NTDB

Overview

Name	comEA/celA/cilE	Type	Machinery gene
Locus tag	R4714_RS10000	Genome accession	NZ_CP137099
Coordinates	1905531..1906181 (+)	Length	216 a.a.
NCBI ID	WP_000387332.1	Uniprot ID	A0A6I3U9C7
Organism	Streptococcus pneumoniae strain 20824-4
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1900531..1911181

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R4714_RS09970	-	1901014..1901229 (+)	216	WP_001232082.1	YozE family protein	-
R4714_RS09975	-	1901315..1902283 (+)	969	WP_000658183.1	PhoH family protein	-
R4714_RS09980	-	1902476..1902976 (+)	501	WP_000566988.1	GNAT family N-acetyltransferase	-
R4714_RS09985	-	1902979..1903305 (+)	327	Protein_1922	TfoX/Sxy family protein	-
R4714_RS09990	ald	1903606..1904717 (-)	1112	Protein_1923	alanine dehydrogenase	-
R4714_RS09995	-	1904894..1905463 (+)	570	WP_000443775.1	GNAT family N-acetyltransferase	-
R4714_RS10000	comEA/celA/cilE	1905531..1906181 (+)	651	WP_000387332.1	helix-hairpin-helix domain-containing protein	Machinery gene
R4714_RS10005	comEC/celB	1906165..1908405 (+)	2241	WP_000942425.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
R4714_RS10010	-	1908584..1908772 (+)	189	WP_044812623.1	hypothetical protein	-
R4714_RS10015	-	1908805..1909392 (+)	588	WP_000939884.1	ATP-binding cassette domain-containing protein	-
R4714_RS10020	-	1909396..1910577 (+)	1182	WP_054395574.1	hypothetical protein	-

Sequence

Protein

Download Length: 216 a.a. Molecular weight: 23219.57 Da Isoelectric Point: 5.5171

                                >NTDB_id=895058 R4714_RS10000 WP_000387332.1 1905531..1906181(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain 20824-4]

MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKG

AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA

SLEELKQVKGLGGKRAQDIIDHREANGKFKSIDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide

Download Length: 651 bp

                                >NTDB_id=895058 R4714_RS10000 WP_000387332.1 1905531..1906181(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain 20824-4]

ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGCGGATT

TTTCCTGCTAAAGCCAGCTCCACAAACACCTGTAAAGGAAACGAATTTGCAGGCTGAAGTTGCAGCTGTTTCCAAGGATT

TGGTATCCGAAAAGGAAGTGAACAAGGAAGAGAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT

GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC

AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG

AAGAAGCAGTTAGTCAACAGACTGGTTCGGGGACAGCTTCTTCAACAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC

AGTCTGGAAGAGCTTAAACAGGTTAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATAGACCATCGTGAGGCAAATGG

GAAATTCAAGTCGATTGATGAACTCAAGAAGGTCTCTGGCATTGGTGCTAAGACCATAGAAAAGCTAAAAGACTATGTTA

CAGTGGATTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(151-214)

SLBB domain (SLBB)

(76-126)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A6I3U9C7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA/celA/cilE	Streptococcus pneumoniae Rx1	98.148	100	0.981
comEA/celA/cilE	Streptococcus pneumoniae D39	98.148	100	0.981
comEA/celA/cilE	Streptococcus pneumoniae R6	98.148	100	0.981
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	97.222	100	0.972
comEA/celA/cilE	Streptococcus mitis NCTC 12261	96.759	100	0.968
comEA/celA/cilE	Streptococcus mitis SK321	91.204	100	0.912
comEA	Lactococcus lactis subsp. cremoris KW2	44.053	100	0.463
comEA	Latilactobacillus sakei subsp. sakei 23K	34.335	100	0.37
comEA	Bacillus subtilis subsp. subtilis str. 168	42.105	87.963	0.37

Detailed information NTG0895058