Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   R4713_RS00705 Genome accession   NZ_CP137103
Coordinates   133586..134236 (-) Length   216 a.a.
NCBI ID   WP_000387328.1    Uniprot ID   A0A4N9N7Z7
Organism   Streptococcus pneumoniae strain 21334     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 128586..139236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4713_RS00685 - 129191..130372 (-) 1182 WP_000655951.1 membrane protein -
  R4713_RS00690 - 130376..130963 (-) 588 WP_000939884.1 ATP-binding cassette domain-containing protein -
  R4713_RS00695 - 130996..131220 (-) 225 WP_000583432.1 hypothetical protein -
  R4713_RS00700 comEC/celB 131362..133602 (-) 2241 WP_000942392.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  R4713_RS00705 comEA/celA/cilE 133586..134236 (-) 651 WP_000387328.1 ComEA family DNA-binding protein Machinery gene
  R4713_RS00710 - 134304..134873 (-) 570 WP_000443775.1 GNAT family N-acetyltransferase -
  R4713_RS00715 ald 135050..136161 (+) 1112 Protein_140 alanine dehydrogenase -
  R4713_RS00720 - 136462..136788 (-) 327 Protein_141 TfoX/Sxy family protein -
  R4713_RS00725 - 136791..137291 (-) 501 WP_000566986.1 GNAT family N-acetyltransferase -
  R4713_RS00730 - 137484..138452 (-) 969 WP_000658198.1 PhoH family protein -
  R4713_RS00735 - 138538..138753 (-) 216 WP_001232081.1 YozE family protein -

Sequence

Protein


Download         Length: 216 a.a.        Molecular weight: 23230.59 Da        Isoelectric Point: 5.0667

>NTDB_id=895302 R4713_RS00705 WP_000387328.1 133586..134236(-) (comEA/celA/cilE) [Streptococcus pneumoniae strain 21334]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEEEVNKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD

Nucleotide


Download         Length: 651 bp        

>NTDB_id=895302 R4713_RS00705 WP_000387328.1 133586..134236(-) (comEA/celA/cilE) [Streptococcus pneumoniae strain 21334]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGAGGATT
TTTCCTGCTAAAACCAGCTCCACAAACACCTGTCAAAGAGACGAATTTGCAGGCTGAAGTCGCAGCTGTTTCCAAGGATT
TGGTATCCGAAGAGGAAGTGAACAAGGAAGAAAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGCCAACAGACTGGTTTGGGGACAGCTTCTTCAATAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATCGACCATCGTGAGGCAAATGG
CAAGTTCAAGTCAGTAGACGAGCTCAAGAAGGTCTCTGGCATTGGTGGCAAAACAATAGAAAAGCTTAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(76-126)

Helix-hairpin-helix motif (HHH_3)

(151-214)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4N9N7Z7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus pneumoniae Rx1

99.537

100

0.995
  comEA/celA/cilE Streptococcus pneumoniae D39

99.537

100

0.995
  comEA/celA/cilE Streptococcus pneumoniae R6

99.537

100

0.995
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

96.759

100

0.968
  comEA/celA/cilE Streptococcus mitis NCTC 12261

95.833

100

0.958
  comEA/celA/cilE Streptococcus mitis SK321

89.815

100

0.898
  comEA Lactococcus lactis subsp. cremoris KW2

43.172

100

0.454
  comEA Bacillus subtilis subsp. subtilis str. 168

41.579

87.963

0.366