Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   U5817_RS20330 Genome accession   NZ_CP141259
Coordinates   4332823..4334538 (+) Length   571 a.a.
NCBI ID   WP_407278588.1    Uniprot ID   -
Organism   Aromatoleum evansii strain MAY27     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4327823..4339538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U5817_RS20305 (U5817_20245) - 4328182..4328955 (-) 774 WP_407278584.1 M48 family metallopeptidase -
  U5817_RS20310 (U5817_20250) - 4328952..4329704 (-) 753 WP_407278585.1 competence/damage-inducible protein A -
  U5817_RS20315 (U5817_20255) - 4329708..4330478 (-) 771 WP_407278586.1 EI24 domain-containing protein -
  U5817_RS20320 (U5817_20260) ffh 4330491..4331849 (-) 1359 WP_169125940.1 signal recognition particle protein -
  U5817_RS20325 (U5817_20265) - 4331892..4332719 (+) 828 WP_407278587.1 inner membrane protein YpjD -
  U5817_RS20330 (U5817_20270) pilB 4332823..4334538 (+) 1716 WP_407278588.1 type IV-A pilus assembly ATPase PilB Machinery gene
  U5817_RS20335 (U5817_20275) pilC 4334555..4335790 (+) 1236 WP_407278589.1 type II secretion system F family protein Machinery gene
  U5817_RS20340 (U5817_20280) pilD 4335845..4336696 (+) 852 WP_407278590.1 prepilin peptidase Machinery gene
  U5817_RS20345 (U5817_20285) - 4336821..4337084 (+) 264 WP_169125945.1 FmdB family zinc ribbon protein -
  U5817_RS20350 (U5817_20290) - 4337100..4337726 (+) 627 WP_169125946.1 DUF502 domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62275.43 Da        Isoelectric Point: 5.3366

