Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   U3Q39_RS02955 Genome accession   NZ_CP140298
Coordinates   629332..631410 (+) Length   692 a.a.
NCBI ID   WP_053527827.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain MJL19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 624332..636410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U3Q39_RS02935 (U3Q39_002935) comM 626498..627562 (+) 1065 WP_048328486.1 pilus assembly protein PilM Machinery gene
  U3Q39_RS02940 (U3Q39_002940) pilN 627562..628134 (+) 573 WP_053527824.1 type 4a pilus biogenesis protein PilN -
  U3Q39_RS02945 (U3Q39_002945) pilO 628131..628754 (+) 624 WP_053527825.1 type 4a pilus biogenesis protein PilO -
  U3Q39_RS02950 (U3Q39_002950) pilP 628751..629278 (+) 528 WP_053527826.1 type 4a pilus biogenesis lipoprotein PilP -
  U3Q39_RS02955 (U3Q39_002955) pilQ 629332..631410 (+) 2079 WP_053527827.1 type IV pilus secretin PilQ Machinery gene
  U3Q39_RS02960 (U3Q39_002960) aroK 631417..631962 (+) 546 WP_020307762.1 shikimate kinase AroK -
  U3Q39_RS02965 (U3Q39_002965) aroB 631986..633089 (+) 1104 WP_084902474.1 3-dehydroquinate synthase -
  U3Q39_RS02970 (U3Q39_002970) - 633100..634695 (+) 1596 WP_170799320.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74336.26 Da        Isoelectric Point: 4.8755

