Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SOI81_RS15770 Genome accession   NZ_CP139286
Coordinates   3347938..3349650 (+) Length   570 a.a.
NCBI ID   WP_320540985.1    Uniprot ID   -
Organism   Acinetobacter pittii strain AbaK     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3342938..3354650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SOI81_RS15735 (SOI81_15735) nusA 3343827..3345311 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  SOI81_RS15740 (SOI81_15740) rimP 3345348..3345872 (-) 525 WP_016142372.1 ribosome maturation factor RimP -
  SOI81_RS15760 (SOI81_15760) secG 3346510..3346839 (-) 330 WP_016142373.1 preprotein translocase subunit SecG -
  SOI81_RS15765 (SOI81_15765) tpiA 3346852..3347646 (-) 795 WP_016142374.1 triose-phosphate isomerase -
  SOI81_RS15770 (SOI81_15770) pilB 3347938..3349650 (+) 1713 WP_320540985.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SOI81_RS15775 (SOI81_15775) pilC 3349678..3350904 (+) 1227 WP_320540986.1 type II secretion system F family protein Machinery gene
  SOI81_RS15780 (SOI81_15780) pilD 3350904..3351764 (+) 861 WP_320540987.1 A24 family peptidase Machinery gene
  SOI81_RS15785 (SOI81_15785) coaE 3351766..3352362 (+) 597 WP_062849621.1 dephospho-CoA kinase -
  SOI81_RS15790 (SOI81_15790) - 3352359..3353273 (-) 915 WP_016142379.1 DMT family transporter -
  SOI81_RS15795 (SOI81_15795) rlmB 3353309..3354058 (-) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  SOI81_RS15800 (SOI81_15800) - 3354161..3354487 (-) 327 WP_004795031.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63625.14 Da        Isoelectric Point: 5.3425

>NTDB_id=908772 SOI81_RS15770 WP_320540985.1 3347938..3349650(+) (pilB) [Acinetobacter pittii strain AbaK]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKNLVDQKLILKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILTQQFTEESSFEFSDEEF
DLDVDLEAPSSQEEEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKIPADIPKQSLLEMGF
TKQDLTHPDFQVFQPIGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=908772 SOI81_RS15770 WP_320540985.1 3347938..3349650(+) (pilB) [Acinetobacter pittii strain AbaK]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCGTTAGATGCAGCAAAAAGAGCTAAACGGGATACGGTTGCGTACCTAATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACTATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACCAGCCAAATT
CCTAAAAATTTGGTTGACCAGAAACTGATTTTAAAACATCGGGTCTTGCCTCTTATACAAAGAGGACAGATTTTATATGT
CGCGACCAGCAATCCGAGTAATATTGAAGCAATTGATGCGATTCGCTTCAATAGCAAGCTGATGGTTGAGCCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGACGCAGCAATTTACCGAAGAAAGCAGTTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCATCTTCACAAGAAGAGGAGGAAGAAACACCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATAGATGCGATTCGTATGGGAGCTTCAGACTTACATTTTGAACCCTATGAGAAATCAT
ATCGGGTTCGTTACCGAGTCGATGGTGTATTACGGCAGATTGCAAATCCGCCTTTACAGTTGGCTAACCGTTTAGCTTCA
CGTTTGAAAGTCATGTCTCAAATGGACATTTCCGAAAAACGGGTGCCTCAAGATGGTCGTATCAAACTTAAGCTCTCAAA
AACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACCTTGTTTGGCGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCGATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTGGATAAACCA
CAAGGCATGCTTTTAATTACAGGTCCTACAGGTTCCGGTAAAACGGTATCTTTATATACGGGCTTAAATATTTTAAATAC
TGAAAGTTCGAATATTTCCACCGCCGAAGATCCAGTTGAAATTAACCTTGAAGGAATTAATCAGGTTAACGTCAACCCGA
AAGTAGGTCTAACTTTTTCTGCTGCACTTAAATCATTTTTACGTCAAGATCCGGACATTATTATGGTCGGTGAGATTCGT
GATTTAGAAACGGCTGAAATTGCGATTAAGGCTGCACAAACTGGTCACATGGTCATGTCCACACTGCATACCAATAGTGC
TCCAGAAACATTGACTCGCCTACGCAATATGGGTGTTCCATCCTTTAATATCGCAACATCAGTCAACTTGGTTATTGCAC
AGCGTTTAGCACGTCGGCTATGCCCACAGTGTAAAATACCTGCTGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCAAACAAGATCTGACTCATCCCGATTTTCAAGTTTTCCAACCTATCGGTTGTACTGAGTGTCGTGAAGGCTATAAAGG
TCGAGTAGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGATTAAAGAAAGTCATGCAAGGTGTTACTTCT
TTACAAGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.737

100

0.947
  pilB Acinetobacter baylyi ADP1

77.719

100

0.777
  pilB Legionella pneumophila strain ERS1305867

51.323

99.474

0.511
  pilF Neisseria gonorrhoeae MS11

49.288

98.596

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

51.321

92.982

0.477
  pilB Vibrio campbellii strain DS40M4

51.06

91.053

0.465
  pilB Vibrio cholerae strain A1552

50.482

91.053

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384