Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   U4959_RS01790 Genome accession   NZ_CP141284
Coordinates   384727..386451 (-) Length   574 a.a.
NCBI ID   WP_004958615.1    Uniprot ID   -
Organism   Acinetobacter junii strain H1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 379727..391451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U4959_RS01760 (U4959_01760) - 379794..380120 (+) 327 WP_004958605.1 pyrimidine/purine nucleoside phosphorylase -
  U4959_RS01765 (U4959_01765) rlmB 380310..381059 (+) 750 WP_151831121.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  U4959_RS01770 (U4959_01770) - 381091..382002 (+) 912 WP_004958607.1 DMT family transporter -
  U4959_RS01775 (U4959_01775) coaE 381999..382598 (-) 600 WP_004958609.1 dephospho-CoA kinase -
  U4959_RS01780 (U4959_01780) pilD 382600..383460 (-) 861 WP_324272906.1 A24 family peptidase Machinery gene
  U4959_RS01785 (U4959_01785) pilC 383473..384702 (-) 1230 WP_151690733.1 type II secretion system F family protein Machinery gene
  U4959_RS01790 (U4959_01790) pilB 384727..386451 (-) 1725 WP_004958615.1 type IV-A pilus assembly ATPase PilB Machinery gene
  U4959_RS01795 (U4959_01795) tpiA 386797..387588 (+) 792 WP_004958617.1 triose-phosphate isomerase -
  U4959_RS01800 (U4959_01800) secG 387601..387930 (+) 330 WP_004916847.1 preprotein translocase subunit SecG -
  U4959_RS01820 (U4959_01820) rimP 388553..389077 (+) 525 WP_004916844.1 ribosome maturation factor RimP -
  U4959_RS01825 (U4959_01825) nusA 389115..390599 (+) 1485 WP_004916840.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63664.81 Da        Isoelectric Point: 5.0662

>NTDB_id=916876 U4959_RS01790 WP_004958615.1 384727..386451(-) (pilB) [Acinetobacter junii strain H1]
MSGIHTSPKFSGFIRRIVDDGHIAASNMQSAIDAAKKAQQDIVAHLIEHHRLSPLTIAESISLEFGEPLFDLAVFDTALL
PREITDSKLIQKHRVVPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPVIVEHNKLEKLIDQQFGDSGSFDFGENDD
FDLDNVDVATTDTNNTTEDEDTNNQEESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLAT
RLASRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKELFMSA
LDKPQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMV
GEIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSECKQPIEIPKQSLL
EMGFTEQDLANPDFQIFQPVGCDACREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVMQ
GITSLQEVNRVTSE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=916876 U4959_RS01790 WP_004958615.1 384727..386451(-) (pilB) [Acinetobacter junii strain H1]
ATGTCAGGAATACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGCATAGTCGATGATGGTCACATTGCTGCATCAAA
TATGCAGAGTGCGATTGATGCTGCAAAAAAAGCACAACAAGATATTGTTGCTCACTTAATTGAACATCATCGTCTATCAC
CTCTGACAATTGCGGAAAGCATATCTTTAGAGTTTGGTGAGCCACTCTTTGATCTTGCTGTATTTGATACAGCTCTATTA
CCTCGTGAAATAACTGACAGCAAACTGATTCAAAAACATCGTGTTGTTCCACTCATTCAACGTGGTCAGATTTTGTATGT
TGCGACCAGCAACCCGACGAATATTGATGCATTAGACGCAATCAGGTTCAATACCAAATTGAATATCGAACCTGTGATTG
TTGAGCATAATAAACTCGAAAAACTGATTGATCAGCAATTTGGAGATAGCGGCAGTTTCGATTTTGGGGAAAATGACGAC
TTTGATTTAGATAATGTTGATGTTGCAACGACTGATACAAACAACACGACTGAGGATGAGGATACAAATAATCAAGAAGA
ATCTCCTATCGTAAAATATATCAACAAGTTATTGATTGATGCGATTCGCATGGGAGCCTCTGACTTACATTTTGAGCCTT
ATGAAAAAATGTATCGCGTGCGTTATCGTGTGGATGGTGTACTCAGACAAATTGCTAACCCACCATTACAACTCGCAACT
CGTTTAGCCTCGCGCTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAGCGTATGCCTCAAGATGGTCGTATCAAACT
AAAACTCTCTAAAACTAAAGCGATTGATTTTCGTGTTAACTCACTTCCAACTTTATTTGGAGAGAAACTGGTTCTTCGTA
TCTTAGATCCTTCAAGCGCAATGTTAGGTATTGAAGCCTTAGGCTATGAGGATGAACAAAAAGAACTCTTTATGAGTGCA
TTAGACAAGCCTCAGGGTATGCTTCTGATTACAGGTCCAACAGGCTCAGGGAAAACTGTATCGTTGTATACAGGTTTAAA
TATATTAAACACAGAAGATACCAATATTTCGACCGCAGAAGATCCCGTCGAAATTAACTTAGAAGGTATTAACCAAGTTA
ACGTCAACCCTAAAACAGGTTTAACTTTCTCAGCAGCATTAAAAGCTTTTTTACGTCAAGATCCTGATGTCATCATGGTC
GGTGAGATTCGTGATTTGGAAACAGCTGAAATTGCAATTAAAGCAGCTCAAACAGGTCACATGGTGATGTCGACCCTGCA
TACCAATAGCGCTCCTGAAACACTCACACGATTACGCAATATGGGGGTTCCCTCTTTCAATATTGCAACCTCGGTTAATC
TCGTGATTGCTCAACGTTTAGCCCGCCGCCTATGCTCGGAATGTAAACAACCTATCGAGATTCCTAAGCAGAGCCTTTTA
GAAATGGGCTTCACTGAACAAGATTTAGCTAACCCAGACTTTCAAATTTTTCAACCTGTCGGCTGCGATGCTTGTCGTGA
AGGATATAAAGGGCGTGTCGGTATTTATGAAGTAATGAAAGTGACACCTGAGATTTCCAAGATTATTATGGAAGATGGAA
ATGCTTTACAAATTGCAGCAGCATCTGAAAAGGCTGGTTTCAATAATCTACGCCGTTCTGGTTTAAAAAAGGTCATGCAA
GGGATCACCTCTTTGCAAGAAGTTAACCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.597

100

0.819
  pilB Acinetobacter baylyi ADP1

76.87

100

0.77
  pilB Legionella pneumophila strain ERS1305867

52.974

93.728

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

50

97.213

0.486
  pilF Neisseria gonorrhoeae MS11

50.457

95.296

0.481
  pilB Vibrio cholerae strain A1552

48.917

96.516

0.472
  pilB Vibrio campbellii strain DS40M4

51.451

90.07

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

34.74

100

0.373