Detailed information NTG0908340

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SOI71_RS13895
• Genome accession: NZ_CP139251
• Coordinates: 2935494..2936612 (+)
• Length: 372 a.a.
• NCBI ID: WP_002121759.1
• Uniprot ID: A0A022J839
• Organism: Acinetobacter pittii strain MCR53
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2930494..2941612

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SOI71_RS13885 (SOI71_13875)	yggS	2933618..2934310 (-)	693	WP_104075094.1	YggS family pyridoxal phosphate-dependent enzyme	-
SOI71_RS13890 (SOI71_13880)	pilT	2934437..2935474 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
SOI71_RS13895 (SOI71_13885)	pilU	2935494..2936612 (+)	1119	WP_002121759.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SOI71_RS13900 (SOI71_13890)	fur	2936682..2937119 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
SOI71_RS13905 (SOI71_13895)	omlA	2937231..2937629 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
SOI71_RS13910 (SOI71_13900)	-	2937694..2938008 (-)	315	WP_017481335.1	RnfH family protein	-
SOI71_RS13915 (SOI71_13905)	-	2938008..2939075 (-)	1068	WP_104075093.1	hypothetical protein	-
SOI71_RS13920 (SOI71_13910)	-	2939229..2939684 (-)	456	WP_002121698.1	bacteriohemerythrin	-
SOI71_RS13925 (SOI71_13915)	-	2939936..2940784 (-)	849	WP_014205980.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41945.18 Da
• Isoelectric Point: 6.6404

>NTDB_id=908340 SOI71_RS13895 WP_002121759.1 2935494..2936612(+) (pilU) [Acinetobacter pittii strain MCR53]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRTREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=908340 SOI71_RS13895 WP_002121759.1 2935494..2936612(+) (pilU) [Acinetobacter pittii strain MCR53]
ATGGATTTTAACGACCTGCTCAACCTCATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGGCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAGACTCGTGAATGTAATTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTATTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAATTACCACCTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCTTTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCGATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCGTTCGA
AATTGCCTTAAAGAATACGTTACGACAAGCACCCGATGTCATCTTGATTGGTGAAATTCGTACGCGTGAAGTGATGGACT
ATGCAATTGGCTTCGCCGAAACTGGCCACTTGGTCTTAGCCACAATGCACGCCAACAATGCCAACCAAGCGCTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCCCCATTAATTTCAGACT
ATATTCGTAAAGGTGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

3D structure

• AlphaFold DB: A0A022J839

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	69.003	99.731	0.688
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363