Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   R5H22_RS20360 Genome accession   NZ_CP137764
Coordinates   4427935..4430034 (-) Length   699 a.a.
NCBI ID   WP_175555950.1    Uniprot ID   -
Organism   Azotobacter sp. NL3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4422935..4435034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5H22_RS20345 (R5H22_20320) - 4424596..4426249 (-) 1654 Protein_3990 AAA family ATPase -
  R5H22_RS20350 (R5H22_20325) aroB 4426257..4427360 (-) 1104 WP_012703005.1 3-dehydroquinate synthase -
  R5H22_RS20355 (R5H22_20330) aroK 4427412..4427930 (-) 519 WP_012703006.1 shikimate kinase AroK -
  R5H22_RS20360 (R5H22_20335) pilQ 4427935..4430034 (-) 2100 WP_175555950.1 type IV pilus secretin PilQ Machinery gene
  R5H22_RS20365 (R5H22_20340) - 4430088..4430612 (-) 525 WP_012703008.1 pilus assembly protein PilP -
  R5H22_RS20370 (R5H22_20345) pilO 4430609..4431229 (-) 621 WP_012703009.1 type 4a pilus biogenesis protein PilO -
  R5H22_RS20375 (R5H22_20350) - 4431226..4431885 (-) 660 WP_012703010.1 PilN domain-containing protein -
  R5H22_RS20380 (R5H22_20355) pilM 4431886..4432950 (-) 1065 WP_041807416.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75789.05 Da        Isoelectric Point: 4.7057

>NTDB_id=900611 R5H22_RS20360 WP_175555950.1 4427935..4430034(-) (pilQ) [Azotobacter sp. NL3]
MDSTLSRLGILVLTALLSPELMAADLESLDVAALPGDRVELKLSFDEAVPAPRGYTIDQPARIALDLPGVTNKLRTKYRE
LGVGNARSVTLVEASGRTRMIVNLAAMSPYSTRSEGHDLYVLIGESATVTRQSRSASLAVAQAKPYVTPGKAVRGIDFQR
GEQGEGNVIIDLSSPGMNPDLQEQGGKIRLDFPGTQLPESLRARLDVKDFATPVHFVSASSQAGKASILIEPAGMYDYLV
YQADKRLTVSVKAKPQDSGAGRDAGRFAYTGERLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPWDQA
LDLVLRTKGLDKRKIGNVLLVAPADEIAARERQELEAQQQIAELAPLRRELLQVNYAKATDIAKLFQSVTSSDEVEEQRG
SITVDERTNNIIAYQTQDRLDELRRIVAQLDVPVRQVMIEARIVEANVDYDKSLGVRWGGSFVNDQWSLYGKDGAQSVDD
GQVFLPGTGQVGNFTLEDGMAPVPFVDMGVANSTSGLGIGFISDHLILDLQLSAMEKTGNGEVISQPKVLTSDKETAKIL
TGEEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDQADFAKALNGVPPINKNEVNAKVLIGDGETIV
IGGVFSNIQAKSVDKVPFLGDLPFIGRLFRRDVVADSKSELLVFLTPRILNSQAIAVSR

