKTLDVAALPGDRVELKLSFDGPPPTPRGYTTEQPARIALDLPGVTNQLARKSHD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDTRIEGNNLFVIVGKGATARSAALAPQPAGSAPSFTRSSAPAGKAIRGVD
FQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILDFPRTQLPEPLRVRLDVKDFATPVQFVNGSAGSDRATISIEPSGTFD
YSTYQTDNKLTVSVRPMTVEDLQKRNSDRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPW
DQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKAD
ERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNSSGVTTGD
STTIGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
ASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=921618 VQ575_RS02200 WP_039592429.1 481364..483436(+) (pilQ) [Pseudomonas frederiksbergensis strain YE17]
ATGAACAGGATTTTTTCAGCCCTCGGTATTTCGCTATGGACAGCGCTGTTGTCGCCGATGGTTCTTGGGGCCAGCCTGAA
GACACTCGACGTTGCGGCGTTGCCGGGGGACCGCGTGGAACTGAAGCTCAGCTTCGATGGGCCGCCACCCACGCCCCGGG
GCTATACCACTGAACAGCCGGCCCGAATCGCGCTGGATCTGCCCGGGGTAACCAACCAACTGGCCCGCAAGAGTCACGAT
CTTGGAGGAGGCAATGCACGCAGCGCCACCGTGGTCGAGGCGGGCGATCGTACCCGGCTGATCATTGGCCTGACCCAATT
GGCGCCCTATGACACCCGGATCGAAGGCAACAACCTCTTCGTGATAGTGGGCAAGGGCGCGACCGCCAGGTCCGCCGCGC
TGGCACCGCAGCCGGCGGGATCCGCCCCGTCCTTCACCAGGTCCAGTGCGCCGGCAGGCAAGGCGATCCGTGGGGTGGAC
TTCCAGCGCGGGACACAAGGCGAAGGCAACGTGGTCATCGACTTGTCCGATCCGTCCATTGCGCCGGACATTCAGGAACG
CGAAGGCAAGATCATCCTTGATTTCCCCAGGACCCAGTTGCCCGAACCCCTGCGGGTACGCCTGGACGTCAAGGATTTCG
CCACCCCGGTGCAATTCGTCAATGGCAGCGCCGGCTCCGACCGCGCAACCATCAGCATCGAACCCAGCGGCACCTTCGAC
TATTCCACCTACCAGACCGACAACAAGTTGACCGTGAGCGTCCGGCCGATGACGGTCGAGGACTTGCAGAAACGCAACAG
CGATCGCGCCGCCTACAGCGGCGAGAAGTTGTCGCTCAACTTCCAGGACATCGAAGTGCGTTCGGTGCTGCAACTCATCG
CCGATTTCACCAACCTGAACCTGGTGGCCAGCGACACCGTGCAGGGTGGCATCACCCTGCGGTTGCAGAACGTGCCGTGG
GACCAGGCACTGGATCTGGTGCTCAAGACCAAGGGGCTCGACAAGCGCAAGGTCGGCAATGTCCTGCTGGTAGCACCCGC
CGACGAGATTGCGGCACGCGAACGCCAGGAGCTCGAATCCCAGAAGCAGATTGCCGACCTGGCACCGCTGAGGCGCGAGC
TGTTGCAGGTCAACTATGCCAAGGCCGCCGACATCGCCAAGCTGTTCCAGTCGGTCACCAGTGCCGAGGCGAAGGCGGAC
GAACGGGGCACGATCACGGTTGATGAGCGAACCAACAATATCATCGCCTACCAGACCCAGGACCGGCTCGACGAGCTGCG
CCGAATCGTTGCGCAATTGGACATCCCGGTGCGCCAAGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGATTATG
ACAAGAGCCTGGGCGTGCGCTGGGGTGGCTCGATACAGAACAAGGGCAACTGGAATTCTTCCGGTGTGACCACTGGAGAC
TCCACTACGATTGGTACGCCAGGCAGCACCAGCACCAACCAACCGTTTGTCGACCTCGGTGCATCAGGCAATACGTCCGG
GATCGGCATCGCCTTCATCACCGACAACGTGCTGCTGGACCTTGAACTGACGGCCATGGAAAAAACCGGCAATGGCGAGA
TCGTTTCCCAGCCCAAGGTGGTGACCTCCGACAAGGAGACCGCGAAAATCCTCAAGGGCACGGAGATTCCCTACCAGGAA
GCCAGCTCCAGCGGCGCCACCTCGGTGTCGTTCAAGGAAGCCTCGTTGTCGCTGGAGGTTACGCCGCAGATCACCCCGGA
CAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGATTACCTGAACAAGGTCCAGGATGTACCGCCGATCA
AGAAAAACGAGGTCAACGCCAAGGTGCTGGTCAATGACGGCGAGACCATTGTGATTGGTGGCGTTTTCTCAAATACTCAG
AGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTAGTCTCGGA
GAAAAAATCCGAGCTGCTGGTGTTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-121)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Bacterial type II/III secretion system short domain (Secretin_N)

(374-439)

Secretin and TonB N terminus short domain (STN)

(300-346)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.147

100

0.758
  comQ Acinetobacter baylyi ADP1

42.113

100

0.422
  pilQ Acinetobacter baumannii D1279779

38.514

100

0.413
  pilQ Acinetobacter baumannii strain A118

38.514

100

0.413
  pilQ Legionella pneumophila strain Lp02

38.667

97.826

0.378
  pilQ Legionella pneumophila strain ERS1305867

38.667

97.826

0.378