Detailed information NTG0898718

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R5022_RS08160
• Genome accession: NZ_CP137514
• Coordinates: 1756285..1757433 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain HPA0170
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1751285..1762433

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5022_RS08135 (R5022_08135)	-	1752436..1752732 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
R5022_RS08140 (R5022_08140)	-	1752729..1753322 (-)	594	WP_003084544.1	YggT family protein	-
R5022_RS08145 (R5022_08145)	proC	1753333..1754154 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
R5022_RS08150 (R5022_08150)	-	1754166..1754858 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
R5022_RS08155 (R5022_08155)	pilT	1755073..1756107 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
R5022_RS08160 (R5022_08160)	pilU	1756285..1757433 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
R5022_RS08165 (R5022_08165)	czcD	1757441..1758340 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
R5022_RS08170 (R5022_08170)	-	1758495..1758899 (+)	405	WP_003084560.1	NINE protein	-
R5022_RS08175 (R5022_08175)	-	1759142..1760515 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
R5022_RS08180 (R5022_08180)	-	1760512..1761696 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=898718 R5022_RS08160 WP_003110016.1 1756285..1757433(+) (pilU) [Pseudomonas aeruginosa strain HPA0170]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=898718 R5022_RS08160 WP_003110016.1 1756285..1757433(+) (pilU) [Pseudomonas aeruginosa strain HPA0170]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATAACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382