Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   U2S75_RS18230 Genome accession   NZ_CP140420
Coordinates   3816591..3817331 (-) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain 2023CK-00893     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3811591..3822331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U2S75_RS18210 (U2S75_18205) aroB 3812133..3813215 (-) 1083 WP_001194249.1 3-dehydroquinate synthase -
  U2S75_RS18215 (U2S75_18210) aroK 3813244..3813813 (-) 570 WP_000643712.1 shikimate kinase AroK -
  U2S75_RS18220 (U2S75_18215) pilQ 3813825..3815990 (-) 2166 WP_001017029.1 type IV pilus secretin PilQ family protein Machinery gene
  U2S75_RS18225 (U2S75_18220) pilP 3816053..3816580 (-) 528 WP_024436689.1 pilus assembly protein PilP Machinery gene
  U2S75_RS18230 (U2S75_18225) pilO 3816591..3817331 (-) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  U2S75_RS18235 (U2S75_18230) pilN 3817328..3817969 (-) 642 WP_000201226.1 PilN domain-containing protein Machinery gene
  U2S75_RS18240 (U2S75_18235) pilM 3817969..3819027 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  U2S75_RS18245 (U2S75_18240) ponA 3819189..3821744 (+) 2556 WP_000736677.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=914199 U2S75_RS18230 WP_000076101.1 3816591..3817331(-) (pilO) [Acinetobacter baumannii strain 2023CK-00893]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=914199 U2S75_RS18230 WP_000076101.1 3816591..3817331(-) (pilO) [Acinetobacter baumannii strain 2023CK-00893]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAGCTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTGCCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTAGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACTGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683