>NTDB_id=913585 U3Q39_RS02955 WP_053527827.1 629332..631410(+) (pilQ) [Stutzerimonas stutzeri strain MJL19]
MNTTLSRLGAPVLAAFISPALLAANLNALDVAALPGDRVELRLAFDEPVAMPRGYTLDQPARIALDLPGVSSNLGTKNRE
LGVGNARSVTVVEAQGRTRLVVNLTSLMPYSTRVDGNNLFIVLGEGVSNSTPALASAAATPPVTKPASVKPMGAGKAIEN
IDFRRGEDGAGDVIISLSDPSINPTIEEQGGKIRVVFNKVQLPDSLRARLDVQDFATPVRFVESARLADAANIVIEPTGR
YDYLAYQTDSKLTISVKPLTEDEADKRKADRFVYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVNGNITLRLQNV
PWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTNSSAS
GEDRGSIAVDDRTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVNWRGARVGDNNFVVGGVGG
NRVGAGNNPATALGTFVDLGATGATTGIGIGFITDNTILDLQLSAMEKTGNGEIVSQPKVVTSDKETAKILKGAEVPYQE
ASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDFSQAASTGGVPSIRKNEVNAKVLVADGETIVIGGVFSN
TQSKSVDKVPFLGDLPFVGRVFRRDLVQDRKSELLVFITPRIMNNQAIQASR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=913585 U3Q39_RS02955 WP_053527827.1 629332..631410(+) (pilQ) [Stutzerimonas stutzeri strain MJL19]
ATGAACACTACCCTCTCTCGCTTGGGCGCACCCGTGCTGGCGGCATTTATCTCTCCAGCGCTGCTGGCCGCCAATCTAAA
CGCCTTGGATGTGGCTGCGTTGCCCGGCGACCGTGTTGAGCTTAGGCTGGCGTTTGATGAGCCTGTTGCCATGCCCCGTG
GCTACACCCTAGACCAACCTGCACGCATCGCACTCGACCTTCCTGGTGTTTCCAGTAATCTGGGTACGAAAAATCGAGAG
TTGGGCGTTGGTAACGCACGCAGTGTAACTGTCGTCGAAGCTCAGGGACGTACCCGGTTGGTCGTCAACTTGACCTCGCT
CATGCCGTATTCCACACGCGTGGATGGCAACAATTTGTTTATCGTCCTAGGTGAAGGTGTCAGCAACTCCACGCCGGCGC
TTGCCTCTGCCGCAGCTACCCCTCCAGTTACTAAGCCCGCATCGGTCAAGCCGATGGGGGCGGGTAAAGCCATCGAAAAT
ATCGATTTTCGTCGTGGTGAAGACGGCGCTGGTGATGTAATTATCAGTTTGTCAGATCCATCGATTAATCCGACGATTGA
GGAGCAGGGGGGCAAGATTAGGGTCGTTTTTAATAAGGTGCAGTTGCCAGATTCGCTGCGGGCTCGTCTCGATGTTCAGG
ATTTCGCTACGCCTGTGCGGTTTGTTGAGTCTGCCCGATTAGCGGATGCGGCCAATATTGTTATCGAGCCGACTGGGCGC
TACGATTACCTTGCTTATCAGACGGACAGCAAGCTAACAATTAGCGTTAAGCCGTTGACTGAAGATGAGGCGGATAAGCG
CAAGGCAGATCGGTTTGTTTACTCTGGGGAAAAGCTGTCGCTTAACTTCCAAGATATCGATGTCCGGTCCGTGCTTCAGT
TGATTGCGGATTTTACTGATCTCAATCTGGTTGCGAGTGATACAGTAAATGGAAATATTACTCTTCGTCTGCAGAACGTG
CCTTGGGATCAGGCATTGGATCTGGTCTTGAAAACTAAAGGGCTGGACAAGCGCCAAGTTGGGAACGTTCTACTGGTTGC
GCCTGCGGATGAGATTGCTGCGCGTGAGCGCCAGGAGCTCGAGTCGCAACGCCAAATTGCCGAGCTTGCTCCCTTGCGTC
GTGAAGTGGTACAGGTTAACTATGCCAAGGCGGCGGACATAGCTCGTTTGTTTCAATCAGTTACCAATAGCTCGGCTTCC
GGAGAAGATCGGGGCTCCATAGCTGTGGATGATCGGACGAATAATATCATTGCCTACCAGACCCAAGATCGGCTTGATGA
ACTTCGCCGAATCGTTGCGCAGCTGGATATTCCAGTTCGCCAAGTGATGATCGAGGCTCGTATCGTTGAAGCTAACGTGG
ATTATGATAAGAGCTTAGGTGTTAACTGGCGTGGTGCGCGGGTTGGCGACAACAATTTCGTTGTCGGCGGGGTTGGTGGG
AATCGCGTTGGTGCCGGCAATAATCCGGCTACAGCCTTAGGCACATTCGTTGACCTCGGGGCTACAGGCGCGACTACCGG
CATTGGCATTGGGTTTATTACTGATAACACGATTCTGGATCTTCAGCTTTCGGCAATGGAGAAGACAGGAAACGGGGAGA
TAGTGTCGCAACCGAAGGTTGTAACGTCTGATAAGGAAACCGCAAAAATATTGAAAGGTGCGGAAGTTCCCTATCAGGAG
GCAAGTTCCAGCGGGGCGACCAGTACATCGTTTAAAGAGGCTGCTTTATCTTTGGAGGTTACGCCTCAGATTACACCAGA
TAACCGCATTATCATGGAAGTTAAGGTTACCAAGGACGAACCCGACTTCTCCCAGGCTGCGAGCACAGGCGGAGTTCCGT
CGATCCGTAAGAACGAGGTTAACGCCAAAGTGCTGGTGGCCGATGGAGAAACTATAGTAATCGGTGGTGTCTTTTCTAAT
ACTCAAAGCAAGTCGGTCGATAAGGTTCCTTTCCTTGGTGACCTGCCTTTCGTTGGTCGCGTATTCCGCAGGGATCTCGT
GCAGGACCGGAAATCTGAATTGTTGGTGTTCATTACGCCACGGATCATGAATAATCAGGCGATCCAAGCCAGTCGCTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(525-683)

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-441)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.122

100

0.751
  comQ Acinetobacter baylyi ADP1

42.029

99.711

0.419
  pilQ Acinetobacter baumannii strain A118

39.658

100

0.402
  pilQ Acinetobacter baumannii D1279779

39.658

100

0.402
  pilQ Legionella pneumophila strain Lp02

38.519

97.543

0.376
  pilQ Legionella pneumophila strain ERS1305867

38.37

97.543

0.374