Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R0137_RS07435 Genome accession   NZ_CP136865
Coordinates   1690458..1692179 (-) Length   573 a.a.
NCBI ID   WP_407329749.1    Uniprot ID   -
Organism   Congregibacter brevis strain IMCC45268     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1685458..1697179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R0137_RS07410 (R0137_07375) argJ 1685959..1687197 (+) 1239 WP_407329741.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  R0137_RS07415 (R0137_07380) mutT 1687204..1687635 (+) 432 WP_407329742.1 8-oxo-dGTP diphosphatase MutT -
  R0137_RS07420 (R0137_07385) coaE 1687632..1688252 (-) 621 WP_407329744.1 dephospho-CoA kinase -
  R0137_RS07425 (R0137_07390) pilD 1688249..1689130 (-) 882 WP_407329746.1 prepilin peptidase Machinery gene
  R0137_RS07430 (R0137_07395) pilC 1689184..1690401 (-) 1218 WP_407329748.1 type II secretion system F family protein Machinery gene
  R0137_RS07435 (R0137_07400) pilB 1690458..1692179 (-) 1722 WP_407329749.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R0137_RS07440 (R0137_07405) - 1692552..1693019 (+) 468 WP_407329751.1 pilin -
  R0137_RS07445 (R0137_07410) - 1693147..1695066 (+) 1920 WP_407329752.1 hypothetical protein -
  R0137_RS07450 (R0137_07415) - 1695063..1696817 (+) 1755 WP_407329754.1 hypothetical protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62815.40 Da        Isoelectric Point: 4.6967

>NTDB_id=893182 R0137_RS07435 WP_407329749.1 1690458..1692179(-) (pilB) [Congregibacter brevis strain IMCC45268]
MNERVSGKPSGLAGRLVIEGLLDADQASKAQAAAANERIPFVRYLVDEVKIDSHKLAELASQEFGAARFDISAFDSQFLP
QGVVEPELVTKHHALPLYSRGKRLFIAVSDPTNLAALDEIKFHTGLNTEAILVDEKSLQKAIAEWSEAQDDIGGGLDDLE
SDEFDEIDIGAVDDPGAEEGDDSSNVDETPIVRFVNKVLVDAIKQGASDIHFEPYEKDYRVRFRTDGVLREVVRPPRNLA
PRLSARLKVMSQMDISERRMPQDGRIQMKLSRNRSIDFRVNTLPTLFGEKIVLRILDPTSAQMGIDALGYDPDQKEMFMK
ALKQPQGMILVTGPTGSGKTVSLYTGLNILNEPERNISTAEDPVEINLSGINQVHVNAKVGLNFAEALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHLVLSTLHTNSAAETISRLLNMGVPAFNVATSVSLIIAQRLGRRLCKECAAPADIPHDTL
MDEGFTDEMLATATIMKAVGCDACKDGYKGRVGIYEVVRITPEIANTIMEDGNSLEISRQARAHGFADLRTSALRKCAEG
LISLEEVNRVTID

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=893182 R0137_RS07435 WP_407329749.1 1690458..1692179(-) (pilB) [Congregibacter brevis strain IMCC45268]
ATGAACGAACGAGTCAGCGGTAAACCCTCGGGCCTGGCAGGTCGACTAGTAATCGAGGGGCTTCTGGATGCCGACCAGGC
ATCAAAGGCGCAGGCCGCCGCAGCCAACGAGCGAATACCCTTCGTTCGCTACCTAGTTGATGAAGTTAAAATTGATAGCC
ACAAGCTCGCCGAACTGGCATCGCAAGAGTTCGGTGCCGCCCGCTTTGATATCAGTGCTTTTGATTCTCAATTTCTCCCT
CAAGGCGTCGTAGAGCCAGAGCTCGTCACCAAGCATCATGCTCTTCCTCTTTACAGCCGTGGCAAGCGTCTCTTCATTGC
GGTTTCTGACCCCACAAACCTTGCGGCCCTGGACGAAATCAAGTTCCACACCGGTCTCAATACCGAAGCTATTCTCGTTG
ACGAGAAGAGCCTACAAAAAGCGATTGCGGAATGGAGCGAGGCTCAGGACGACATCGGCGGCGGGCTCGACGATCTCGAG
TCCGATGAGTTCGACGAGATCGATATCGGGGCGGTTGACGACCCGGGCGCTGAGGAGGGAGACGATAGCAGTAACGTTGA
CGAGACCCCCATCGTTCGTTTCGTAAACAAGGTTCTTGTAGACGCTATTAAACAGGGTGCGTCGGATATTCACTTTGAGC
CCTATGAAAAAGATTATCGAGTCCGCTTTCGTACAGACGGCGTTCTGCGCGAAGTGGTTCGCCCGCCACGTAACCTGGCA
CCTCGCCTCTCCGCACGTTTGAAGGTGATGTCTCAGATGGATATCTCCGAGCGTCGCATGCCGCAGGACGGCCGTATCCA
GATGAAGCTTTCGCGAAACCGATCAATCGATTTTCGCGTCAACACCCTTCCAACACTTTTTGGCGAAAAAATCGTGCTGC
GGATCTTGGATCCTACAAGTGCACAAATGGGTATCGACGCCTTGGGCTACGATCCAGACCAAAAAGAAATGTTTATGAAG
GCGCTGAAACAGCCTCAGGGCATGATTCTTGTGACGGGTCCTACTGGCTCGGGTAAGACCGTCTCACTTTACACCGGTCT
CAACATCCTCAACGAGCCCGAGCGCAACATTTCGACGGCAGAGGACCCTGTAGAAATAAACCTCTCCGGCATCAATCAGG
TGCATGTAAACGCCAAGGTCGGGCTGAACTTCGCTGAAGCGCTCCGTTCATTCCTACGTCAAGATCCCGACATCATCATG
GTGGGAGAGATTCGCGACCTTGAAACTGCAGAAATCGCTATTAAGGCGGCGCAGACGGGTCACCTCGTACTTTCCACACT
CCACACCAATAGCGCAGCAGAAACCATAAGCCGCCTTCTCAACATGGGAGTCCCCGCTTTCAACGTCGCGACCTCAGTAA
GCTTGATCATCGCTCAGCGACTGGGACGACGTCTTTGCAAGGAGTGCGCAGCACCCGCCGATATACCTCATGACACGCTG
ATGGACGAGGGATTCACCGACGAGATGTTGGCGACAGCGACGATTATGAAAGCGGTTGGTTGCGACGCCTGTAAAGATGG
TTACAAAGGTCGCGTGGGCATTTACGAAGTCGTGCGAATCACGCCCGAAATCGCCAACACCATCATGGAAGACGGGAACT
CCTTGGAGATCTCACGACAAGCCCGCGCCCATGGGTTCGCGGATCTTCGAACTTCTGCACTGCGGAAATGTGCCGAAGGA
CTCATTAGTCTTGAGGAAGTAAACCGCGTTACGATTGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.996

99.302

0.576
  pilB Acinetobacter baylyi ADP1

56.89

98.778

0.562
  pilB Legionella pneumophila strain ERS1305867

53.723

98.429

0.529
  pilB Vibrio cholerae strain A1552

52.727

95.986

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

50.97

98.953

0.504
  pilB Vibrio campbellii strain DS40M4

51.619

91.623

0.473
  pilF Neisseria gonorrhoeae MS11

47.452

99.302

0.471
  pilB/pilB1 Synechocystis sp. PCC 6803

41.717

87.435

0.365