>NTDB_id=916476 U5817_RS20330 WP_407278588.1 4332823..4334538(+) (pilB) [Aromatoleum evansii strain MAY27]
MAANPQTALSGLARALIQHGRLSEADAAACTKSAANVPNGFILELAQRKLLTTKAVAWFAAETFGYPLLDISSVDRASIP
RDAIDRKLMAKHQVVALGKRQNRLTVATADPSNMRALDEIRFQTGMQVDLIVVEADRLARLAETLSESTEDTLKDLTGEE
FDMDLLQDQEATEQASEDASAEVDDAPVVKFIQKVLIDAINEGASDIHFEPYEKYYRIRVRTDGILREIAQPPLILKEKI
AARIKVISRLDISEKRIPQDGRMKLVLSKNKSIDFRVSSLPTLHGEKIVMRILDASSAMLGVDALGYEPEQKKVLLDAIE
RPYGMILVTGPTGSGKTVSLYTCLNILNKPGVNISTAEDPAEINLAGINQVNVNDKAGLTFAASLRAFLRQDPDVIMVGE
IRDLETAEISIKAAQTGHLVLSTLHTNDAPTTLERLRNMGVAPFNIASSVILITAQRLARRLCTCKQPVDIPVEALLQAG
FSGEDLDGSWRPYGPVGCERCKGSGYKGRLGIYQVMPISEEIAHIIMTNGNSMDIAAQAQREGVRDLRQSGLLKVKQGAT
SLEEVLATTNE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=916476 U5817_RS20330 WP_407278588.1 4332823..4334538(+) (pilB) [Aromatoleum evansii strain MAY27]
ATGGCAGCAAACCCCCAGACCGCCCTGAGCGGACTCGCGCGAGCACTGATCCAGCACGGCCGGCTCTCCGAAGCCGACGC
GGCGGCGTGCACCAAGAGCGCGGCAAACGTCCCGAACGGCTTCATCCTCGAACTTGCACAGCGCAAGCTCCTGACCACCA
AGGCCGTTGCCTGGTTCGCCGCCGAAACCTTCGGCTACCCGCTGCTCGACATTTCGTCAGTCGATCGCGCCAGCATCCCC
CGCGACGCGATCGACCGCAAGCTGATGGCCAAGCACCAGGTCGTCGCCCTGGGGAAGCGCCAGAACCGCCTCACCGTTGC
GACGGCCGATCCGTCCAACATGCGCGCGCTCGACGAGATCCGCTTCCAGACCGGGATGCAGGTCGACCTCATCGTCGTCG
AGGCCGATCGCCTGGCACGCCTTGCCGAAACCCTGTCGGAATCGACCGAGGACACGCTCAAGGACCTGACGGGCGAAGAG
TTCGACATGGACCTCCTGCAGGACCAGGAGGCCACGGAACAGGCCAGCGAGGATGCCTCGGCGGAAGTCGATGACGCCCC
GGTCGTCAAGTTCATTCAGAAGGTCCTGATCGACGCCATCAATGAAGGCGCGTCCGACATCCACTTCGAGCCGTACGAAA
AGTACTACCGCATCCGCGTCCGCACCGACGGCATCCTGCGGGAAATCGCCCAGCCGCCGCTGATCCTGAAGGAGAAGATC
GCGGCACGCATCAAGGTCATCTCGCGCCTGGACATTTCGGAAAAGCGCATCCCGCAGGACGGGCGCATGAAGCTCGTCCT
GTCGAAGAACAAGTCGATCGACTTCCGGGTGTCCTCGCTGCCGACACTGCATGGCGAAAAGATCGTCATGCGTATTCTCG
ATGCATCCTCCGCGATGCTGGGGGTCGACGCGCTCGGTTACGAGCCGGAGCAGAAAAAGGTGCTGCTCGATGCGATCGAA
CGCCCATACGGCATGATCCTCGTGACGGGACCGACCGGATCGGGCAAGACCGTCTCGCTCTACACCTGCCTCAACATCCT
CAACAAGCCCGGCGTCAACATCTCGACGGCCGAGGACCCGGCGGAAATCAACCTGGCCGGCATCAACCAGGTCAACGTGA
ACGACAAGGCCGGCCTGACCTTCGCCGCATCGCTGCGCGCCTTCCTGCGCCAGGATCCGGACGTGATCATGGTCGGCGAA
ATCCGCGACCTCGAGACCGCCGAAATCTCCATCAAGGCGGCGCAGACCGGCCACCTCGTGCTGTCGACGCTGCACACCAA
CGACGCCCCGACCACACTCGAGCGCCTGCGCAACATGGGCGTTGCCCCGTTCAACATCGCCTCCTCCGTCATCCTGATCA
CCGCACAACGCCTGGCGCGACGCCTGTGCACGTGCAAGCAACCGGTCGACATCCCGGTGGAAGCCCTGCTCCAGGCGGGC
TTCAGCGGCGAGGACCTCGACGGCAGCTGGCGCCCATACGGCCCTGTCGGCTGCGAGCGCTGCAAGGGCAGCGGCTACAA
GGGGCGCCTGGGTATCTATCAGGTCATGCCCATCTCCGAGGAAATTGCCCATATCATCATGACCAACGGCAATTCGATGG
ACATTGCGGCACAGGCTCAGCGCGAAGGCGTCCGCGACCTGCGCCAGTCCGGCCTGCTGAAAGTGAAACAGGGTGCGACC
TCGCTCGAGGAAGTGCTCGCCACCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.83

99.124

0.553
  pilB Acinetobacter baumannii D1279779

55.124

99.124

0.546
  pilB Legionella pneumophila strain ERS1305867

52.962

100

0.532
  pilF Neisseria gonorrhoeae MS11

56.439

92.469

0.522
  pilB Vibrio cholerae strain A1552

49.36

95.797

0.473
  pilB Vibrio parahaemolyticus RIMD 2210633

50.958

91.419

0.466
  pilB Vibrio campbellii strain DS40M4

49.513

89.842

0.445
  pilF Thermus thermophilus HB27

40.111

94.746

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.866

92.995

0.38
  pilB/pilB1 Synechocystis sp. PCC 6803

41.851

87.04

0.364