Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   SBK94_RS12715 Genome accession   NZ_CP137889
Coordinates   2591364..2593715 (-) Length   783 a.a.
NCBI ID   WP_041481887.1    Uniprot ID   -
Organism   Bacillus velezensis strain N23     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2586364..2598715
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SBK94_RS12680 (SBK94_12680) - 2586727..2587065 (-) 339 WP_003152882.1 YqxA family protein -
  SBK94_RS12685 (SBK94_12685) spoIIP 2587082..2588275 (-) 1194 WP_014305446.1 stage II sporulation protein P -
  SBK94_RS12690 (SBK94_12690) gpr 2588343..2589449 (-) 1107 WP_003152878.1 GPR endopeptidase -
  SBK94_RS12695 (SBK94_12695) rpsT 2589652..2589918 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  SBK94_RS12700 (SBK94_12700) holA 2589935..2590976 (-) 1042 Protein_2462 DNA polymerase III subunit delta -
  SBK94_RS12705 (SBK94_12705) - 2591016..2591170 (-) 155 Protein_2463 hypothetical protein -
  SBK94_RS12710 (SBK94_12710) - 2591211..2591345 (+) 135 WP_003152870.1 YqzM family protein -
  SBK94_RS12715 (SBK94_12715) comEC 2591364..2593715 (-) 2352 WP_041481887.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  SBK94_RS12720 (SBK94_12720) - 2593716..2594285 (-) 570 WP_003152868.1 ComE operon protein 2 -
  SBK94_RS12725 (SBK94_12725) comEA 2594352..2594966 (-) 615 WP_003152867.1 helix-hairpin-helix domain-containing protein Machinery gene
  SBK94_RS12730 (SBK94_12730) comER 2595025..2595846 (+) 822 WP_014305449.1 late competence protein ComER -
  SBK94_RS12735 (SBK94_12735) - 2595915..2596652 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  SBK94_RS12740 (SBK94_12740) rsfS 2596649..2597005 (-) 357 WP_014305451.1 ribosome silencing factor -
  SBK94_RS12745 (SBK94_12745) yqeK 2597002..2597583 (-) 582 WP_014305452.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  SBK94_RS12750 (SBK94_12750) - 2597573..2598142 (-) 570 WP_014305453.1 nicotinate-nucleotide adenylyltransferase -
  SBK94_RS12755 (SBK94_12755) yhbY 2598153..2598443 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 783 a.a.        Molecular weight: 86621.64 Da        Isoelectric Point: 9.0078

>NTDB_id=901116 SBK94_RS12715 WP_041481887.1 2591364..2593715(-) (comEC) [Bacillus velezensis strain N23]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIMIKTKQPAPVVVCLVSFCVYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATIPGGFDYKEYLYSQQI
HWLFTVTSIQQCEKSKQPLFKLLSIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGMLLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YLLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLASFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFNMFDLVMKPVHDFITYAASVDLFTVIVLKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFIPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGKSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPKLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFEKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide


Download         Length: 2352 bp        

>NTDB_id=901116 SBK94_RS12715 WP_041481887.1 2591364..2593715(-) (comEC) [Bacillus velezensis strain N23]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATGATAAAAACAAAGCAGCCTGCTCCGGTTGTTGTCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGGTCTCTTGAGGAAAAGAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGATTCCCGGGGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTACCGTGACTTCTATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAGCATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAAGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGACTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGGATGTTGCTGCTGCT
GTTTTTGCCGGTCTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCTCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACCTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCAGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAAATTTCACTTGCCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTTTCAAGGCAGATGGGAGAATGTTTGTTTAATATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTGTGATTGTGTTAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTAGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCCGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCATTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCGCTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAGGATCACATCGGGGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAAGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACAGAAGTGCTGAAAACGTATCCGAAACTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCACGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTGAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

Metallo-beta-lactamase superfamily (Lactamase_B)

(508-719)

Competence protein (Competence)

(208-469)

Domain of unknown function (DUF4131) (DUF4131)

(25-171)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

56.347

98.595

0.556