Detailed information NTG0901118

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: SBK94_RS12725
• Genome accession: NZ_CP137889
• Coordinates: 2594352..2594966 (-)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain N23
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2589352..2599966

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SBK94_RS12695 (SBK94_12695)	rpsT	2589652..2589918 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
SBK94_RS12700 (SBK94_12700)	holA	2589935..2590976 (-)	1042	Protein_2462	DNA polymerase III subunit delta	-
SBK94_RS12705 (SBK94_12705)	-	2591016..2591170 (-)	155	Protein_2463	hypothetical protein	-
SBK94_RS12710 (SBK94_12710)	-	2591211..2591345 (+)	135	WP_003152870.1	YqzM family protein	-
SBK94_RS12715 (SBK94_12715)	comEC	2591364..2593715 (-)	2352	WP_041481887.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SBK94_RS12720 (SBK94_12720)	-	2593716..2594285 (-)	570	WP_003152868.1	ComE operon protein 2	-
SBK94_RS12725 (SBK94_12725)	comEA	2594352..2594966 (-)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
SBK94_RS12730 (SBK94_12730)	comER	2595025..2595846 (+)	822	WP_014305449.1	late competence protein ComER	-
SBK94_RS12735 (SBK94_12735)	-	2595915..2596652 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
SBK94_RS12740 (SBK94_12740)	rsfS	2596649..2597005 (-)	357	WP_014305451.1	ribosome silencing factor	-
SBK94_RS12745 (SBK94_12745)	yqeK	2597002..2597583 (-)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
SBK94_RS12750 (SBK94_12750)	-	2597573..2598142 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
SBK94_RS12755 (SBK94_12755)	yhbY	2598153..2598443 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
SBK94_RS12760 (SBK94_12760)	aroE	2598437..2599279 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=901118 SBK94_RS12725 WP_003152867.1 2594352..2594966(-) (comEA) [Bacillus velezensis strain N23]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=901118 SBK94_RS12725 WP_003152867.1 2594352..2594966(-) (comEA) [Bacillus velezensis strain N23]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363