Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   SHB59_RS18975 Genome accession   NZ_CP139026
Coordinates   4266169..4268241 (-) Length   690 a.a.
NCBI ID   WP_085533526.1    Uniprot ID   -
Organism   Pseudomonas sp. S1Bt23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4261169..4273241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHB59_RS18960 (SHB59_18960) - 4262797..4264386 (-) 1590 WP_085533530.1 SPOR domain-containing protein -
  SHB59_RS18965 (SHB59_18965) aroB 4264397..4265497 (-) 1101 WP_092419023.1 3-dehydroquinate synthase -
  SHB59_RS18970 (SHB59_18970) aroK 4265646..4266164 (-) 519 WP_009041824.1 shikimate kinase AroK -
  SHB59_RS18975 (SHB59_18975) pilQ 4266169..4268241 (-) 2073 WP_085533526.1 type IV pilus secretin PilQ Machinery gene
  SHB59_RS18980 (SHB59_18980) - 4268255..4268782 (-) 528 WP_320448337.1 pilus assembly protein PilP -
  SHB59_RS18985 (SHB59_18985) - 4268779..4269396 (-) 618 WP_320448338.1 type 4a pilus biogenesis protein PilO -
  SHB59_RS18990 (SHB59_18990) - 4269393..4269959 (-) 567 WP_009041820.1 PilN domain-containing protein -
  SHB59_RS18995 (SHB59_18995) pilM 4269959..4271011 (-) 1053 WP_320448339.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74742.90 Da        Isoelectric Point: 5.2124

>NTDB_id=906816 SHB59_RS18975 WP_085533526.1 4266169..4268241(-) (pilQ) [Pseudomonas sp. S1Bt23]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLADKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAALSAPGQPAVASGRAKAVASVGRSIRSVDFQ
RGEQGEGNVLIDLSDSEMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNAERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGTNG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=906816 SHB59_RS18975 WP_085533526.1 4266169..4268241(-) (pilQ) [Pseudomonas sp. S1Bt23]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGTTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAGCTGACCTTCGATGGACCGGTGCCCGAGCCGCGCG
GCTATACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCCGGTGTCGTCAGCCAGCTGGCAGACAAGAGCCGTGAC
CTGGGCAATGGCAATGCGCGCAGTGTGACGGTGGTCGAATCGGGGCAGCGTACCCGGGTTGTCGTCAATCTGGTCGCGCT
GGCGCCCTACAGCACTCGGGTCGCTGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCTGAGTGCGC
CGGGGCAGCCGGCTGTCGCGTCGGGGCGGGCCAAGGCCGTGGCTTCCGTGGGGCGGTCGATTCGCAGCGTGGATTTCCAG
CGCGGCGAGCAGGGCGAGGGGAATGTACTGATCGACCTCTCCGACTCGGAAATGAGCCCGGACATACAGGAGCGCGACGG
CAAGCTCGTCCTCACCTTCGCCAAGGCCCGCCTGCCCGAGCCCCTGCGGGTCAGGCTGGACGTCAAGGATTTCGCCACCC
CCGTGCAGTTCGTCAGCGCCCGGGCAGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACTTTCGATTACTCG
ACCTACCAGACGGATAACAAACTCACGGTGAGCGTCAGGCCGATGACCTCTGGCGACCTGCAGAAACGCAACGCCGAGCG
CTCGGCCTACAACGGCGAAAAGCTCTCGTTGAACTTCCAGGACATCGAGGTTCGCTCGGTGCTGCAACTGATCGCCGATT
TCACCAACCTCAACCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCCCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAACGTGTTGCTGGTGGCGCCGGCCGATGA
AATCGCTGCCCGCGAACGCCAGGAACTGGAGTCGCAGAAGCAGATCGCCGACCTGGAACCATTGCGGCGCGAACTGCTGC
AGGTCAACTACGCCAAGGCCGCGGAGATCGCCAAGTTGTTCCAGTCGGTCACCCGCGCCGAGGACAAGTCCGACGAGCGC
GGGTCGATCACTGTCGATGAGCGGACCAACAACATCATCGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
CGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAATCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCACCAACGGT
TCCTCCACCATAGGCACGCCGGGCAGCACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACTACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAATGTGTTGCTGGATCTTGAGCTGACAGCCATGGAAAAGACCGGTAACGGGGAAA
TCGTCTCCCAGCCCAAGGTCGTCACGTCCGACAAGGAGACGGCGAAGATCCTCAAGGGGACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGCGGGGCGACCTCGGTGTCGTTCAAGGAGGCCTCGCTGTCCCTGGAGGTGACGCCACAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAAGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGATGGAGAGACCATCGTCATCGGTGGAGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

AMIN domain (AMIN)

(40-122)

Secretin and TonB N terminus short domain (STN)

(298-344)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.769

100

0.733
  comQ Acinetobacter baylyi ADP1

40.055

100

0.423
  pilQ Acinetobacter baumannii strain A118

38.121

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.121

100

0.412
  pilQ Legionella pneumophila strain Lp02

38.039

97.536

0.371
  pilQ Legionella pneumophila strain ERS1305867

38.117

96.957

0.37