Detailed information NTG0914201

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: U2S75_RS18240
• Genome accession: NZ_CP140420
• Coordinates: 3817969..3819027 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain 2023CK-00893
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3812969..3824027

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U2S75_RS18215 (U2S75_18210)	aroK	3813244..3813813 (-)	570	WP_000643712.1	shikimate kinase AroK	-
U2S75_RS18220 (U2S75_18215)	pilQ	3813825..3815990 (-)	2166	WP_001017029.1	type IV pilus secretin PilQ family protein	Machinery gene
U2S75_RS18225 (U2S75_18220)	pilP	3816053..3816580 (-)	528	WP_024436689.1	pilus assembly protein PilP	Machinery gene
U2S75_RS18230 (U2S75_18225)	pilO	3816591..3817331 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
U2S75_RS18235 (U2S75_18230)	pilN	3817328..3817969 (-)	642	WP_000201226.1	PilN domain-containing protein	Machinery gene
U2S75_RS18240 (U2S75_18235)	pilM	3817969..3819027 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
U2S75_RS18245 (U2S75_18240)	ponA	3819189..3821744 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
U2S75_RS18250 (U2S75_18245)	-	3821762..3822586 (-)	825	WP_002019666.1	putative RNA methyltransferase	-
U2S75_RS18255 (U2S75_18250)	-	3822589..3822921 (-)	333	WP_000169599.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=914201 U2S75_RS18240 WP_002014066.1 3817969..3819027(-) (pilM) [Acinetobacter baumannii strain 2023CK-00893]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=914201 U2S75_RS18240 WP_002014066.1 3817969..3819027(-) (pilM) [Acinetobacter baumannii strain 2023CK-00893]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTCAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCAGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTATCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423