Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OCU43_RS05315 Genome accession   NZ_AP025284
Coordinates   1186348..1188066 (-) Length   572 a.a.
NCBI ID   WP_091362101.1    Uniprot ID   A0A1H9M490
Organism   Amphritea atlantica strain JCM 14776     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1181348..1193066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU43_RS05290 - 1181706..1182635 (+) 930 WP_175483622.1 Nudix family hydrolase -
  OCU43_RS05295 yacG 1183197..1183451 (-) 255 WP_175483623.1 DNA gyrase inhibitor YacG -
  OCU43_RS05300 coaE 1183444..1184061 (-) 618 WP_091362098.1 dephospho-CoA kinase -
  OCU43_RS05305 pilD 1184117..1185016 (-) 900 WP_091362099.1 A24 family peptidase Machinery gene
  OCU43_RS05310 pilC 1185054..1186283 (-) 1230 WP_091362100.1 type II secretion system F family protein Machinery gene
  OCU43_RS05315 pilB 1186348..1188066 (-) 1719 WP_091362101.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCU43_RS05320 - 1188494..1188919 (+) 426 WP_091362102.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OCU43_RS05325 - 1189058..1189417 (+) 360 WP_091362103.1 hypothetical protein -
  OCU43_RS05330 nadC 1189839..1190684 (-) 846 WP_091362104.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OCU43_RS05335 ampD 1190862..1191440 (+) 579 WP_091362105.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OCU43_RS05340 - 1191437..1192285 (+) 849 WP_091362106.1 hypothetical protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 63121.31 Da        Isoelectric Point: 5.1551

>NTDB_id=91040 OCU43_RS05315 WP_091362101.1 1186348..1188066(-) (pilB) [Amphritea atlantica strain JCM 14776]
MSVQPFQPLSGLARRLVNDGVFSSDVAMDALKTARKDNKPFIAHIIENRLVSAYRAAAAAADEFGMPLFDLDVIDIEALP
QGLVDEKLITKHHALPLIKRENRLYVAISDPTNIQALDEFKFHTGTTTEGVIVEEDKLAHLIDTFLDEQNSALDGLEDAD
LDNLEIDEGLNEQQEDNGEEAANDAPIVRFVNKILLDAIKTGASDIHFEPYEKSYRIRCRIDGILQEVAKPPANLASRLC
ARLKVMSQMDISERRVPQDGRIKMKISKNRAIDFRVNTLPTLWGEKVVLRILDPTSAQMGIEALGYDEDQKELFMQTLHK
PQGMILVTGPTGSGKTVSLYTGLNILNTVERNISTAEDPVEINLEGINQVHVNNKVGLTFAEALRSFLRQDPDVVMVGEI
RDLETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLRNMGVPAFNIATSVSMIIAQRLGRRLCSACKKPATIPEATLLEEG
FTPEQIKAGLSLFEHNPEGCSKCNKGYKGRVGVYETMAMTPEIAQVIMDNGSSLDILATAKQQGFRSLRQSGLRKVIQGV
TSLEEMNRVIKL

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=91040 OCU43_RS05315 WP_091362101.1 1186348..1188066(-) (pilB) [Amphritea atlantica strain JCM 14776]
ATGAGCGTACAACCCTTTCAGCCGCTGAGTGGTCTGGCACGACGACTGGTTAATGACGGTGTGTTTTCATCCGATGTTGC
CATGGACGCCCTGAAAACCGCCCGCAAGGACAATAAGCCCTTTATCGCCCATATTATCGAAAACCGCCTGGTGAGTGCCT
ATCGGGCTGCTGCGGCCGCTGCCGATGAGTTCGGCATGCCCCTGTTTGACCTCGATGTCATCGATATTGAAGCGCTGCCC
CAGGGCCTGGTGGATGAAAAGCTAATCACCAAACACCATGCCCTGCCTCTGATCAAGCGTGAGAACCGCCTCTATGTGGC
AATCTCAGACCCTACCAATATTCAGGCGCTGGATGAGTTTAAGTTCCATACCGGCACCACCACCGAGGGGGTGATCGTTG
AAGAGGATAAGCTCGCCCATCTGATCGACACCTTCCTCGATGAACAGAACTCCGCCCTCGATGGCCTGGAAGATGCCGAT
CTCGATAACCTGGAGATCGATGAGGGGCTTAATGAGCAGCAGGAAGACAATGGTGAAGAAGCCGCCAACGATGCTCCGAT
TGTACGCTTCGTCAACAAAATACTGCTGGATGCGATAAAAACCGGTGCTTCGGATATCCACTTCGAGCCCTATGAGAAAT
CCTACCGCATCCGCTGCCGTATCGACGGCATTCTGCAGGAGGTCGCCAAGCCCCCAGCCAATCTGGCTTCCCGTCTCTGC
GCCCGACTGAAAGTGATGTCGCAGATGGATATCTCCGAGCGACGAGTCCCCCAGGATGGCCGTATCAAGATGAAGATCTC
CAAAAACCGCGCCATCGACTTCCGGGTTAACACCCTGCCCACCCTCTGGGGCGAAAAAGTGGTGCTGCGTATTCTCGACC
CCACCAGTGCGCAGATGGGGATCGAAGCGCTGGGTTACGATGAAGACCAGAAAGAGCTGTTTATGCAAACCCTGCATAAA
CCACAAGGGATGATTCTGGTGACCGGCCCCACCGGTAGCGGTAAAACCGTCTCCCTCTATACCGGCCTGAATATTCTCAA
CACCGTTGAACGCAATATATCCACCGCGGAGGACCCGGTAGAGATCAACCTGGAGGGGATCAACCAGGTCCATGTGAATA
ATAAAGTGGGACTGACCTTCGCAGAAGCACTGCGATCTTTCCTGCGACAAGATCCGGATGTAGTAATGGTAGGTGAGATC
CGAGATCTGGAAACCGCTGAGATCGCTATCAAAGCCGCCCAGACCGGCCATATGGTATTGTCAACTCTGCACACCAACAG
TGCCGCCGAAACACTCACACGACTACGCAATATGGGGGTTCCGGCCTTTAATATTGCTACCTCTGTGTCTATGATTATTG
CCCAGCGACTGGGACGACGTCTGTGCAGCGCGTGTAAAAAGCCCGCCACCATTCCTGAAGCAACATTACTGGAAGAGGGC
TTTACTCCGGAACAGATAAAAGCGGGTCTCAGTCTGTTTGAGCATAATCCGGAAGGGTGCAGTAAGTGCAATAAGGGCTA
TAAAGGCCGGGTGGGGGTCTATGAAACCATGGCGATGACCCCGGAGATTGCCCAGGTGATTATGGACAACGGTTCATCTC
TGGATATTCTGGCAACCGCAAAACAGCAGGGGTTCCGCTCTCTCAGACAGTCAGGCCTGCGCAAAGTAATTCAGGGCGTC
ACCAGCCTGGAAGAGATGAACCGGGTTATAAAACTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H9M490

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.566

98.252

0.605
  pilB Acinetobacter baylyi ADP1

60.391

98.427

0.594
  pilB Legionella pneumophila strain ERS1305867

55.752

98.776

0.551
  pilB Vibrio cholerae strain A1552

55.361

89.685

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

53.516

89.51

0.479
  pilF Neisseria gonorrhoeae MS11

48.49

98.427

0.477
  pilB Vibrio campbellii strain DS40M4

52.827

89.685

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

40.388

90.035

0.364

Multiple sequence alignment