Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   U3649_RS06845 Genome accession   NZ_CP141262
Coordinates   1635501..1637219 (-) Length   572 a.a.
NCBI ID   WP_407354112.1    Uniprot ID   -
Organism   Luteimonas sp. R10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1637481..1649376 1635501..1637219 flank 262


Gene organization within MGE regions


Location: 1635501..1649376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U3649_RS06845 (U3649_06810) pilB 1635501..1637219 (-) 1719 WP_407354112.1 type IV-A pilus assembly ATPase PilB Machinery gene
  U3649_RS06850 (U3649_06815) - 1637481..1638332 (+) 852 WP_407354113.1 glycosyltransferase family 2 protein -
  U3649_RS06855 (U3649_06820) - 1638397..1640256 (+) 1860 WP_407354114.1 glycosyltransferase -
  U3649_RS06860 (U3649_06825) - 1640199..1641143 (-) 945 WP_407354115.1 glycosyltransferase family 2 protein -
  U3649_RS06865 (U3649_06830) - 1641155..1642036 (-) 882 WP_407354116.1 glycosyltransferase -
  U3649_RS06870 (U3649_06835) - 1642046..1643512 (-) 1467 WP_407354117.1 MOP flippase family protein -
  U3649_RS06875 (U3649_06840) - 1643509..1644186 (-) 678 WP_407354118.1 methyltransferase domain-containing protein -
  U3649_RS06880 (U3649_06845) - 1644187..1644855 (-) 669 WP_407354119.1 methyltransferase domain-containing protein -
  U3649_RS06885 (U3649_06850) - 1644852..1646006 (-) 1155 WP_407354120.1 glycosyltransferase -
  U3649_RS06890 (U3649_06855) - 1646252..1648135 (-) 1884 WP_407354121.1 tetratricopeptide repeat protein -
  U3649_RS06895 (U3649_06860) - 1648229..1648636 (-) 408 WP_407354122.1 pilin -
  U3649_RS06900 (U3649_06865) pilA/pilAI 1648762..1649181 (-) 420 WP_407354123.1 pilin Machinery gene

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62276.93 Da        Isoelectric Point: 6.1006

