Detailed information NTG0913528

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: U3P88_RS05750
• Genome accession: NZ_CP140297
• Coordinates: 1288994..1289608 (-)
• Length: 204 a.a.
• NCBI ID: WP_061860722.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain UMAF6639
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1283994..1294608

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U3P88_RS05720 (U3P88_05720)	rpsT	1284297..1284563 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
U3P88_RS05725 (U3P88_05725)	holA	1284580..1285621 (-)	1042	Protein_1140	DNA polymerase III subunit delta	-
U3P88_RS05730 (U3P88_05730)	-	1285661..1285812 (-)	152	Protein_1141	hypothetical protein	-
U3P88_RS05735 (U3P88_05735)	-	1285853..1285987 (+)	135	WP_003152870.1	YqzM family protein	-
U3P88_RS05740 (U3P88_05740)	comEC	1286006..1288357 (-)	2352	WP_060674655.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
U3P88_RS05745 (U3P88_05745)	-	1288358..1288927 (-)	570	WP_003152868.1	ComE operon protein 2	-
U3P88_RS05750 (U3P88_05750)	comEA	1288994..1289608 (-)	615	WP_061860722.1	helix-hairpin-helix domain-containing protein	Machinery gene
U3P88_RS05755 (U3P88_05755)	comER	1289667..1290488 (+)	822	WP_061860723.1	late competence protein ComER	-
U3P88_RS05760 (U3P88_05760)	-	1290557..1291294 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
U3P88_RS05765 (U3P88_05765)	rsfS	1291291..1291647 (-)	357	WP_007408260.1	ribosome silencing factor	-
U3P88_RS05770 (U3P88_05770)	yqeK	1291671..1292225 (-)	555	WP_061860724.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
U3P88_RS05775 (U3P88_05775)	-	1292215..1292784 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
U3P88_RS05780 (U3P88_05780)	yhbY	1292795..1293085 (-)	291	WP_061860725.1	ribosome assembly RNA-binding protein YhbY	-
U3P88_RS05785 (U3P88_05785)	aroE	1293079..1293921 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22263.50 Da
• Isoelectric Point: 6.4888

>NTDB_id=913528 U3P88_RS05750 WP_061860722.1 1288994..1289608(-) (comEA) [Bacillus velezensis strain UMAF6639]
MTKAMMTERLRRNLKILVACLAAGAIFICIWLFRSEKEEPVKQQSVLGQTASVSVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNINLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=913528 U3P88_RS05750 WP_061860722.1 1288994..1289608(-) (comEA) [Bacillus velezensis strain UMAF6639]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATCCTGGTCGCTTGTCTTGCGGCAGGGGCGATTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTTGGCCAAACAGCCTCAGTAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATAT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	57.143	96.078	0.549
comEA	Lactococcus lactis subsp. cremoris KW2	35.586	100	0.387
comEA	Latilactobacillus sakei subsp. sakei 23K	33.19	100	0.377
comEA/celA/cilE	Streptococcus mitis NCTC 12261	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae Rx1	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae D39	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae R6	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.493	100	0.377
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363