Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SR908_RS01775 Genome accession   NZ_CP140151
Coordinates   359571..361313 (+) Length   580 a.a.
NCBI ID   WP_246922884.1    Uniprot ID   -
Organism   Chromohalobacter canadensis strain ATCC 43984     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 354571..366313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SR908_RS01755 (SR908_01755) - 355272..356447 (+) 1176 WP_097022952.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  SR908_RS01760 (SR908_01760) - 356452..357933 (-) 1482 WP_322527379.1 TrkH family potassium uptake protein -
  SR908_RS01765 (SR908_01765) - 358197..358484 (+) 288 WP_097022954.1 hypothetical protein -
  SR908_RS01770 (SR908_01770) - 358759..359226 (-) 468 WP_281504880.1 pilin -
  SR908_RS01775 (SR908_01775) pilB 359571..361313 (+) 1743 WP_246922884.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SR908_RS01780 (SR908_01780) pilC 361346..362578 (+) 1233 WP_097022957.1 type II secretion system F family protein Machinery gene
  SR908_RS01785 (SR908_01785) - 362649..363491 (+) 843 WP_378075243.1 prepilin peptidase -
  SR908_RS01790 (SR908_01790) coaE 363502..364140 (+) 639 WP_246922887.1 dephospho-CoA kinase -
  SR908_RS01795 (SR908_01795) yacG 364142..364375 (+) 234 WP_040241746.1 DNA gyrase inhibitor YacG -
  SR908_RS01800 (SR908_01800) - 364448..365392 (-) 945 WP_097023116.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63255.06 Da        Isoelectric Point: 4.9586

