Detailed information NTG0911207

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: U0543_RS12670
• Genome accession: NZ_CP139836
• Coordinates: 2636158..2637216 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain B84
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2631158..2642216

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U0543_RS12655 (U0543_12655)	-	2632264..2632596 (+)	333	WP_000169602.1	hypothetical protein	-
U0543_RS12660 (U0543_12660)	-	2632599..2633423 (+)	825	WP_086221410.1	putative RNA methyltransferase	-
U0543_RS12665 (U0543_12665)	ponA	2633441..2635996 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
U0543_RS12670 (U0543_12670)	pilM	2636158..2637216 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
U0543_RS12675 (U0543_12675)	pilN	2637216..2637857 (+)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
U0543_RS12680 (U0543_12680)	pilO	2637854..2638594 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
U0543_RS12685 (U0543_12685)	pilP	2638605..2639132 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
U0543_RS12690 (U0543_12690)	pilQ	2639200..2641364 (+)	2165	Protein_2479	type IV pilus secretin PilQ	-
U0543_RS12695 (U0543_12695)	aroK	2641376..2641945 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=911207 U0543_RS12670 WP_002014066.1 2636158..2637216(+) (pilM) [Acinetobacter baumannii strain B84]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=911207 U0543_RS12670 WP_002014066.1 2636158..2637216(+) (pilM) [Acinetobacter baumannii strain B84]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAACGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCCGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCATTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGATCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423