Detailed information NTG0908623

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SOI77_RS14960
• Genome accession: NZ_CP139275
• Coordinates: 3164460..3165578 (+)
• Length: 372 a.a.
• NCBI ID: WP_017398880.1
• Uniprot ID: A0A6H0FWV9
• Organism: Acinetobacter pittii strain HCG138
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3159460..3170578

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SOI77_RS14950 (SOI77_14950)	yggS	3162584..3163276 (-)	693	WP_304595400.1	YggS family pyridoxal phosphate-dependent enzyme	-
SOI77_RS14955 (SOI77_14955)	pilT	3163403..3164440 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
SOI77_RS14960 (SOI77_14960)	pilU	3164460..3165578 (+)	1119	WP_017398880.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SOI77_RS14965 (SOI77_14965)	fur	3165648..3166085 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
SOI77_RS14970 (SOI77_14970)	omlA	3166197..3166595 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
SOI77_RS14975 (SOI77_14975)	-	3166660..3166974 (-)	315	WP_057068397.1	RnfH family protein	-
SOI77_RS14980 (SOI77_14980)	-	3166974..3168041 (-)	1068	WP_139846616.1	hypothetical protein	-
SOI77_RS14985 (SOI77_14985)	-	3168195..3168650 (-)	456	WP_002121698.1	bacteriohemerythrin	-
SOI77_RS14990 (SOI77_14990)	-	3168902..3169750 (-)	849	WP_014205980.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41917.12 Da
• Isoelectric Point: 6.6404

>NTDB_id=908623 SOI77_RS14960 WP_017398880.1 3164460..3165578(+) (pilU) [Acinetobacter pittii strain HCG138]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKSA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=908623 SOI77_RS14960 WP_017398880.1 3164460..3165578(+) (pilU) [Acinetobacter pittii strain HCG138]
ATGGATTTTAACGACCTGCTCAACCTCATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGGCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAGACTCGTGAATGTAATTTTGCCATTATGAACCGCGATAAAAGCGCG
CGCTTTCGTGTAAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTACTACGTCGAATTGAAACCAAAATTCCTTCAAT
TGATGACTTGCAATTACCGCCTGTACTAAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACACGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATTGATACAGACTCATTTGA
AATTGCTTTAAAGAATACGTTGCGACAAGCACCCGATGTGATCTTAATTGGTGAGATTCGCTCTCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACGGGTCACTTGGTATTAGCCACAATGCATGCGAACAATGCCAACCAAGCACTTGATCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCTCGCCGTGCAGCGATTGAGATTCTCATTAACTCTCCATTAATTTCAGACT
ATATTCGTAAAGGTGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A6H0FWV9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.733	99.731	0.685
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363