Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   U2T01_RS02270 Genome accession   NZ_CP140438
Coordinates   337322..337783 (+) Length   153 a.a.
NCBI ID   WP_000993718.1    Uniprot ID   A0A9P2XM63
Organism   Acinetobacter baumannii strain 2023CK-01292     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 332322..342783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U2T01_RS02250 (U2T01_02255) - 333713..334600 (+) 888 WP_001017330.1 metal-dependent hydrolase -
  U2T01_RS02255 (U2T01_02260) - 334731..335321 (+) 591 WP_000846931.1 LemA family protein -
  U2T01_RS02260 (U2T01_02265) - 335343..336425 (+) 1083 WP_001246381.1 YgcG family protein -
  U2T01_RS02265 (U2T01_02270) - 336419..336979 (+) 561 WP_000258949.1 TPM domain-containing protein -
  U2T01_RS02270 (U2T01_02275) pilA 337322..337783 (+) 462 WP_000993718.1 pilin Machinery gene
  U2T01_RS02275 (U2T01_02280) - 338025..339653 (+) 1629 WP_001195771.1 PglL family O-oligosaccharyltransferase -
  U2T01_RS02280 (U2T01_02285) bfr 339694..340158 (-) 465 WP_000678123.1 bacterioferritin -
  U2T01_RS02285 (U2T01_02290) - 340403..340597 (-) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  U2T01_RS02290 (U2T01_02295) - 340807..341190 (-) 384 WP_000090000.1 RidA family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15449.52 Da        Isoelectric Point: 4.9218

>NTDB_id=914312 U2T01_RS02270 WP_000993718.1 337322..337783(+) (pilA) [Acinetobacter baumannii strain 2023CK-01292]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQNYVARSQATAGYSEISNMRTGYDTELNDGTAITSLSQVGFTATSSGACS
AIGVTNFGNDGAATNAITCTLKGNPKIAGKIISLSRSATGAWTCSTDIATTDEFLPKGCTGGGTPAVGAITTL

Nucleotide


Download         Length: 462 bp        

>NTDB_id=914312 U2T01_RS02270 WP_000993718.1 337322..337783(+) (pilA) [Acinetobacter baumannii strain 2023CK-01292]
ATGAATGCACAAAAAGGTTTTACATTAATCGAACTTATGATCGTGGTTGCCATTATTGGTATTTTGGCTGCGATTGCGAT
TCCTGCTTATCAAAATTATGTAGCTCGTTCTCAGGCTACAGCTGGATATTCTGAAATTAGTAACATGCGTACGGGTTACG
ACACCGAATTAAATGATGGTACAGCGATTACCTCTCTTTCTCAAGTTGGCTTTACTGCGACTTCATCTGGCGCATGTTCA
GCCATAGGTGTTACAAATTTTGGTAACGATGGCGCAGCTACTAACGCTATTACGTGTACTTTAAAAGGAAATCCAAAAAT
CGCTGGAAAGATTATTTCACTTTCACGTTCAGCGACCGGTGCATGGACTTGTAGTACAGATATTGCCACTACTGACGAAT
TCTTACCAAAAGGATGTACGGGTGGTGGAACACCAGCCGTTGGTGCAATTACTACTTTGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

64.286

100

0.647
  pilA Pseudomonas aeruginosa PAK

43.709

98.693

0.431
  pilA Vibrio cholerae strain A1552

42.581

100

0.431
  pilA Vibrio cholerae C6706

42.581

100

0.431
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.581

100

0.431
  comP Acinetobacter baylyi ADP1

39.744

100

0.405
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.357

93.464

0.405