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=900611 R5H22_RS20360 WP_175555950.1 4427935..4430034(-) (pilQ) [Azotobacter sp. NL3]
ATGGACAGCACCCTTTCGCGTCTCGGTATTTTGGTCCTGACCGCCCTGCTGTCGCCGGAATTGATGGCGGCCGATCTCGA
GTCCCTGGACGTGGCGGCCCTGCCTGGCGATCGGGTCGAACTCAAGCTGTCATTCGACGAGGCCGTGCCGGCGCCACGTG
GCTACACCATCGACCAGCCGGCCCGGATTGCCCTGGATCTTCCCGGCGTGACCAACAAGCTGAGGACCAAGTACCGCGAG
TTGGGCGTCGGCAACGCCCGCAGTGTCACCCTGGTCGAGGCGAGCGGGCGCACCCGGATGATCGTCAACCTGGCCGCGAT
GTCGCCCTACAGCACGCGGAGCGAGGGCCATGACCTTTATGTGCTGATCGGCGAGTCGGCCACCGTGACCCGGCAGTCCC
GCAGTGCCTCGCTCGCGGTCGCGCAGGCCAAGCCCTACGTAACGCCAGGCAAGGCGGTTCGGGGCATCGACTTCCAGCGT
GGCGAGCAGGGCGAAGGCAACGTCATCATCGACCTGTCGAGCCCGGGCATGAATCCCGACCTCCAGGAGCAGGGTGGCAA
GATCCGCCTGGACTTTCCGGGAACGCAGTTGCCGGAGTCCCTGCGGGCACGCCTGGACGTCAAGGATTTCGCCACCCCCG
TGCACTTCGTGAGTGCCTCGAGCCAGGCCGGCAAGGCCAGCATCCTCATCGAGCCGGCCGGCATGTATGACTATCTGGTC
TACCAGGCCGACAAGCGACTGACCGTCAGTGTCAAGGCGAAGCCCCAGGACAGCGGAGCCGGGCGTGATGCCGGTCGCTT
CGCGTATACCGGCGAGAGGCTGTCGCTGAATTTCCAGGATATCGATGTCCGTTCGGTCCTGCAGTTGATCGCCGATTTCA
CCGATCTCAACCTGGTGGCCAGCGACACGGTACAGGGCAATATCACCCTGCGCCTGCAGAATGTGCCCTGGGATCAGGCG
CTCGACCTGGTGCTCAGGACCAAGGGATTGGACAAGCGCAAGATCGGCAACGTGCTGTTGGTGGCGCCGGCCGACGAAAT
CGCGGCACGCGAGCGCCAAGAGCTGGAGGCACAGCAGCAGATCGCCGAACTGGCCCCCCTGCGTCGCGAATTGCTCCAGG
TGAACTACGCCAAGGCGACGGACATCGCCAAACTGTTCCAGTCGGTCACCAGCAGCGATGAGGTGGAAGAGCAGCGCGGC
TCCATCACCGTCGACGAGCGGACCAACAATATCATCGCCTACCAGACCCAGGACCGTCTGGACGAGCTGCGGCGGATCGT
CGCCCAACTGGACGTCCCGGTGCGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAACGTCGATTACGACAAGAGCC
TGGGGGTGCGCTGGGGCGGAAGCTTCGTCAACGACCAGTGGAGCCTGTACGGCAAGGATGGCGCGCAATCCGTCGATGAC
GGACAGGTATTCCTGCCGGGAACCGGCCAGGTCGGCAACTTCACCCTGGAAGATGGCATGGCTCCCGTTCCCTTCGTCGA
CATGGGGGTTGCGAACAGTACTTCGGGTCTCGGCATCGGCTTCATTTCCGATCACCTGATCCTCGACCTCCAACTGTCGG
CCATGGAAAAGACCGGCAATGGAGAAGTCATTTCCCAGCCCAAGGTTCTCACCTCGGACAAGGAGACGGCGAAGATCCTG
ACCGGCGAGGAGATTCCCTACCAGGAGGCCAGCTCCAGCGGCGCGACCAGCACTTCCTTCAAGGAGGCGGCTCTGTCGCT
GGAGGTGACGCCGCAAATCACTCCCGACAACCGCATCATCATGGAGGTGAAGGTGACCAAGGATCAGGCGGACTTCGCCA
AGGCCCTGAACGGTGTGCCGCCGATCAACAAGAACGAGGTCAACGCCAAGGTGCTGATCGGCGACGGCGAAACCATCGTG
ATCGGCGGAGTCTTCTCCAATATCCAGGCCAAGTCCGTGGACAAGGTGCCGTTCCTCGGCGACCTGCCGTTCATCGGGCG
CCTGTTCCGCCGCGACGTGGTCGCCGACAGCAAATCCGAGCTGCTTGTTTTCCTGACTCCGCGTATTCTTAACAGCCAGG
CTATCGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(534-690)

AMIN domain (AMIN)

(37-121)

Secretin and TonB N terminus short domain (STN)

(297-343)

Bacterial type II/III secretion system short domain (Secretin_N)

(371-436)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.549

100

0.741
  pilQ Acinetobacter baumannii strain A118

41.092

99.571

0.409
  pilQ Acinetobacter baumannii D1279779

41.092

99.571

0.409
  comQ Acinetobacter baylyi ADP1

40.773

100

0.408
  pilQ Legionella pneumophila strain ERS1305867

37.593

96.28

0.362
  pilQ Legionella pneumophila strain Lp02

37.444

96.28

0.361