Detailed information NTG0899408

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: R6U80_RS09615
• Genome accession: NZ_CP137623
• Coordinates: 1977373..1978020 (-)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis strain NCK401
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1972373..1983020

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R6U80_RS09590 (R6U80_09590)	atpF	1972442..1972948 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
R6U80_RS09595 (R6U80_09595)	atpB	1972963..1973676 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
R6U80_RS09600 (R6U80_09600)	-	1973721..1973936 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
R6U80_RS09605 (R6U80_09605)	-	1974121..1974897 (-)	777	WP_023188533.1	alpha/beta hydrolase family protein	-
R6U80_RS09610 (R6U80_09610)	comEC	1975182..1977392 (-)	2211	WP_313791935.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
R6U80_RS09615 (R6U80_09615)	comEA	1977373..1978020 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
R6U80_RS09620 (R6U80_09620)	-	1978080..1979441 (-)	1362	WP_313791934.1	ABC transporter permease	-
R6U80_RS09625 (R6U80_09625)	-	1979438..1980370 (-)	933	WP_313791933.1	ABC transporter ATP-binding protein	-
R6U80_RS09630 (R6U80_09630)	-	1980476..1980874 (-)	399	WP_023188528.1	hypothetical protein	-
R6U80_RS09635 (R6U80_09635)	-	1980978..1981541 (-)	564	WP_251900925.1	GNAT family N-acetyltransferase	-
R6U80_RS09640 (R6U80_09640)	-	1981714..1982892 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=899408 R6U80_RS09615 WP_010906131.1 1977373..1978020(-) (comEA) [Lactococcus lactis strain NCK401]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=899408 R6U80_RS09615 WP_010906131.1 1977373..1978020(-) (comEA) [Lactococcus lactis strain NCK401]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386