Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   U0040_RS13035 Genome accession   NZ_CP140003
Coordinates   2627806..2628927 (+) Length   373 a.a.
NCBI ID   WP_114837475.1    Uniprot ID   A0AAV3WDA8
Organism   Acinetobacter johnsonii strain HAMBI_0097     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2622806..2633927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0040_RS13020 (U0040_13020) - 2625005..2625739 (-) 735 WP_114837477.1 hypothetical protein -
  U0040_RS13025 (U0040_13025) - 2625930..2626619 (-) 690 WP_114837476.1 YggS family pyridoxal phosphate-dependent enzyme -
  U0040_RS13030 (U0040_13030) pilT 2626742..2627779 (+) 1038 WP_004694862.1 type IV pilus twitching motility protein PilT Machinery gene
  U0040_RS13035 (U0040_13035) pilU 2627806..2628927 (+) 1122 WP_114837475.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U0040_RS13040 (U0040_13040) fur 2628987..2629424 (-) 438 WP_004694866.1 ferric iron uptake transcriptional regulator -
  U0040_RS13045 (U0040_13045) - 2629537..2629935 (+) 399 WP_114837474.1 outer membrane protein assembly factor BamE -
  U0040_RS13050 (U0040_13050) - 2629982..2630305 (-) 324 WP_104222298.1 RnfH family protein -
  U0040_RS13055 (U0040_13055) - 2630305..2631387 (-) 1083 WP_114837473.1 hypothetical protein -
  U0040_RS13060 (U0040_13060) - 2631530..2631760 (-) 231 Protein_2535 hemerythrin -
  U0040_RS13065 (U0040_13065) - 2631792..2632614 (+) 823 WP_094148576.1 IS5 family transposase -
  U0040_RS13070 (U0040_13070) - 2632629..2632871 (-) 243 Protein_2537 bacteriohemerythrin -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41791.05 Da        Isoelectric Point: 6.4328

>NTDB_id=912355 U0040_RS13035 WP_114837475.1 2627806..2628927(+) (pilU) [Acinetobacter johnsonii strain HAMBI_0097]
MDFNDLLNYMIQQKASDLFITADVEPSMKINGQIVPVAKGKLTGDVAGQLLNSIMSEKQRKEFADTHECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETQIPTMDELKLPPVLKELAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGEIHEIKDLMKCSRELGMQTFDQ
ALFDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGGDRLLHASANITFDGQA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=912355 U0040_RS13035 WP_114837475.1 2627806..2628927(+) (pilU) [Acinetobacter johnsonii strain HAMBI_0097]
ATGGATTTTAACGACTTACTCAATTATATGATTCAACAAAAAGCGTCGGATTTATTTATCACCGCTGATGTTGAACCCTC
CATGAAAATTAACGGTCAAATTGTGCCTGTGGCTAAGGGCAAGCTGACGGGTGATGTCGCGGGGCAACTGCTCAACTCGA
TTATGAGTGAAAAGCAGCGTAAAGAATTTGCAGATACACATGAGTGCAACTTCGCGATTATGAATCGGGATAAAACGGCA
CGTTTTCGTGTCAGTGCCTTTCAACAGCGTGATATGCCAGGTATGGTTTTGCGTCGGATTGAGACGCAAATTCCAACCAT
GGACGAGTTAAAACTCCCGCCTGTGCTCAAAGAGTTGGCCATGACCAAACGTGGCATCATCATCTTTGTGGGAGCAACAG
GTACGGGTAAATCGACTTCTTTGGCTTCGATCATTGGTTACCGTAACCAAAACTCGAAAGGTCATATCATTACCATTGAA
GACCCAATTGAATTTATTCATGAGCATGCAGGCTGTATCATTACCCAGCGCGAAGTGGGCATTGATACGGACTCTTTTGA
AATCGCGCTGAAAAACACCTTACGTCAGGCACCCGATGTGATCCTGATTGGTGAGATTCGTTCCCGTGAAACCATGGATT
ATGCGATTGCCTTTGCTGAAACAGGTCACTTGGTATTTGCAACGCTGCATGCCAACAACGCCAACCAAGCCATCGACCGT
ATTATTCACTTCTTTGAAGCAGACCGTCATAGCCAATTGTTTATGGACTTGTCTTTAAACCTTAAAGCAATGGTTGCTCA
GCAGTTAATTCCAACACCTGACGGTAATTCACGTCGCGCTGCGATTGAGATTTTGATTAACTCACCGTTAATTTCCGATT
TGATCCGTAAAGGTGAAATCCACGAAATTAAAGACCTGATGAAGTGTTCTCGTGAACTCGGTATGCAAACTTTTGACCAA
GCCTTATTCGATTTGTACAAAGCTAAGCAGATTACCTATAAAGATGCGCTGAAACACGCCGACTCTCCAAACGACTTACG
CTTACAAATCAAGTTGTCTGAAGAAGGCGGTGATCGTCTATTGCATGCCAGTGCAAACATCACGTTTGACGGACAAGCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.72

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

68.194

99.464

0.678
  pilU Vibrio cholerae strain A1552

54.595

99.196

0.542
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.197

92.761

0.391
  pilT Legionella pneumophila strain Lp02

41.124

90.617

0.373
  pilT Legionella pneumophila strain ERS1305867

41.124

90.617

0.373
  pilT Pseudomonas aeruginosa PAK

39.017

92.761

0.362