Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   R1T29_RS03150 Genome accession   NZ_CP136903
Coordinates   640900..641313 (-) Length   137 a.a.
NCBI ID   WP_029831396.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain X1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 635900..646313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1T29_RS03130 (R1T29_03130) coaE 636418..637032 (-) 615 WP_203503821.1 dephospho-CoA kinase -
  R1T29_RS03135 (R1T29_03135) pilD 637033..637902 (-) 870 WP_025552578.1 A24 family peptidase Machinery gene
  R1T29_RS03140 (R1T29_03140) pilC 637967..639190 (-) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  R1T29_RS03145 (R1T29_03145) pilB 639214..640899 (-) 1686 WP_203503823.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R1T29_RS03150 (R1T29_03150) pilA 640900..641313 (-) 414 WP_029831396.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  R1T29_RS03155 (R1T29_03155) nadC 641576..642463 (-) 888 WP_029798874.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R1T29_RS03160 (R1T29_03160) ampD 642556..643107 (+) 552 WP_203503996.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  R1T29_RS03165 (R1T29_03165) pdhR 643513..644280 (+) 768 WP_029823257.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 13859.75 Da        Isoelectric Point: 8.4778

>NTDB_id=893293 R1T29_RS03150 WP_029831396.1 640900..641313(-) (pilA) [Vibrio parahaemolyticus strain X1]
MKQSKQKKQQGFTLIELMIVVAIIGVLSAIAVPAYKDYVSKSELASGFATIKSVITPAELYIQENGAISGASPSDLGVSA
GANSLGTLSISGNAVVFTHNNGAVSGAAFTYSRGTSSGWTCALTGQPTGVDAPKGCS

Nucleotide


Download         Length: 414 bp        

>NTDB_id=893293 R1T29_RS03150 WP_029831396.1 640900..641313(-) (pilA) [Vibrio parahaemolyticus strain X1]
ATGAAACAGAGTAAACAGAAAAAACAACAAGGTTTTACCTTGATTGAATTGATGATTGTAGTGGCAATTATTGGTGTACT
GTCCGCAATTGCAGTCCCAGCATATAAAGATTATGTATCAAAGAGTGAGTTAGCATCTGGTTTCGCTACCATAAAATCAG
TAATCACTCCTGCCGAATTATATATACAAGAAAATGGCGCTATATCTGGAGCAAGCCCTAGTGACCTAGGAGTATCTGCT
GGTGCCAACAGCCTAGGAACACTATCTATCAGCGGTAATGCCGTTGTATTTACTCACAACAATGGAGCTGTATCTGGTGC
GGCTTTCACATATAGCAGAGGCACTAGTTCCGGTTGGACATGTGCTTTGACAGGACAACCAACTGGGGTTGATGCTCCAA
AAGGTTGCTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

51.408

100

0.533
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.474

100

0.438
  pilA Vibrio cholerae strain A1552

39.474

100

0.438
  pilA Vibrio cholerae C6706

39.474

100

0.438
  pilA Acinetobacter baumannii strain A118

40.69

100

0.431
  pilA Pseudomonas aeruginosa PAK

38

100

0.416