Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   SHB59_RS18995 Genome accession   NZ_CP139026
Coordinates   4269959..4271011 (-) Length   350 a.a.
NCBI ID   WP_320448339.1    Uniprot ID   -
Organism   Pseudomonas sp. S1Bt23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4264959..4276011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHB59_RS18970 (SHB59_18970) aroK 4265646..4266164 (-) 519 WP_009041824.1 shikimate kinase AroK -
  SHB59_RS18975 (SHB59_18975) pilQ 4266169..4268241 (-) 2073 WP_085533526.1 type IV pilus secretin PilQ Machinery gene
  SHB59_RS18980 (SHB59_18980) - 4268255..4268782 (-) 528 WP_320448337.1 pilus assembly protein PilP -
  SHB59_RS18985 (SHB59_18985) - 4268779..4269396 (-) 618 WP_320448338.1 type 4a pilus biogenesis protein PilO -
  SHB59_RS18990 (SHB59_18990) - 4269393..4269959 (-) 567 WP_009041820.1 PilN domain-containing protein -
  SHB59_RS18995 (SHB59_18995) pilM 4269959..4271011 (-) 1053 WP_320448339.1 pilus assembly protein PilM Machinery gene
  SHB59_RS19000 (SHB59_19000) - 4271228..4273662 (+) 2435 Protein_3762 penicillin-binding protein 1A -
  SHB59_RS19005 (SHB59_19005) - 4273803..4275071 (-) 1269 WP_062823907.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37097.47 Da        Isoelectric Point: 5.1051

>NTDB_id=906817 SHB59_RS18995 WP_320448339.1 4269959..4271011(-) (pilM) [Pseudomonas sp. S1Bt23]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLSDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGSPTARTVALVDIGATLTTLSVVHEGQIVYSREQLFGGHQLTEHIQRRYGLSKEAAG
QAKKQGGLPDDYTSEVLQPFQEAVVQQVSRSLQFFQASAPQHQVDSILLAGGSASVPGLVPLIEQRLGLPALIANPFVGM
SIGARVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=906817 SHB59_RS18995 WP_320448339.1 4269959..4271011(-) (pilM) [Pseudomonas sp. S1Bt23]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAATT
GAGCCGTGTCGGTGGCCGCTTTCGGGTCGAGGCCTATGGCGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCTGAAGGGGTCGGGCATGCCCTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGTGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTGTCCGACGACGATCGGGAAAGCCAGCT
CAAGGTCGACGCCGACCAGTACATTCCCTACCCGCTGGACGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCCC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAGCACATCGAAGTTCGTGAGGCGGCGCTCGAACTT
GCGGGCCTGACTGCGCGGGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGTTCGTTCGCCCTGGCGGCCGGCTCGCCGAC
TGCCCGGACCGTGGCGCTGGTCGATATCGGCGCCACCCTCACCACCCTGAGCGTCGTGCATGAAGGGCAGATCGTCTATT
CCCGTGAGCAGTTGTTCGGCGGACACCAACTGACCGAACACATCCAGCGTCGTTACGGGCTGTCGAAAGAAGCGGCCGGC
CAGGCAAAAAAGCAGGGCGGCCTGCCGGACGATTACACCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAACA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGCCCCGCAACACCAGGTCGATTCCATCCTGTTGGCGGGTGGCAGCG
CCTCGGTTCCGGGGCTTGTCCCGTTGATCGAGCAGCGCCTGGGCTTGCCTGCGCTGATCGCCAACCCCTTCGTTGGCATG
AGCATCGGCGCCAGGGTCGACGCCGCGGCCCTGGCCAGCGAGGCCCCATCCTTGATGATCGCCTGCGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.114

100

0.554
  comM Acinetobacter nosocomialis M2

54.83

100

0.551
  comM Acinetobacter baylyi ADP1

52.273

100

0.526
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.454