Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   R6238_RS12390 Genome accession   NZ_CP138212
Coordinates   2932099..2934204 (-) Length   701 a.a.
NCBI ID   WP_024959592.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. syringae strain pss-2019     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2927099..2939204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6238_RS12375 - 2928730..2930346 (-) 1617 WP_003316656.1 SPOR domain-containing protein -
  R6238_RS12380 aroB 2930357..2931460 (-) 1104 WP_003422530.1 3-dehydroquinate synthase -
  R6238_RS12385 aroK 2931576..2932094 (-) 519 WP_003341387.1 shikimate kinase AroK -
  R6238_RS12390 pilQ 2932099..2934204 (-) 2106 WP_024959592.1 type IV pilus secretin PilQ Machinery gene
  R6238_RS12395 pilP 2934252..2934779 (-) 528 WP_003316653.1 type 4a pilus biogenesis lipoprotein PilP -
  R6238_RS12400 pilO 2934776..2935399 (-) 624 WP_003316652.1 type 4a pilus biogenesis protein PilO -
  R6238_RS12405 - 2935396..2935977 (-) 582 WP_024959591.1 PilN domain-containing protein -
  R6238_RS12410 pilM 2935977..2937041 (-) 1065 WP_003341394.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75444.80 Da        Isoelectric Point: 5.1826

>NTDB_id=901442 R6238_RS12390 WP_024959592.1 2932099..2934204(-) (pilQ) [Pseudomonas syringae pv. syringae strain pss-2019]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTSQPARIALDLPGVTSQLATKSRD
LGSGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAGAAQASQPTTAMGAQRVAVPPPTPASARPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASASGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDVDRRAADKPAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGNR
KWTTYGLDNNGDEAGNTSGNLTSNVPFVDLGAAGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=901442 R6238_RS12390 WP_024959592.1 2932099..2934204(-) (pilQ) [Pseudomonas syringae pv. syringae strain pss-2019]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCAGCCCTGCCGGGCGATCGCATCGAGCTGAAGCTGGCATTCGATGCCCCGGTGCCTGCACCGCGCG
GTTACACCACGTCGCAACCGGCGCGCATTGCGCTGGATCTGCCGGGTGTTACCAGCCAGCTGGCGACCAAAAGTCGTGAT
CTGGGCAGCGGCAATGCGCGCAGTGTGGTTGTGGTTCAGGCTCAGGACCGCACTCGGGTGATCATCAACCTGACCACGCT
TTCGCCTTACAGCTCCCGCGTCGATGGCAACAACCTGTTCGTGGTCATCGGCCAGGGTGCCGGCGCCGCGCAAGCCTCCC
AGCCGACCACTGCGATGGGCGCGCAACGGGTCGCGGTGCCGCCGCCCACGCCGGCATCCGCCAGGCCGTTCATCCCCGCT
GGCGTCAAGGCCATCAGGAATGTCGATTTCCAGCGTGGCGAGCTGGGTGAAGGCAATGTGGTCATCGACCTGAGCAACCC
CAGCATTGCCCCGGATATTCAGGAGCAGGGCGGCAAGATCCGCGTGGACTTCGCCAAGACCCAACTGCCCGAACCGCTGC
GCGTCCGCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAGTGCTTCGGCGTCGGGTGACAAGGCCAGCATT
CTGATCGAGCCGTCGGGCGCCTTCGACTACTCGGCGTTTCAGACCGAAAACAAGCTGACGATCAGCGTGCGTCCATTGAC
CAACGAGGACGTCGACCGCCGCGCCGCTGACAAACCGGCGTACACCGGTGAAAAGCTGTCGCTGAACTTCCAGGACATCG
ACGTGCGTTCGGTGCTGCAGCTGATTGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTCCAGGGTGGTATC
ACTCTGCGTCTGCAGAATGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAACGCAAGGT
CGGCAACGTGCTGCTGGTTGCGCCAGCCGATGAAATTGCCGCCCGCGAGCGTCAGGAGCTGGAGTCGCTCAAGCAGATCG
CCGAGCTGGCGCCCCTGCGCCGTGAGCTGTTGCAGGTCAATTACGCCAAGGCTGCCGACATCGCCAAGCTGTTCCAGTCC
GTGACCAGTGCCGAGTCCAAGGCCGATGAGCGCGGCTCGATCACGGTGGACGACCGGACCAACAACATCATTGCCTACCA
GACCCAGGAACGACTCGACGAGCTGCGTCGTATCGTTTCGCAACTGGACATCCCGGTACGCCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAGCTCGGGGTTCGCTGGGGCGGCTCTACGAACACCAGCGGTAACAGA
AAATGGACAACCTACGGGCTTGACAATAATGGCGACGAGGCCGGTAACACCAGCGGCAACCTGACCTCCAACGTGCCGTT
CGTGGACCTGGGCGCAGCCGGCGCCACCTCGGGTATCGGCCTTGGTTTCGTGACCAACAACACGTTGCTGGACCTTGAAC
TGAGTGCCATGGAGAAAACCGGCAACGGTGAAATCGTCTCCCAGCCCAAGGTGGTCACTTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGTACGGAGATCCCGTATCAGGAATCCAGCTCCAGCGGTGCGACCACCGTGAGCTTCAAGGAGGCTTCCCT
GTCGCTTGAAGTGACGCCGCAGATCACGCCGGACAATCGCATCATCATGGAAGTGAAGGTCACCAAGGACGAGCCGGACT
ACCTGAATGCGGTGCTGGGTGTGCCGCCGATCAAGAAGAACGAGGTCAACGCCAAAGTGCTGATTTCCGACGGCGAGACC
ATCGTCATCGGTGGTGTGTTCTCGAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCTGAGTCCAAATCCGAGCTGTTGGTATTTCTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterProScan.

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.333

100

0.753
  comQ Acinetobacter baylyi ADP1

41.917

99.715

0.418
  pilQ Acinetobacter baumannii D1279779

38.451

100

0.404
  pilQ Acinetobacter baumannii strain A118

38.451

100

0.404
  pilQ Legionella pneumophila strain Lp02

38.439

98.716

0.379
  pilQ Legionella pneumophila strain ERS1305867

38.15

98.716

0.377