>NTDB_id=913054 SR908_RS01775 WP_246922884.1 359571..361313(+) (pilB) [Chromohalobacter canadensis strain ATCC 43984]
MHSSSSSSPPAPGTPLLKGLAARLVAEGLIEESKAQHALEAVHDTHQSVLEYVIEQRWVPAREATQSAAWEYGLPFVDLE
AISTERLPIGEGLSDTLIRRLGVLPLARGERHISVAIPYPSTLAHLDELQFTTGLTAEGVLVATDQLQPAIERYLVQRET
HGMLEELDDASEALENLDLDDVAPLNEGLDTDDDDAPVVRFVHKMMLDAARRGASDIHFEPYESTFRIRFRIDGILVEVA
RPPFNLRARLSARLKVMSRLDISERRLPQDGAIKLRVSASRTIDFRVSTLPTVHGEKIVLRLLDAGATRLGIDALGFSES
QQALFEHALAQPQGMILATGPTGSGKTVTLYTGLNILNTDARNIATAEDPVELKLDGINQVNVLPKIGLDFASALRAFLR
QDPDVVMVGEVRDLETAEISVKAAQTGHLVLSTLHTNSAAETLTRLRNMGVAAYNIASSVTLIVAQRLVRQLCPHCKTSV
ELPRELFFEAGFTAEEIETATCYSATGCTQCTHGYKGRIGLYEVLPVSEAMSKLIMADGNSLELGELARQEGHPDLRRSG
LSKVLAGITSLEEINRVVLE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=913054 SR908_RS01775 WP_246922884.1 359571..361313(+) (pilB) [Chromohalobacter canadensis strain ATCC 43984]
ATGCATTCGTCTTCATCGTCGTCTCCTCCCGCCCCCGGCACGCCGCTTTTGAAAGGGCTCGCCGCACGCCTGGTCGCGGA
AGGCCTGATCGAGGAATCCAAGGCGCAGCATGCCTTGGAGGCCGTGCACGACACGCATCAGTCGGTGCTCGAATACGTCA
TCGAGCAGCGCTGGGTTCCCGCACGAGAAGCCACCCAGAGTGCGGCGTGGGAATATGGCCTGCCGTTCGTCGACCTGGAG
GCCATCAGTACCGAGCGCCTACCCATCGGTGAAGGGCTGTCCGATACCCTGATCCGCCGCCTCGGGGTACTGCCGCTGGC
GCGTGGCGAACGTCACATCAGCGTAGCGATTCCCTATCCCTCGACGCTTGCACACCTCGATGAGCTGCAGTTTACCACCG
GGCTTACCGCCGAAGGCGTGTTGGTAGCCACCGATCAACTGCAGCCAGCCATCGAGCGTTATCTCGTTCAGCGCGAGACG
CATGGCATGCTCGAAGAGCTCGATGATGCCAGCGAGGCACTCGAGAACTTGGATCTCGACGATGTCGCGCCTCTCAATGA
GGGGCTGGACACCGACGATGACGACGCGCCGGTGGTGCGCTTCGTACACAAAATGATGCTCGATGCTGCGCGTCGTGGCG
CTTCGGATATCCATTTCGAGCCCTATGAATCGACCTTCCGCATCCGTTTCCGCATCGATGGCATCCTGGTCGAGGTTGCG
CGCCCGCCCTTCAATCTGCGCGCTCGCCTTTCGGCACGCCTCAAGGTCATGTCGCGGCTGGATATCTCCGAGCGTCGCTT
GCCGCAGGATGGCGCCATCAAGTTGCGCGTTTCGGCCTCGCGCACCATCGACTTCCGCGTCAGCACGCTGCCCACAGTAC
ACGGCGAGAAAATCGTGCTGCGCTTGCTGGACGCCGGTGCCACACGCCTGGGCATCGATGCGCTGGGCTTCAGCGAATCG
CAACAGGCGCTGTTTGAACACGCGCTCGCGCAGCCGCAGGGGATGATACTCGCCACCGGCCCCACCGGCAGCGGCAAGAC
CGTGACCCTGTATACCGGGCTCAACATCCTCAATACCGATGCGCGCAACATCGCCACCGCCGAGGACCCCGTCGAGCTCA
AGCTCGATGGCATCAATCAGGTCAATGTGCTGCCCAAGATTGGGCTGGATTTCGCCAGCGCACTGCGCGCCTTCCTGCGC
CAGGACCCCGATGTGGTGATGGTCGGTGAGGTCCGCGACCTGGAAACAGCCGAGATCTCCGTCAAGGCTGCGCAGACCGG
CCATTTGGTCCTATCGACATTGCACACGAATTCGGCGGCGGAGACCCTGACACGCCTGCGTAACATGGGTGTGGCGGCGT
ACAATATTGCAAGCTCGGTGACATTGATCGTCGCCCAGCGGTTGGTGCGCCAACTCTGCCCGCACTGCAAGACATCCGTC
GAGCTGCCTCGGGAACTCTTTTTTGAGGCAGGCTTCACGGCAGAAGAAATCGAGACCGCGACCTGCTATAGCGCCACGGG
CTGTACGCAGTGCACCCACGGCTACAAAGGACGCATCGGTCTCTATGAAGTACTCCCCGTCAGCGAGGCCATGAGCAAAC
TGATCATGGCTGACGGCAATTCATTGGAACTCGGCGAACTGGCCCGTCAAGAAGGCCATCCCGATCTGCGGCGCAGCGGT
CTGAGCAAAGTACTCGCGGGCATTACCAGCCTCGAGGAAATCAATCGCGTGGTGCTGGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-160)

Type II/IV secretion system protein (T2SSE)

(204-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

52.098

98.621

0.514
  pilB Vibrio cholerae strain A1552

50.887

97.241

0.495
  pilB Acinetobacter baylyi ADP1

49.913

98.793

0.493
  pilB Acinetobacter baumannii D1279779

48.772

98.276

0.479
  pilB Vibrio campbellii strain DS40M4

48.41

97.586

0.472
  pilB Vibrio parahaemolyticus RIMD 2210633

48.313

97.069

0.469
  pilF Neisseria gonorrhoeae MS11

45.714

96.552

0.441
  pilB/pilB1 Synechocystis sp. PCC 6803

38.953

100

0.41
  pilF Thermus thermophilus HB27

37.413

98.621

0.369