>NTDB_id=916500 U3649_RS06845 WP_407354112.1 1635501..1637219(-) (pilB) [Luteimonas sp. R10]
MNAVASANLVGITGIARRLVLDGALDDTAAREALDKATAERKPIAGWLREHKLVSAAQLAAANSIEFGMPVFDPATMDAS
QGAVKLVKEELLRKHCVLPLFKRGGKLFVGTADPTNTHALDEVKFHTNLVVEPILVDEDTLKRTLEVWLEVHDSFGDALN
DADGLEGLDVGGDEAAESATDVKGDDTPVVKFVNKVLVEAIKKGASDIHFEPYETDYRVRLRIDGILKPTAKMPVKLNQR
ITARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDAGAAKLGIDKLGYEENQKALFLEAI
QKPYGMVLVTGPTGSGKTVSLYTALNILNDDVRNISAVEDPVEIRMPGVNQVQMNVKRGMTFAAALRSFLRQDPDVIMVG
EIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTISRLMNMGIAPYNITSSVTLVIAQRLARRLCDRCKKPVELPDVALLA
EGFTADEVHAGIKIYEAVGCDECTNGYKGRTGIYQVMPMSDEIQEIVLQGGNAMQIAEVAERAGINDLRKSALLKAKMGV
TSLAEINRVTKD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=916500 U3649_RS06845 WP_407354112.1 1635501..1637219(-) (pilB) [Luteimonas sp. R10]
ATGAACGCCGTTGCGTCCGCCAACCTGGTCGGCATCACCGGCATCGCCCGGCGCCTGGTGCTGGACGGCGCCCTGGACGA
CACCGCCGCCCGCGAGGCGCTGGACAAGGCCACCGCCGAGCGCAAGCCGATCGCCGGCTGGCTGCGCGAGCACAAGCTGG
TGTCGGCGGCGCAGCTGGCCGCCGCGAACTCGATTGAGTTCGGCATGCCGGTGTTCGACCCGGCCACGATGGATGCCAGC
CAGGGCGCGGTGAAGCTGGTCAAGGAAGAGCTGCTGCGCAAGCACTGTGTGCTGCCGCTGTTCAAACGCGGCGGCAAGCT
GTTCGTGGGCACCGCCGACCCCACCAATACCCACGCCCTGGACGAGGTGAAGTTCCACACCAACCTGGTGGTGGAGCCGA
TCCTGGTCGACGAGGATACGCTGAAGCGCACGCTGGAGGTCTGGCTGGAGGTCCACGACAGCTTCGGCGATGCCCTGAAC
GATGCCGACGGCCTGGAAGGGCTGGACGTGGGCGGCGACGAGGCCGCCGAGAGCGCCACGGATGTCAAGGGCGACGACAC
CCCCGTGGTGAAATTCGTCAACAAGGTGCTGGTGGAGGCGATCAAGAAAGGGGCCTCGGACATCCATTTCGAGCCGTACG
AGACCGATTACCGGGTGCGGCTGCGCATCGACGGTATCCTCAAGCCCACGGCGAAGATGCCGGTCAAGCTCAACCAGCGC
ATCACCGCCCGTCTGAAGGTGATGGCGCAGTTGGACATCGCCGAAAAGCGGGTACCGCAGGACGGCCGCATCAAGCTGAA
CCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCCTGCCCACCCTGTTCGGCGAGAAGGTAGTGCTGCGTATCC
TCGACGCCGGCGCCGCCAAGCTAGGGATCGACAAGCTGGGCTATGAGGAGAACCAGAAGGCGCTGTTTCTCGAGGCCATC
CAGAAGCCCTACGGCATGGTGCTGGTCACCGGCCCCACGGGCTCCGGCAAGACGGTGTCGCTGTATACGGCGCTGAACAT
CCTCAACGACGACGTGCGCAACATCTCCGCCGTCGAGGATCCTGTGGAGATCCGCATGCCGGGCGTCAATCAGGTGCAGA
TGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGCCAGGACCCGGATGTGATCATGGTTGGC
GAGATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTCGACGCTGCACAC
CAACGATGCGCCGCAGACCATCTCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCTCGGTGACGCTGG
TGATCGCCCAGCGGCTGGCACGGCGCCTGTGTGACCGCTGCAAGAAGCCAGTGGAACTGCCGGACGTGGCGCTGCTGGCC
GAAGGGTTCACCGCCGACGAGGTGCACGCCGGCATCAAGATCTATGAGGCGGTGGGCTGCGACGAGTGCACCAACGGCTA
CAAGGGTCGCACCGGCATCTACCAGGTGATGCCGATGAGCGACGAGATCCAGGAAATCGTGCTGCAGGGCGGCAACGCGA
TGCAGATCGCCGAGGTGGCGGAGCGGGCCGGGATCAACGACCTGCGGAAATCGGCACTGCTGAAGGCGAAGATGGGGGTG
ACCAGCTTGGCGGAGATCAACCGGGTAACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.885

98.427

0.54
  pilB Acinetobacter baylyi ADP1

54.321

99.126

0.538
  pilB Legionella pneumophila strain ERS1305867

52.289

99.301

0.519
  pilB Vibrio cholerae strain A1552

47.909

100

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

49.336

92.133

0.455
  pilB Vibrio campbellii strain DS40M4

48.231

93.881

0.453
  pilF Neisseria gonorrhoeae MS11

52.024

86.364

0.449
  pilB/pilB1 Synechocystis sp. PCC 6803

36.045

100

0.395
  pilF Thermus thermophilus HB27

40.971

90.035

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.476

94.056

0.362