Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   R6238_RS12410 Genome accession   NZ_CP138212
Coordinates   2935977..2937041 (-) Length   354 a.a.
NCBI ID   WP_003341394.1    Uniprot ID   A0A0P9GH14
Organism   Pseudomonas syringae pv. syringae strain pss-2019     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2930977..2942041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6238_RS12385 aroK 2931576..2932094 (-) 519 WP_003341387.1 shikimate kinase AroK -
  R6238_RS12390 pilQ 2932099..2934204 (-) 2106 WP_024959592.1 type IV pilus secretin PilQ Machinery gene
  R6238_RS12395 pilP 2934252..2934779 (-) 528 WP_003316653.1 type 4a pilus biogenesis lipoprotein PilP -
  R6238_RS12400 pilO 2934776..2935399 (-) 624 WP_003316652.1 type 4a pilus biogenesis protein PilO -
  R6238_RS12405 - 2935396..2935977 (-) 582 WP_024959591.1 PilN domain-containing protein -
  R6238_RS12410 pilM 2935977..2937041 (-) 1065 WP_003341394.1 pilus assembly protein PilM Machinery gene
  R6238_RS12415 - 2937251..2939692 (+) 2442 WP_373365899.1 penicillin-binding protein 1A -
  R6238_RS12420 - 2939922..2941190 (-) 1269 WP_003316648.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38134.68 Da        Isoelectric Point: 4.7090

>NTDB_id=901443 R6238_RS12410 WP_003341394.1 2935977..2937041(-) (pilM) [Pseudomonas syringae pv. syringae strain pss-2019]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLEPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=901443 R6238_RS12410 WP_003341394.1 2935977..2937041(-) (pilM) [Pseudomonas syringae pv. syringae strain pss-2019]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCATTGCCGGCCAACGCGGTCGTCGAAAAGAACA
TTGCCGAACTCGAAGGGGTCGGGCAGGCGTTGTCACGCGTGCTCGTCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTT
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGGCTTTCCGACGATGACATGGAAAACCAGCT
CAAGCTTGAAGCTGACCAGTACATCCCCTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTATTCGGTGC
GCAACCCCGAGCGCGTCGAGGTGTTACTGGCTGCCTGCCGCAAGGAAAACGTCGAAGTACGCGAGGCCGCGCTGGCACTG
GCCGGGCTGACCGCACGCGTCGTCGATGTCGAGGCCTACGCCCTTGAGCGTTCGTTCGGTTTGCTGGCGGCGCAACTGGG
CAATGGCCACGACGAGCTGACGGTGGCCGTGGTCGATATCGGCGCCACCATGACCACCCTGAGCGTGCTGCATCATGGCC
GGATCATCTATACCCGCGAGCAGCTGTTTGGTGGGCGCCAGTTGACTGACGAAATCCAGCGCCGCTACGGGCTCTCCATG
GAGGAAGCCGGGCTTGCCAAGAAGCAGGGCGGCTTGCCCGACGATTACGTCAGCGAGGTGCTCGAGCCCTTCAAGGAAGC
GCTGGTGCAGCAGGTTTCCCGATCCCTGCAGTTCTTCTTCGCGGCCGGTCAATACAACTCAGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTTGAGCATCTGATCCAGCGGCGCATCGGCACGCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCACTGAGCTCCAAGGTCAATGCCGGCGCCCTGGCCAGTGATGCGCCGGCGCTGATGATCGCGTGCGG
CCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.65

100

0.557
  comM Acinetobacter nosocomialis M2

55.65

100

0.557
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

46.761

100

0.469