Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OCV19_RS02675 Genome accession   NZ_AP025463
Coordinates   531357..533042 (-) Length   561 a.a.
NCBI ID   WP_065676623.1    Uniprot ID   A0A1C3JFB3
Organism   Vibrio celticus strain CECT 7224     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 526357..538042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV19_RS02645 rplS 526862..527215 (+) 354 WP_004735514.1 50S ribosomal protein L19 -
  OCV19_RS02650 yacG 527514..527711 (-) 198 WP_010435916.1 DNA gyrase inhibitor YacG -
  OCV19_RS02655 zapD 527793..528533 (-) 741 WP_017063237.1 cell division protein ZapD -
  OCV19_RS02660 coaE 528560..529174 (-) 615 WP_065676620.1 dephospho-CoA kinase -
  OCV19_RS02665 pilD 529177..530046 (-) 870 WP_065676621.1 A24 family peptidase Machinery gene
  OCV19_RS02670 pilC 530112..531341 (-) 1230 WP_065676622.1 type II secretion system F family protein Machinery gene
  OCV19_RS02675 pilB 531357..533042 (-) 1686 WP_065676623.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCV19_RS02680 - 533042..533485 (-) 444 WP_065676624.1 type IV pilin protein -
  OCV19_RS02685 nadC 533740..534630 (-) 891 WP_017065901.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OCV19_RS02690 ampD 534714..535313 (+) 600 WP_065676625.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OCV19_RS02695 pdhR 535797..536558 (+) 762 WP_009847596.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62172.94 Da        Isoelectric Point: 4.9277

>NTDB_id=91695 OCV19_RS02675 WP_065676623.1 531357..533042(-) (pilB) [Vibrio celticus strain CECT 7224]
MLTNLPTVLRQADLLSLTQEQAVAEHIQASGVSTPEALLVLDIFTGESLAHKIQAIFGLPLVQLANTDYEILCDQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVEVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDDLAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKVLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKEVDDSPDIFLRHSIPNSQT
IYKANTQGCNECNQGYSGRVGIYEVMPLTDKLKTSLISQPNALAIEDLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=91695 OCV19_RS02675 WP_065676623.1 531357..533042(-) (pilB) [Vibrio celticus strain CECT 7224]
ATGCTCACCAACCTCCCAACCGTTCTTCGTCAGGCTGACTTACTTAGCCTGACTCAAGAACAAGCCGTTGCGGAACATAT
TCAAGCTTCAGGTGTTTCTACGCCTGAAGCTTTGCTCGTTCTAGACATCTTCACAGGCGAGAGTCTCGCACATAAGATTC
AGGCCATCTTCGGTTTGCCGCTAGTACAACTTGCCAACACTGACTATGAAATCTTGTGCGACCAATTAGGGCTTCGCGAG
CTGATCACTAAATATCGCGCCATTCCAATTTCAGTTTCTAGCTCAACTCTTACACTCGCCTCGGCCGATCCAACCGACTT
ACAAGCGGAAGACGACTTTCGATTCGCGACAGGGTTGCAGATCGAATTGGTCGTCGCCAACTACTCAGAACTAGAAGGCG
CAATACGAAAGCTGTATGGCCGTTCGATTTCCGGGCAAGACTCTAAACGTAAAGAGATCACTCAAGATGAACTCGCTAAC
CTAGTTGAGGTTTCTGACGACGAGATAACATCGATTGAAGACCTCAGCCAAGACGATTCCCCTGTCAGCCGCTTTATCAA
TCAGATACTGGTTGATGCGGTGCGTAAAGGCGCATCAGATATCCACTTCGAACCTTATGAAGAGCACTACCGAGTACGAC
TGCGTTGTGATGGCATCCTTGTTGAAATACAACAACCCGCTTCCCATTTAAGTCGCCGTTTATCTGCTCGTCTGAAAATA
CTCGCCAAGCTTGATATTGCCGAACGTCGCTTGCCTCAAGATGGTCGTATTAAGTTGCGCTTGAACGACGACCTCGCGAT
TGATATGCGTGTATCAACATTGCCTACCTTGTGGGGTGAGAAGATTGTACTTCGACTGCTTGATAGCAGCGCTGCTAATT
TAGATATCGATAAGTTAGGTTACAGCGAAGATCAAAAAGTACTCTACCTCAATGCCCTGAAGCGCCCACAAGGGATGATC
TTAATGACAGGGCCAACCGGCAGCGGAAAAACCGTCTCCCTTTATACTGGCCTTCGCGTTCTTAACACTACGGAACGCAA
CATCTCAACAGCTGAAGACCCAGTGGAAATTAACCTGTGTGGTATCAATCAAGTACAAGTAACACCTAAGATCGGCTTTG
GGTTTGCTGAGGCACTGCGATCATTTTTACGACAAGACCCTGATGTTGTGATGGTTGGAGAGATCCGCGACTTGGAAACG
GCAGAGATCGCGATCAAAGCATCGCAAACGGGTCACCTGGTTCTTTCGACGCTGCACACTAACTCCGCCGCAGAAACCGT
CACTCGACTCGCTCATATGGGGATAGAACCCTTTAACCTCGCTTCATCACTAAGTTTGATTATCGCCCAACGACTGGCAA
GACGATTGTGCAACCATTGCAAAGAAGTTGACGACTCGCCAGACATATTTCTGCGTCACTCGATTCCTAACAGCCAAACC
ATCTACAAAGCTAACACTCAAGGTTGTAATGAGTGTAATCAAGGATACTCAGGTCGCGTTGGTATCTACGAAGTTATGCC
TTTGACCGACAAACTCAAGACAAGTCTTATCAGTCAACCCAATGCGCTGGCGATTGAAGATCTCGCGCGCCGAGAAGGCA
TGCGAACATTGCAAGAGTCAGGTCTGGATAAACTGCTTGAAGGCACCACCAGCTACCAAGAACTGCAACGTGTTCTGTAC
CTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C3JFB3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.582

100

0.74
  pilB Vibrio campbellii strain DS40M4

70.107

100

0.702
  pilB Vibrio parahaemolyticus RIMD 2210633

70.107

100

0.702
  pilB Acinetobacter baumannii D1279779

51.06

92.513

0.472
  pilB Legionella pneumophila strain ERS1305867

49.809

93.405

0.465
  pilB Acinetobacter baylyi ADP1

49.326

92.513

0.456
  pilF Neisseria gonorrhoeae MS11

43.604

98.93

0.431
  pilF Thermus thermophilus HB27

38.772

92.87

0.36

Multiple sequence alignment