Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   R5H22_RS20380 Genome accession   NZ_CP137764
Coordinates   4431886..4432950 (-) Length   354 a.a.
NCBI ID   WP_041807416.1    Uniprot ID   -
Organism   Azotobacter sp. NL3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4426886..4437950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5H22_RS20355 (R5H22_20330) aroK 4427412..4427930 (-) 519 WP_012703006.1 shikimate kinase AroK -
  R5H22_RS20360 (R5H22_20335) pilQ 4427935..4430034 (-) 2100 WP_175555950.1 type IV pilus secretin PilQ Machinery gene
  R5H22_RS20365 (R5H22_20340) - 4430088..4430612 (-) 525 WP_012703008.1 pilus assembly protein PilP -
  R5H22_RS20370 (R5H22_20345) pilO 4430609..4431229 (-) 621 WP_012703009.1 type 4a pilus biogenesis protein PilO -
  R5H22_RS20375 (R5H22_20350) - 4431226..4431885 (-) 660 WP_012703010.1 PilN domain-containing protein -
  R5H22_RS20380 (R5H22_20355) pilM 4431886..4432950 (-) 1065 WP_041807416.1 pilus assembly protein PilM Machinery gene
  R5H22_RS20385 (R5H22_20360) - 4433132..4435591 (+) 2460 WP_012703012.1 penicillin-binding protein 1A -
  R5H22_RS20390 (R5H22_20365) - 4435708..4437033 (+) 1326 WP_081434910.1 IS4-like element ISAzvi6 family transposase -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37587.48 Da        Isoelectric Point: 4.6047

>NTDB_id=900612 R5H22_RS20380 WP_041807416.1 4431886..4432950(-) (pilM) [Azotobacter sp. NL3]
MLRLFNKQAGSLLGIDIGSTMVKLLELSRSGGGYRVEAYAVEPLPANAVVEKNIVDIDGVGQALGKVLARIGTGARRAAV
AVAGSVVITKVMEMDAGLSEEEMETQLTLEAEQYIPYPLEEVAIDFEVLGPLSRSPGRVEVLLVACRRESVEAREAVLAL
AGLTAGVVDIEAYAMERVCGLLIDRLGGDPSELTVAVVDIGATMMTLSVLHDGRILYTREQSFGGKQLTDEIQRRYGLSV
AEAGLARQKGGLPADCDSVVLQPFKEAVVQQISRSLQFFFAAGRYDQVDCILLAGGTSSLPGLDQLVQQKIGTRTLVANP
FAGMTLSPKVNAAALASDAPAMMVACGLAMRRFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=900612 R5H22_RS20380 WP_041807416.1 4431886..4432950(-) (pilM) [Azotobacter sp. NL3]
GTGCTAAGGCTCTTCAACAAGCAGGCGGGTTCATTGCTGGGAATCGATATCGGTTCGACCATGGTCAAACTTCTCGAATT
GAGTCGTTCGGGGGGCGGCTATCGGGTCGAGGCCTATGCTGTCGAACCGCTGCCGGCCAATGCCGTCGTCGAGAAGAATA
TCGTCGATATCGATGGTGTCGGTCAGGCCTTGGGCAAGGTGCTGGCCAGGATCGGGACCGGAGCCCGGCGTGCCGCGGTG
GCCGTGGCCGGTTCCGTGGTGATCACCAAGGTCATGGAGATGGATGCCGGGCTTTCCGAAGAGGAAATGGAAACCCAGTT
GACGCTCGAGGCCGAACAATACATTCCCTACCCCCTCGAGGAGGTGGCGATCGATTTCGAGGTCCTGGGGCCGCTGTCGC
GCAGTCCGGGCCGGGTCGAAGTGCTGCTCGTCGCTTGCCGCAGGGAGAGCGTCGAGGCCCGCGAGGCGGTGCTGGCGCTG
GCAGGCCTGACAGCCGGGGTCGTCGATATCGAGGCCTATGCCATGGAGCGTGTCTGCGGGCTGCTGATCGATCGGTTGGG
GGGCGACCCGTCGGAGTTGACCGTGGCCGTCGTCGATATCGGCGCGACCATGATGACGCTCAGCGTGTTGCATGATGGAC
GCATCCTCTATACCCGCGAGCAATCGTTCGGCGGCAAGCAGCTCACCGACGAGATCCAGCGCCGTTACGGACTGTCCGTG
GCCGAAGCCGGCTTGGCCCGGCAAAAGGGTGGACTCCCGGCCGACTGCGACAGCGTGGTCCTGCAACCTTTCAAGGAGGC
CGTCGTACAGCAGATATCCCGCTCGCTGCAGTTCTTCTTCGCGGCGGGCCGGTACGACCAGGTCGACTGCATCCTGCTGG
CCGGCGGGACCTCCTCCCTGCCGGGCCTCGACCAACTGGTCCAGCAGAAGATCGGCACCAGGACCCTGGTGGCCAATCCG
TTCGCCGGGATGACGCTGAGCCCCAAGGTGAATGCCGCGGCCTTGGCCAGCGACGCTCCGGCCATGATGGTCGCCTGCGG
CCTGGCGATGAGGCGCTTCGATTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.966

100

0.523
  comM Acinetobacter nosocomialis M2

51.966

100

0.523
  comM Acinetobacter baylyi ADP1

50

100

0.5
  pilM Legionella pneumophila strain ERS1305867

42.938

100

0.429