Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SJN48_RS18115 Genome accession   NZ_CP139212
Coordinates   3820861..3822573 (+) Length   570 a.a.
NCBI ID   WP_130120471.1    Uniprot ID   -
Organism   Acinetobacter pittii strain WWTP     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3815861..3827573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SJN48_RS18080 (SJN48_18120) nusA 3816749..3818233 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  SJN48_RS18085 (SJN48_18125) rimP 3818271..3818795 (-) 525 WP_002114922.1 ribosome maturation factor RimP -
  SJN48_RS18105 (SJN48_18145) secG 3819433..3819762 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  SJN48_RS18110 (SJN48_18150) tpiA 3819775..3820569 (-) 795 WP_005079338.1 triose-phosphate isomerase -
  SJN48_RS18115 (SJN48_18155) pilB 3820861..3822573 (+) 1713 WP_130120471.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SJN48_RS18120 (SJN48_18160) pilC 3822600..3823826 (+) 1227 WP_119684816.1 type II secretion system F family protein Machinery gene
  SJN48_RS18125 (SJN48_18165) pilD 3823826..3824686 (+) 861 WP_002114758.1 A24 family peptidase Machinery gene
  SJN48_RS18130 (SJN48_18170) coaE 3824688..3825284 (+) 597 WP_130120472.1 dephospho-CoA kinase -
  SJN48_RS18135 (SJN48_18175) - 3825281..3826195 (-) 915 WP_046812059.1 DMT family transporter -
  SJN48_RS18140 (SJN48_18180) rlmB 3826231..3826980 (-) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  SJN48_RS18145 (SJN48_18185) - 3827083..3827409 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63580.95 Da        Isoelectric Point: 5.0911

>NTDB_id=908127 SJN48_RS18115 WP_130120471.1 3820861..3822573(+) (pilB) [Acinetobacter pittii strain WWTP]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKKAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILTQQFTEESNFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKTPADIPKQSLLEMGF
NDQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=908127 SJN48_RS18115 WP_130120471.1 3820861..3822573(+) (pilB) [Acinetobacter pittii strain WWTP]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAAAGCTAAACGGGATACAGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACAAGCCAAATT
CCCAAAGATTTGGTCGATCAGAAACTGGTTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCAACCAGCAACCCGAGTAATATTGAAGCGATTGATGCCATTCGCTTTAATAGCAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGACGCAGCAATTTACCGAAGAAAGTAACTTCGAATTTAGTGATGAAGAATTT
GATCTTGATGTAGACCTTGAAGCTCCACCCTCACAAGAGGATGAGGAAGAAACACCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCGATTCGTATGGGGGCTTCAGACTTACATTTTGAACCCTATGAGAAATCAT
ATCGGGTCCGTTATCGAGTCGATGGTGTATTGCGCCAGATCGCAAATCCGCCTTTACAGTTGGCTAACCGTTTAGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAGCGGGTACCTCAAGATGGCCGTATCAAGCTGAAGTTATCAAA
AACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTACGTATTCTCGATCCAT
CTAGCGCAATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATCACAGGTCCTACCGGTTCTGGTAAAACGGTATCTTTATATACTGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGCATTAATCAGGTTAACGTTAACCCGA
AAGTAGGTCTTACTTTTTCGGCCGCACTGAAATCATTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTGGAAACAGCAGAGATTGCGATTAAAGCTGCACAAACCGGTCACATGGTGATGTCAACGCTACATACCAATAGTGC
CCCGGAAACTCTGACTCGTCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACGTCTGTCAATCTGGTGATTGCTC
AACGTTTGGCGCGTCGGTTATGCCCACAGTGTAAAACACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
AACGACCAAGATTTAACCCATCCCGATTTTCAAGTTTTTCAACCTGTAGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTAGGTATTTATGAGGTGATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGATTAAAGAAAGTCATGCAAGGTGTAACTTCC
TTACAAGAAGTTAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.386

100

0.944
  pilB Acinetobacter baylyi ADP1

78.07

100

0.781
  pilB Legionella pneumophila strain ERS1305867

51.146

99.474

0.509
  pilF Neisseria gonorrhoeae MS11

49.644

98.596

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

51.321

92.982

0.477
  pilB Vibrio campbellii strain DS40M4

50.766

91.579

0.465
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379