Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M3O59_RS15470 Genome accession   NZ_CP142084
Coordinates   3561721..3563451 (-) Length   576 a.a.
NCBI ID   WP_116906311.1    Uniprot ID   A0A3E1KH64
Organism   Xanthomonas nasturtii strain 10053     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3556721..3568451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3O59_RS15455 (M3O59_015430) sucC 3557002..3558171 (-) 1170 WP_064634549.1 ADP-forming succinate--CoA ligase subunit beta -
  M3O59_RS15460 (M3O59_015435) - 3558404..3560017 (+) 1614 WP_064634548.1 PAS domain-containing sensor histidine kinase -
  M3O59_RS15465 (M3O59_015440) pilR 3560197..3561591 (+) 1395 WP_147308524.1 sigma-54 dependent transcriptional regulator Regulator
  M3O59_RS15470 (M3O59_015445) pilB 3561721..3563451 (-) 1731 WP_116906311.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M3O59_RS15475 (M3O59_015450) - 3563497..3563937 (-) 441 WP_116906312.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  M3O59_RS15480 (M3O59_015455) - 3564164..3564622 (-) 459 WP_116906313.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  M3O59_RS15485 (M3O59_015460) pilC 3564990..3566243 (+) 1254 WP_116906314.1 type II secretion system F family protein Machinery gene
  M3O59_RS15490 (M3O59_015465) - 3566250..3567113 (+) 864 WP_046343941.1 A24 family peptidase -
  M3O59_RS15495 (M3O59_015470) coaE 3567127..3567732 (+) 606 WP_116906315.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62675.90 Da        Isoelectric Point: 5.6021

>NTDB_id=921396 M3O59_RS15470 WP_116906311.1 3561721..3563451(-) (pilB) [Xanthomonas nasturtii strain 10053]
MSALTLNLVGITGIARRLVQDGALDESAARTAMDQAALAKVPLPQWFAEKKLVTASQLAAANAVEFGMPLMDVSVFDASQ
NAIKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLGN
DDDDIGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPV
KLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPE
HALLAEGFTPEQIANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQRIGIRDLRQSALVKA
AHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=921396 M3O59_RS15470 WP_116906311.1 3561721..3563451(-) (pilB) [Xanthomonas nasturtii strain 10053]
ATGAGCGCGCTAACCTTGAACTTAGTTGGGATCACAGGTATTGCAAGACGCCTCGTTCAAGATGGGGCCTTAGATGAGTC
AGCAGCGCGTACGGCGATGGACCAAGCCGCTTTGGCAAAGGTTCCTTTGCCACAATGGTTCGCCGAAAAAAAGCTGGTTA
CAGCGTCTCAGCTAGCTGCTGCCAACGCCGTAGAATTCGGTATGCCTTTGATGGACGTGTCGGTCTTCGACGCCAGCCAG
AACGCAATCAAGCTGGTCAGCGAAGAGTTGCTCCAGAAGTACCAGGTGCTGCCGTTGTTCAAGCGCGGTAACCGGTTGTT
CGTGGGGGTGAGCAACCCGACCCAGACCCGTGCGCTGGACGACATCAAGTTCCATACCAATTTGGTGGTCGAGCCGATTC
TGGTCGACGAGGACCAGATCCGTCGCACCCTGGAACAGTGGCAGGCCAGCAATGCCGCGCTTGGGTCGTCGCTCGGCAAT
GACGATGACGATATAGGTGACCTGGACGTGTCGGCCGGCGACGAGGACATGGGCGCCGGTGGGGATTCCGGGGTCGATGC
CAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACATCC
ATTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGCATCGATGGGTTGCTGAAGAACGTGGCCAAGGCGCCGGTG
AAGCTGAGCCAACGCATCGCGGCGCGATTGAAGGTGATGTCGCAGCTGGATATCGCCGAAAAGCGGGTGCCGCAGGACGG
GCGCATCAAGCTCAATCTGTCCAAGACCAAGCAAATCGACTTCCGTGTGAGTACCTTGCCGACGCTGTTTGGCGAGAAGG
TGGTGCTGCGTATCCTCGATGCCAGTGCGGCCAAGCTGGGGATCGAGAAGCTGGGTTATGAGGCGGATCAGCAGAAGTTG
TTCCTGGATGCGATCCATAAGCCCTACGGGATGGTGCTGGTGACCGGTCCGACCGGTTCGGGCAAGACGGTGTCGTTGTA
TACGGCGCTAGGCATCCTCAACGATGAGACGCGCAACATTTCCACGGCCGAGGATCCGGTCGAAATCCGCTTGCCGGGCG
TCAATCAGGTGCAGCAGAACGTCAAGCGCGGGATGACGTTCGCCGCAGCATTGCGCTCGTTCCTGCGTCAGGATCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAGACTGCCGAGATCGCGATCAAGGCGGCGCAGACCGGTCACATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCACAAACCATCGCGCGTTTGATGAACATGGGGATTGCGCCGTACAACATCACCT
CTTCGGTGACGTTGGTGATTGCGCAGCGCCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACCCTGCCGGAG
CATGCGCTGTTGGCGGAAGGCTTTACGCCCGAGCAAATCGCAAACATCGAGCTGTATGAGGCGGTCGGCTGCGATGAATG
CACCGAAGGTTACAAGGGACGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGGAAG
GCGGTAACGCGATGCAGATTGCCGAGGCGGCGCAGAGGATCGGTATCCGCGACCTGCGCCAGTCGGCCTTGGTCAAGGCG
GCGCATGGGGTGACGAGCCTGGCTGAGATCAATCGGGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1KH64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.536

100

0.557
  pilB Acinetobacter baylyi ADP1

55.19

100

0.554
  pilB Legionella pneumophila strain ERS1305867

52.113

98.611

0.514
  pilB Vibrio cholerae strain A1552

48.691

99.479

0.484
  pilF Neisseria gonorrhoeae MS11

48.063

98.611

0.474
  pilB Vibrio campbellii strain DS40M4

46.221

98.785

0.457
  pilB Vibrio parahaemolyticus RIMD 2210633

46.691

97.049

0.453
  pilB/pilB1 Synechocystis sp. PCC 6803

35.39

100

0.378