Detailed information NTG0921096

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: U0O11_RS00880
• Genome accession: NZ_CP142020
• Coordinates: 191141..192295 (+)
• Length: 384 a.a.
• NCBI ID: WP_043333447.1
• Uniprot ID: A0AAP4TZ60
• Organism: Cobetia sp. D5 isolate Qingdao
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 186141..197295

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U0O11_RS00860 (U0O11_00860)	-	187360..187941 (-)	582	WP_043333437.1	YggT family protein	-
U0O11_RS00865 (U0O11_00865)	proC	188091..188933 (-)	843	WP_324691111.1	pyrroline-5-carboxylate reductase	-
U0O11_RS00870 (U0O11_00870)	-	189022..189774 (-)	753	WP_279836957.1	YggS family pyridoxal phosphate-dependent enzyme	-
U0O11_RS00875 (U0O11_00875)	pilT	189970..191001 (+)	1032	WP_043333445.1	type IV pilus twitching motility protein PilT	Machinery gene
U0O11_RS00880 (U0O11_00880)	pilU	191141..192295 (+)	1155	WP_043333447.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
U0O11_RS00885 (U0O11_00885)	-	192386..193534 (-)	1149	WP_324691114.1	anhydro-N-acetylmuramic acid kinase	-
U0O11_RS00890 (U0O11_00890)	tyrS	193690..194898 (+)	1209	WP_279836955.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 43054.35 Da
• Isoelectric Point: 6.3429

>NTDB_id=921096 U0O11_RS00880 WP_043333447.1 191141..192295(+) (pilU) [Cobetia sp. D5 isolate Qingdao]
MTPHEWLHELLQLMVSKGSSDLFISTGTPPQMKVNGRMVALGDKKLSVDQVRELVLAPMSDMQRERFEEEREANFAHSLP
GVGRFRISAFYQRSQMGMVIRRIQLSIPSLEELRLPEIIKGLSETKRGLVIFVGGTGAGKSTSLAAMIQHRNQTSSGHII
CIEDPIEYIHPHQRSIVTQREVGIDTESFEVALRNTLRQAPDVIMIGEIRSRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPEERHEQVWMDLSLNLKGIVAQQLLPHKSGNTTQRVPAIEVMLRSPLIVDLIRKGAVVEIKDVMKRSQQQGM
MTFDQSLYALHQQGLITEEVALAHADSANDLRLMIKFGDSDSAQEAQLDVLNAASRFSLQGDDD

Nucleotide

• Length: 1155 bp

>NTDB_id=921096 U0O11_RS00880 WP_043333447.1 191141..192295(+) (pilU) [Cobetia sp. D5 isolate Qingdao]
ATGACTCCTCATGAATGGCTGCATGAACTTCTGCAGTTGATGGTCAGCAAGGGCAGCTCGGACCTGTTCATCTCCACCGG
CACACCGCCGCAGATGAAGGTCAATGGCCGCATGGTGGCGCTGGGTGACAAGAAGCTGAGTGTCGATCAGGTCAGAGAGC
TGGTGCTCGCACCGATGAGTGACATGCAGCGCGAGCGCTTCGAGGAAGAGCGCGAGGCCAACTTCGCGCATAGCTTGCCG
GGGGTCGGGCGCTTCAGGATCAGCGCGTTCTACCAGCGCAGCCAGATGGGCATGGTGATTCGCCGCATCCAGCTGTCGAT
TCCCAGCCTGGAAGAGTTGCGGCTGCCGGAGATCATCAAGGGGCTTTCCGAGACCAAGCGTGGTCTGGTGATCTTCGTCG
GCGGCACCGGCGCCGGCAAGTCGACCTCGCTGGCGGCGATGATCCAGCACCGCAACCAGACCTCGAGCGGGCACATCATC
TGCATCGAAGACCCGATCGAGTATATCCACCCTCACCAGCGCTCCATCGTGACGCAGCGTGAAGTGGGGATCGATACCGA
GTCCTTCGAGGTGGCGCTGCGCAATACGCTGCGTCAGGCGCCGGACGTGATCATGATCGGCGAGATCCGCTCGCGTGAGA
CCATGGAGCACGCGCTGACATTCGCCGAGACCGGTCACCTGTGTCTTGCGACGTTGCATGCCAACAATGCCAACCAGGCG
CTGGACCGCATCATCCACTTCTTCCCGGAGGAGCGTCATGAACAGGTGTGGATGGATCTGTCGCTGAACCTCAAGGGCAT
CGTGGCCCAGCAGCTGTTGCCTCACAAGAGTGGCAACACCACCCAGCGCGTGCCGGCCATAGAGGTGATGCTGCGTTCGC
CGCTGATCGTCGACCTGATCCGCAAGGGCGCGGTGGTCGAGATCAAGGACGTCATGAAGCGCTCGCAGCAGCAGGGCATG
ATGACCTTCGATCAATCGCTGTACGCCTTGCATCAGCAGGGCTTGATCACCGAGGAAGTGGCATTGGCGCATGCCGATTC
CGCCAATGACCTGCGCCTGATGATCAAGTTCGGTGATTCCGACAGCGCCCAGGAAGCCCAGCTGGATGTCCTGAATGCCG
CCAGTCGCTTCTCGCTGCAAGGGGATGACGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (84-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	65.625	91.667	0.602
pilU	Vibrio cholerae strain A1552	56.64	96.094	0.544
pilU	Acinetobacter baylyi ADP1	55.932	92.188	0.516
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.348	89.844	0.398
pilT	Neisseria meningitidis 8013	40.816	89.323	0.365
pilT	Pseudomonas aeruginosa PAK	41.742	86.719	0.362