Detailed information NTG0893540

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: RJD36_RS03070
• Genome accession: NZ_CP136943
• Coordinates: 563994..564659 (+)
• Length: 221 a.a.
• NCBI ID: WP_013851600.1
• Uniprot ID: F5X5G1
• Organism: Streptococcus pasteurianus strain WUSP082
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 558994..569659

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RJD36_RS03040 (RJD36_03040)	-	559070..560248 (+)	1179	WP_048791494.1	MFS transporter	-
RJD36_RS03045 (RJD36_03045)	-	560303..561163 (+)	861	WP_048791495.1	helix-turn-helix transcriptional regulator	-
RJD36_RS03050 (RJD36_03050)	-	561222..561796 (+)	575	Protein_532	ROK family protein	-
RJD36_RS03055 (RJD36_03055)	-	562028..562309 (-)	282	WP_003063819.1	GIY-YIG nuclease family protein	-
RJD36_RS03060 (RJD36_03060)	-	562284..563045 (-)	762	WP_003063822.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
RJD36_RS03065 (RJD36_03065)	-	563153..563899 (+)	747	WP_003063827.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
RJD36_RS03070 (RJD36_03070)	comEA	563994..564659 (+)	666	WP_013851600.1	helix-hairpin-helix domain-containing protein	Machinery gene
RJD36_RS03075 (RJD36_03075)	comEC/celB	564652..566883 (+)	2232	WP_117479310.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
RJD36_RS03080 (RJD36_03080)	holA	566957..567994 (+)	1038	WP_003063837.1	DNA polymerase III subunit delta	-
RJD36_RS03085 (RJD36_03085)	sodA	568083..568691 (+)	609	WP_013851603.1	superoxide dismutase SodA	-
RJD36_RS03090 (RJD36_03090)	-	568840..569565 (+)	726	WP_013851604.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Sequence

Protein

• Length: 221 a.a.
• Molecular weight: 23571.26 Da
• Isoelectric Point: 4.2822

>NTDB_id=893540 RJD36_RS03070 WP_013851600.1 563994..564659(+) (comEA) [Streptococcus pasteurianus strain WUSP082]
MIEEIKEKLLENKTLAAVLGIILVMIIGFFAWLNVTKTTADAQNDLPALSTTFSTSSSASDLKTERSSSQTETQKVFVDI
KGAVKNEGVYELSNGSRVTDVVKLAGGFTEDADKKSVNLAEKVTDESVIYVARVDENVAPATTNSQANGSAQQEESSDKI
NLNTATLAELQTISGIGAKRAQDIIDYRDANGGFSSVDDLANVSGIGEKTLEKLKSEVTVD

Nucleotide

• Length: 666 bp

>NTDB_id=893540 RJD36_RS03070 WP_013851600.1 563994..564659(+) (comEA) [Streptococcus pasteurianus strain WUSP082]
ATGATTGAAGAAATAAAAGAAAAACTTTTGGAGAACAAAACGTTGGCAGCGGTATTGGGGATAATACTTGTCATGATAAT
CGGATTTTTTGCTTGGTTAAATGTGACAAAAACAACAGCGGATGCGCAAAATGACCTACCTGCCCTAAGCACGACTTTTT
CGACAAGTAGTAGCGCAAGTGATTTAAAAACAGAACGTTCAAGTTCGCAAACTGAGACTCAAAAAGTGTTTGTAGATATC
AAAGGCGCTGTGAAAAATGAGGGGGTTTATGAGCTTTCAAACGGTAGCCGTGTGACAGATGTGGTCAAATTAGCAGGTGG
TTTTACAGAAGATGCTGATAAGAAGTCGGTGAATCTGGCTGAAAAAGTAACCGATGAATCGGTGATTTATGTGGCAAGAG
TTGATGAAAATGTTGCTCCTGCAACGACTAATTCACAAGCAAATGGCAGCGCTCAACAGGAAGAAAGTTCAGATAAAATC
AATCTTAATACCGCAACTTTGGCGGAACTCCAGACGATTTCTGGCATTGGCGCCAAACGTGCCCAAGATATTATTGATTA
CCGTGATGCTAATGGTGGTTTTTCATCAGTTGATGACTTGGCGAATGTGTCTGGTATCGGAGAAAAAACACTAGAAAAGC
TAAAATCTGAGGTGACCGTTGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (157-219)

• SLBB domain (SLBB) (78-131)

3D structure

• AlphaFold DB: F5X5G1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Streptococcus thermophilus LMD-9	47.699	100	0.516
comEA/celA/cilE	Streptococcus mitis SK321	46.154	100	0.462
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	44.545	99.548	0.443
comEA/celA/cilE	Streptococcus mitis NCTC 12261	43.891	100	0.439
comEA/celA/cilE	Streptococcus pneumoniae Rx1	43.636	99.548	0.434
comEA/celA/cilE	Streptococcus pneumoniae D39	43.636	99.548	0.434
comEA/celA/cilE	Streptococcus pneumoniae R6	43.636	99.548	0.434
comEA	Latilactobacillus sakei subsp. sakei 23K	37.946	100	0.385