Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   OCU43_RS05305 Genome accession   NZ_AP025284
Coordinates   1184117..1185016 (-) Length   299 a.a.
NCBI ID   WP_091362099.1    Uniprot ID   A0A1H9M2M7
Organism   Amphritea atlantica strain JCM 14776     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1179117..1190016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU43_RS05285 argJ 1180458..1181675 (+) 1218 WP_091362096.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  OCU43_RS05290 - 1181706..1182635 (+) 930 WP_175483622.1 Nudix family hydrolase -
  OCU43_RS05295 yacG 1183197..1183451 (-) 255 WP_175483623.1 DNA gyrase inhibitor YacG -
  OCU43_RS05300 coaE 1183444..1184061 (-) 618 WP_091362098.1 dephospho-CoA kinase -
  OCU43_RS05305 pilD 1184117..1185016 (-) 900 WP_091362099.1 A24 family peptidase Machinery gene
  OCU43_RS05310 pilC 1185054..1186283 (-) 1230 WP_091362100.1 type II secretion system F family protein Machinery gene
  OCU43_RS05315 pilB 1186348..1188066 (-) 1719 WP_091362101.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCU43_RS05320 - 1188494..1188919 (+) 426 WP_091362102.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OCU43_RS05325 - 1189058..1189417 (+) 360 WP_091362103.1 hypothetical protein -

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 33008.89 Da        Isoelectric Point: 4.6597

>NTDB_id=91038 OCU43_RS05305 WP_091362099.1 1184117..1185016(-) (pilD) [Amphritea atlantica strain JCM 14776]
MMFELIAADTWLSLSLTFVFALMVGSFLNVVIHRLPQMMEREWQLMAAEAAEAAETRDPEAQETPAEPSPPFNLAQPAST
CPNCGHLIHWYENIPLISYFLILGGKCSGCKTRISLRYPLIELVTALISAFIVWQFGFNPVAFAAIFFSWCLIALTAIDI
DHQLLPDKITLPLLWIGLIINSFGYFTTLPSALWGAVLGYLSLWSVYWLFKLATGKEGMGYGDFKLLAALGAWAGADMLP
LIILASSLIGAIIGGIMIAFSRQQSQTPIPFGPYLAGAGWIALLWGDELTSWYLHLLNL

Nucleotide


Download         Length: 900 bp        

>NTDB_id=91038 OCU43_RS05305 WP_091362099.1 1184117..1185016(-) (pilD) [Amphritea atlantica strain JCM 14776]
ATGATGTTCGAACTGATTGCAGCTGATACCTGGTTGTCGCTGTCACTTACCTTTGTTTTTGCACTGATGGTGGGCAGTTT
TCTGAATGTGGTTATTCACAGACTGCCACAGATGATGGAGCGGGAATGGCAGTTGATGGCAGCTGAAGCTGCTGAAGCTG
CTGAAACAAGAGATCCGGAGGCACAGGAAACACCCGCAGAGCCATCCCCCCCCTTCAACCTGGCTCAACCTGCATCAACC
TGCCCTAACTGCGGACATCTGATCCACTGGTATGAAAATATTCCGCTAATTAGCTACTTTTTGATTCTGGGTGGAAAATG
CAGTGGTTGTAAAACCAGGATATCACTGCGCTATCCCCTTATTGAACTGGTCACTGCGCTGATATCGGCTTTTATTGTCT
GGCAGTTCGGCTTTAACCCGGTGGCTTTTGCCGCGATCTTCTTCAGCTGGTGTCTGATTGCGCTTACCGCTATCGATATT
GATCATCAGCTATTGCCGGATAAAATCACCCTGCCGCTGCTCTGGATCGGCCTGATCATCAATAGCTTTGGTTACTTCAC
CACGCTGCCCTCAGCCCTGTGGGGAGCGGTGCTGGGCTACCTCTCCCTCTGGTCGGTTTACTGGCTATTCAAACTGGCCA
CCGGCAAAGAGGGGATGGGCTATGGCGATTTCAAGCTGCTTGCGGCACTGGGAGCCTGGGCCGGCGCCGATATGCTACCA
CTGATTATTCTGGCCTCCTCACTGATCGGCGCTATTATCGGTGGGATCATGATCGCTTTCAGCCGTCAGCAATCGCAAAC
ACCAATCCCTTTTGGCCCCTATCTGGCAGGCGCAGGCTGGATCGCGCTGTTATGGGGAGATGAGCTGACCAGCTGGTATC
TGCATCTGCTAAACTTATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(147-254)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(19-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H9M2M7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

54.296

97.324

0.528
  pilD Acinetobacter nosocomialis M2

53.65

91.639

0.492
  pilD Acinetobacter baumannii D1279779

53.285

91.639

0.488
  pilD Vibrio campbellii strain DS40M4

50.172

97.324

0.488
  pilD Neisseria gonorrhoeae MS11

45.965

95.318

0.438

Multiple sequence alignment